Sonication: A new method for gene transfer to plants

Abstract

Joersbo, M, and Brunstedt, J, 1992, Sonication: A new method for gene transfer to plants. -Physiol. Plant. 85: 230-234.Sonieation is a novel method for gene transfer into plant protoplasts and intact plant cells. The mode of action of ultrasound and its chemical, biochemical and physiological effects, are reviewed. The state of the art of acotistic transformation is presented and possible mechanisms are discussed.

Agrobacterium-mediated in planta genetic transformation of sugarcane setts

“…Nearly 57.4 % of the BASTA-resistant plants expressed the gus A gene with a transformation efficiency of 21.6 % ( Table 3). Longer duration of sonication has an inhibitory effect on cells, such as cell lysis and suppression of RNA and protein synthesis (Joersbo and Brunstedt 1992). Chen et al (2010) reported 31.3 % transformation efficiency in Panicum virgatum by sonicating 3-day-old seedlings for 1 min.…”

Androgenesis-inducing stress treatments change phytohormone levels in anthers of three legume species (Fabaceae)

“…Electroporated microspores develop embryos faster for Gingko biloba L. (Laurain et al 1993), canola (Jardinaud et al 1993), pea ), and chickpea ). Similar to electroporation, sonication causes microcavity formation and collapse resulting in reversible permeability changes of cell membranes that facilitate the uptake of medium components (Fizzell 1988;Joersbo and Brunstedt 1992). Sonication at 38 kHz for 30 s enhances androgenetic callusing in pea but only if combined with other stresses (Ribalta et al 2012).…”

Ultrasound-mediated stable transformation of potato tuber discs

“…genes into plant cells protoplasts (.$sbo & Brunstedt, 1990 a,b,c;1992) and intact pieces of plant tissue (Zhang 1991). Since tissue culture derived from potato tuber discs can be regenerated to yield mature plants (Sheerman & Bevan, 1988), ultrasonication technology may have promise for the production of transgenic potato.…”