Sonication-Based Basic Protocol for Liposome Synthesis

Méndez, Roberto

doi:10.1007/978-1-0716-2966-6_31

Cited by 6 publications

(2 citation statements)

References 24 publications

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“…The resulting liposome suspension was then sonicated for 15 min at 65 °C to obtain small unilamellar vesicles (SUVs) . While liposomes can be susceptible to higher temperatures, the sonication parameters used in our investigation were chosen based on previous research and methodologies. − Visual inspection of the prepared liposomes incorporated gamma-oryzanol for changes to their physical characteristics, including color, transparency, and the presence of any visible particles or aggregates. Alteration in these visual attributes could indicate changes in the physical state of the liposomes, such as aggregation or degradation.…”

Section: Methodsmentioning

confidence: 99%

Liposome Nanocarriers Based on γ Oryzanol: Preparation, Characterization, and In Vivo Assessment of Toxicity and Antioxidant Activity

Jasim,

Albukhaty,

Sulaiman

et al. 2024

ACS Omega

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The present study aimed to develop and characterize liposome nanocarriers based on γ oryzanol and evaluate their potential in vitro and in vivo toxicity and antioxidant effects. The liposomes were physicochemically characterized using various techniques, including dynamic light scattering (DLS) for size and polydispersity index (PDI) measurements and ζ-potential analysis. The in vitro toxicity assessments were performed using hemolysis and MTT assays on the HS5 cell line. In vivo, acute oral toxicity was evaluated by using LD 50 assays in mice. Additionally, antioxidant activity was assessed through biochemical analysis of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and liver tissue catalase, malondialdehyde (MDA), and glutathione (GSH) levels. The results revealed that the liposomes exhibited a uniform and spherical morphology with suitable physicochemical properties for drug delivery applications. The in vitro cytotoxicity and hemolysis assays and the in vivo LD 50 experiment indicated the potential safety of γ oryzanol liposomes, especially at lower concentrations. In addition, the assessment of liver enzymes, i.e., ALT and AST, and the antioxidant markers further revealed the safety of the formulation, particularly for the liver as a highly sensitive soft organ. Overall, the liposome nanocarriers based on γ oryzanol were successfully formulated and expressed potential safety, supporting their application for the purposes of drug delivery and therapeutic interventions, particularly for hepatocellular and antioxidant therapies; however, further investigations for preclinical and clinical studies could be the future prospects for liposome nanocarriers based on γ oryzanol to explore the safety and efficacy of these nanocarriers in various disease models and clinical settings.

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Section: Methodsmentioning

confidence: 99%

Liposome Nanocarriers Based on γ Oryzanol: Preparation, Characterization, and In Vivo Assessment of Toxicity and Antioxidant Activity

Jasim,

Albukhaty,

Sulaiman

et al. 2024

ACS Omega

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“…After evaporation of the solvent, dried lipid films were hydrated by 20 mM Tris-HCl and 50 mM NaCl (TN buffer, pH 7.9) to a final lipid concentration of 5 mM. Small unilamellar vesicles (SUVs) were prepared by sonication of lipid suspension in cold water in a Branson M2800 ultrasonic bath sonicator for 20 minutes (Mendez, 2023), followed by passing them through 0.1 µm polycarbonate membranes (Avanti Polar Lipids) 13 times. SUVs can be stored at 4 °C for 7 days without freezing/thawing.…”

Section: Methodsmentioning

confidence: 99%

A packaging signal-binding protein regulates the assembly checkpoint of integrative filamentous phages

Yeh,

Feehley,

Feehley

et al. 2024

Preprint

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Many integrative filamentous phages not only lack Ff coliphage homologues essential for assembly but also have distinct packaging signals (PS). Their encapsidation remains completely uncharacterized to date. Here we report the first evidence of a PS-dependent checkpoint for integrative filamentous phage assembly. Suppressor screening of PS-deficient phages identified an unknown protein, PSB15 (PS-binding15kDa), crucial for encapsidation. The WAGFXF motif of the PSB15 N-terminus directly binds to PS DNA with conformational change, while suppressor mutations relieve DNA binding specificity constraints to rescue assembly arrest. PSB15 interacts with phospholipid cardiolipin via its basic helix and C-terminus, and recruits PS DNA to the inner membrane (IM). The PSB15-PS complex is released from the IM by interaction between its hydrophobic linker and thioredoxin (Trx), a host protein that is required for Ff assembly but whose mechanisms are still unclear. Live cell imaging shows that thioredoxin and DNA binding regulate the dwelling time of PSB15 at cell poles, suggesting that they both facilitate the dissociation of PSB15 from the IM. Loss of PSB15 or its PS-binding and IM-targeting/dissociation activity compromised virus egress, indicating that the PS/PSB15/Trx complex establishes a regulatory phage assembly checkpoint critical for integrative phage infection and life cycles.

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Cytotoxic activity of guanidinium copolymers loaded with silver nanoparticles and their interaction with model membranes

Gorbunova,

Efimova,

Grokhovskaya

et al. 2024

Colloid Polym Sci

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Sonication-Based Basic Protocol for Liposome Synthesis

Cited by 6 publications

References 24 publications

Liposome Nanocarriers Based on γ Oryzanol: Preparation, Characterization, and In Vivo Assessment of Toxicity and Antioxidant Activity

Liposome Nanocarriers Based on γ Oryzanol: Preparation, Characterization, and In Vivo Assessment of Toxicity and Antioxidant Activity

A packaging signal-binding protein regulates the assembly checkpoint of integrative filamentous phages

Cytotoxic activity of guanidinium copolymers loaded with silver nanoparticles and their interaction with model membranes

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