Sonic Spray Ionization Method for Atmospheric Pressure Ionization Mass Spectrometry

Hirabayashi, Atsumu; Sakairi, Masatoshi; Koizumi, Hideaki

doi:10.1021/ac00096a025

Cited by 274 publications

(248 citation statements)

References 8 publications

Supporting

Mentioning

237

Contrasting

Unclassified

Order By: Relevance

“…Following improvements in sensitivity or resolution, the method utilized here is applicable to other disease or oligosaccharide alterations. SSI-IT/MS can also be used to analyze sialyl oligosaccharides and distinguish isomers using MS n [12,13]; thus a combination of SSI-IT/MS and RP-HPLC should be widely applicable to N-glycan analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Sonic spray ionization ion trap mass spectrometry (SSI-IT/MS) ionizes mildly with nitrogen 4 gas flow [12] and is applicable to quantitative analysis of neutral and sialic oligosaccharides in the negative ion mode [13,14]. Thus we combined SSI-IT and MS in order to compare N-glycan profiles from whole sera of healthy women and female RA patients.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients

Nakagawa

Hato

Takegawa

et al. 2007

Journal of Chromatography B

View full text Add to dashboard Cite

Altered N-glycosylation occurs in many diseases. In rheumatoid arthritis (RA), for example, reduction in galactose residues in IgG and an increase in fucose residues in α1-acid glycoprotein have been observed. To further analysis of N-glycans in disease, we show N-glycan profiling from whole serum employing reversed phase high performance liquid chromatography/negative-ion mode by sonic spray ionization ion trap mass spectrometry with pyridylamination. Profiles from female 15 RA patients and 18 aged-matched healthy women were compared. The most significant change seen in RA was decreased levels of mono-galactosyl bi-antennary N-glycans, in agreement with previous reports regarding IgG.We also show previously unreported differences between isomers and increased tri-antennary oligosaccharides. These results indicate that LC-MS analysis of whole serum N-glycans can identify N-glycan alterations in RA and that this is a promising method both for studies of RA mechanisms and diagnosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients

Nakagawa

Hato

Takegawa

et al. 2007

Journal of Chromatography B

View full text Add to dashboard Cite

show abstract

“…Easy ambient sonic spray ionization (EASI) is based on sonic spray ionization whereby analytes in solution are sprayed from a capillary under the influence of a highpressure (~30 bar) nebulizing gas without the application of a high voltage [216]. These conditions generate micro-droplets containing an unbalanced charge distribution, that upon solvent vaporization, produce analyte ions identical to those observed with electrospray approaches.…”

Section: Easy Ambient Sonic Spray Ionization (Easi)mentioning

confidence: 99%

Surface analysis of lipids by mass spectrometry: More than just imaging

Ellis

Brown

Panhuis

et al. 2013

Progress in Lipid Research

View full text Add to dashboard Cite

Mass spectrometry is now an indispensable tool for lipid analysis and is arguably the driving force in the renaissance of lipid research. In its various forms, mass spectrometry is uniquely capable of resolving the extensive compositional and structural diversity of lipids in biological systems. Furthermore, it provides the ability to accurately quantify molecular-level changes in lipid populations associated with changes in metabolism and environment; bringing lipid science to the "omics" age. The recent explosion of mass spectrometry-based surface analysis techniques is fuelling further expansion of the lipidomics field. This is evidenced by the numerous papers published on the subject of mass spectrometric imaging of lipids in recent years. While imaging mass spectrometry provides new and exciting possibilities, it is but one of the many opportunities direct surface analysis offers the lipid researcher. In this review we describe the current state-ofthe-art in the direct surface analysis of lipids with a focus on tissue sections, intact cells and thin-layer chromatography substrates. The suitability of these different approaches towards analysis of the major lipid classes along with their current and potential applications in the field of lipid analysis are evaluated. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 AbstractMass spectrometry is now an indispensable tool for lipid analysis and is arguably the driving force in the renaissance of lipid research. In its various forms, mass spectrometry is uniquely capable of resolving the extensive compositional and structural diversity of lipids in biological systems. Furthermore, it provides the ability to accurately quantify molecular-level changes in lipid populations associated with changes in metabolism and environment;bringing lipid science to the "omics" age. The recent explosion of mass spectrometry-based surface analysis techniques is fuelling further expansion of the lipidomics field. This is evidenced by the numerous papers published on the subject of mass spectrometric imaging of lipids in recent years. While imaging mass spectrometry provides new and exciting possibilities, it is but one of the many opportunities direct surface analysis offers the lipid researcher. In this review we describe the current state-of-the-art in the direct surface analysis of lipids with a focus on tissue sections, intact cells and thin-layer chromatography substrates.The suitability of these different approaches towards analysis of the major lipid classes along with their current and potential applications in the field of lip...

