Sonic Hedgehog Signaling Mediates Resveratrol to Increase Proliferation of Neural Stem Cells After Oxygen-Glucose Deprivation/Reoxygenation Injury in Vitro

Cheng, Wei; Yu, Pingping; Wang, Li; Shen, Changbo; Song, Xiaosong; Chen, Jixiang; Tang, Fanren; Yang, Qin

doi:10.1159/000374009

Cited by 47 publications

(36 citation statements)

References 42 publications

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“…In the past, we also performed several studies [11,40,49,50] to demonstrate that resveratrol at 10 ~ 20 mg/kg can suppress severe acute pancreatitis and reduce inflammatory injury in rats. In our current report, we chose three concentration gradients (10,20, and 40 mg/kg) of resveratrol for animal administration. Our results show that the effect of 10 mg/kg resveratrol for adhesion prevention is better than that of the sodium hyaluronate gel (a commonly used reagent for adhesion prevention).…”

Section: Discussionmentioning

confidence: 99%

“…Three doses (10,20, or 40 mg/kg) of resveratrol were administered via the penile dorsal vein on the day of surgery for 1 week following surgery.…”

Section: Animal and Establishment Of Intra-abdominal Adhesion Modelmentioning

confidence: 99%

“…Since then, this natural compound has been identified in grapes, red wine, peanuts, mulberry, pine, berries, and other plants and has gradually attracted researchers' attention. Many studies have demonstrated [7][8][9][10] that resveratrol and its analogs or derivatives exhibit good anti-inflammatory activity, antioxidant activity, and protection from heart diseases, neurodegenerative diseases, and metabolic disorders. We conducted a series of experiments [11,12] to confirm that resveratrol can effectively suppress the body's response to inflammation and reduce inflammatory injury.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

Wei

Chen

Wang

et al. 2016

Cell Physiol Biochem

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Background: Intra-abdominal adhesions are a very common complication following abdominal surgery. Our previous studies have demonstrated that the inhibition of inflammation at the sites of peritoneal injury can prevent the formation of intra-abdominal adhesions. Resveratrol is a natural extract with a broad range of anti-inflammatory effects. Therefore, we propose that resveratrol can reduce the formation of intra-abdominal adhesions after surgery. The aim of this study was to investigate the effect of resveratrol on intra-abdominal adhesion prevention in a rat model with surgery-induced peritoneal adhesions. Materials and Methods: The cecum wall and its opposite parietal peritoneum were abraded following laparotomy to induce intra-abdominal adhesion formation. Varying doses of resveratrol were administered to the animals. On the eighth day after surgery, the adhesion score was assessed using a visual scoring system. Picrosirius red staining and a hydroxyproline assay were used to assess the amount of collagen deposition in the adhesion tissues. The levels of serum interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor beta-1 (TGF-β1) were determined by an enzyme-linked immunosorbent assay (ELISA). Western blotting was performed to determine the protein expression of TGF-β1, fibrinogen, and α-smooth muscle actin (α-SMA) in rat peritoneal adhesion tissue. Real-time RT-PCR was performed to quantify the mRNA expression of TGF-β1, fibrinogen, and α-SMA. Results: Resveratrol significantly reduced intra-abdominal adhesion formation and fibrin deposition in the rat model. Furthermore, resveratrol significantly reduced the serum levels of IL-6, TNF-α, and TGF-β1. The protein and mRNA expression of TGF-β1, fibrinogen, and α-SMA in the rat peritoneum and adhesion tissues were also down-regulated due to resveratrol intervention. Conclusion: Resveratrol can effectively prevent the formation of postoperative intra-abdominal adhesions in a rat model. This effect may be related to the suppression of inflammatory cytokine expression in the injured peritoneum by resveratrol. This study suggests that resveratrol may be a new and effective anti-adhesive agent that is worthy of further study and has potential application value.