show abstract

“…Sonic spray ionization (SSI) was developed in 1994 by Hirabayashi (Hirabayashi, Sakairi, & Koizumi, 1994, (2002) with permission from Internet Journal of Chemistry, copyright 2002. ) 1995) as a technique to generate molecular ions under a very wide range of solvent systems and flow rates.…”

Section: Sonic Spray Ionizationmentioning

confidence: 99%

Development of new methodologies for the mass spectrometry study of bioorganic macromolecules

Cristoni

Bernardi

2003

Mass Spectrometry Reviews

View full text Add to dashboard Cite

I. Introduction 370 II. Techniques That Are Usually Employed in the Study of Bioorganic Macromolecules 371 A. Proteins and Peptides 371 B. Oligonucleotides 374 C. Oligosaccharides and Glycoconjugates 375 D. Various Complexes (DNA–Protein, Antigen–Antibody, Macromolecules–Metals) 377 III. Common Problems and Developments in the Analysis of Bioorganic Macromolecules by Mass Spectrometry 377 A. Ionization Source Problems and Developments 377 1. Sensitivity 377 2. Problems with Buffers 379 3. Multicharge Effect 381 B. Mass Analyzer Problems and Developments 381 1. Linear Dynamic Range 381 2. Tandem Mass Spectrometry 382 3. Sensitivity 382 4. Resolution 383 5. Mass Accuracy 383 IV. New Promising Technologies 384 A. Interfaces and Ionization Sources 384 1. Improvements in the Coupling of Liquid‐Phase Separation Systems to Mass Spectrometry 384 2. AP‐MALDI 384 3. Deprotonant Agents 385 4. Sonic Spray Ionization 388 5. APCI without Corona Discharge 391 B. Mass Analyzers 393 1. Linear Quadrupole Ion Trap 394 2. TOF Analyzers with New Detectors 395 3. Multiple Mass Analyzers 396 V. Software for Data Treatment 397 VI. Conclusions and Future Developments 399 Acknowledgments 400 References 400 In recent years, mass spectrometry has been increasingly used for the analysis of various macromolecules of biological, biomedical, and biochemical interest. This increase has been made possible by two key developments: the advent of electrospray ionization (ESI) and matrix‐assisted laser desorption ionization (MALDI) sources. The two new techniques produce a significant increase in mass range and in sensitivity that led to the development of new applications and of new analyzer designs, software, and robotics. This review, apart from the description of the status of mass spectrometry in the analysis of bioorganic macromolecules, is mainly devoted to the illustration of the more recent promising techniques and on their possible future evolution. © 2003 Wiley Periodicals, Inc., Mass Spec Rev 22:369–406, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mas.10062

show abstract

Sonic Spray Ionization Method for Atmospheric Pressure Ionization Mass Spectrometry

Cited by 274 publications

References 8 publications

Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients

Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients

Surface analysis of lipids by mass spectrometry: More than just imaging

Development of new methodologies for the mass spectrometry study of bioorganic macromolecules

Contact Info

Product

Resources

About