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Section: Discussionmentioning

confidence: 99%

“…Three doses (10,20, or 40 mg/kg) of resveratrol were administered via the penile dorsal vein on the day of surgery for 1 week following surgery.…”

Section: Animal and Establishment Of Intra-abdominal Adhesion Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

Wei

Chen

Wang

et al. 2016

Cell Physiol Biochem

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“…The cell viability was performed by Cell Counting Kit-8 (CCK-8) kit (Dojindo, Kumamoto, Japan) according to the manufacturer's protocol [19]. HCAECs (5 × 10 4 /well) were incubated in 96-well plates with QL (0-1 µg/ml) and ox-LDL (150 μg/ml) for 24 h, then CCK-8(10 μL) reagent was added for 2 h at 37°C.…”

Section: Cell Viability Assaymentioning

confidence: 99%

“…The tube formation was assessed by precoated Matrigel 24-well culture plates, as previously described [19]. Matrigel (BD Bioscience, San Jose, CA, USA) was added to each well of the 96-well culture plates at 37°C for 1 h for polymerization.…”

Section: Tube Forming Assaymentioning

confidence: 99%

Qiliqiangxin improves oxidized low-density lipoprotein-induced human coronary artery endothelial cells injury

Du¹,

Lv²,

Meng³

et al. 2018

biomedicalresearch

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Qiliqiangxin (QL) has protective effect for the cardiovascular diseases treatment. This study aim to evaluate the effect of QL on the cell viability, cell apoptosis and caspase-3 activity in oxidized LDL (oxLDL)-induced Human Coronary Artery Endothelial Cells (HCAECs). HCAECs were treated with QL (0-1µg/ml) and oxLDL (150 μg/ml). The HCAECs viability was detected by cell proliferation assay and Lactate Dehydrogenase (LDH) release assay. The HCAECs apoptosis was evaluated by the annexin V-FITC assay. The activity of caspase-3 of HCAECs was measured by colorimetric caspase-3 assay. Cell migration assay and capillary-like tube formation assay on Matrigel were performed. Treatment of HCAECs to ox-LDL (150 μg/ml) decreased cell viability, stimulated apoptosis and enhanced caspase-3 activity. In addition, treatment with QL (0.5 µg/ml) alone did not affect cell viability and LDH release. Furthermore, the treatment with QL (0.5 µg/ml) significantly increased ox-LDL (150 μg/ml) decreased cell viability. Treatment with QL (0.5 µg/ml) reduced ox-LDL-stimulated apoptosis and caspase-3 activity in HCAECs in a dose-dependent manner. QL (0.5 µg/ml) attenuated ox-LDL (150 μg/ml) abolished cell migration and tube formation. Our study demonstrated that QL prevents ox-LDLinduced HCAECs injury by decreasing the apoptosis via caspase-3 activity.

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Generation of reactive astrocytes from NG2 cells is regulated by sonic hedgehog

Honsa

Valný

Kriška

et al. 2016

Glia

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NG2 cells, a fourth glial cell type in the adult mammalian central nervous system, produce oligodendrocytes in the healthy nervous tissue, and display wide differentiation potential under pathological conditions, where they could give rise to reactive astrocytes. The factors that control the differentiation of NG2 cells after focal cerebral ischemia (FCI) are largely unknown. Here, we used transgenic Cspg4-cre/Esr1/ROSA26Sortm14(CAG-tdTomato) mice, in which tamoxifen administration triggers the expression of red fluorescent protein (tomato) specifically in NG2 cells and cells derived therefrom. Differentiation potential (in vitro and in vivo) of tomato-positive NG2 cells from control or postischemic brains was determined using the immunohistochemistry, single cell RT-qPCR and patch-clamp method. The ischemic injury was induced by middle cerebral artery occlusion, a model of FCI. Using genetic fate-mapping method, we identified sonic hedgehog (Shh) as an important factor that influences differentiation of NG2 cells into astrocytes in vitro. We also manipulated Shh signaling in the adult mouse brain after FCI. Shh signaling activation significantly increased the number of astrocytes derived from NG2 cells in the glial scar around the ischemic lesion, while Shh signaling inhibition caused the opposite effect. Since Shh signaling modifications did not change the proliferation rate of NG2 cells, we can conclude that Shh has a direct influence on the differentiation of NG2 cells and therefore, on the formation and composition of a glial scar, which consequently affects the degree of the brain damage. GLIA 2016;64:1518-1531.

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Sonic Hedgehog Signaling Mediates Resveratrol to Increase Proliferation of Neural Stem Cells After Oxygen-Glucose Deprivation/Reoxygenation Injury in Vitro

Cited by 47 publications

References 42 publications

Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

Qiliqiangxin improves oxidized low-density lipoprotein-induced human coronary artery endothelial cells injury

Generation of reactive astrocytes from NG2 cells is regulated by sonic hedgehog

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