Sonication (Ultrasound) Affects In Vitro Growth of Hybrid Cymbidium

Abstract

Teixeira da Silva J.A., Dobránszki J., 2014: Sonication (ultrasound) The use of ultrasound or sonication has been shown to stimulate growth and development of several plant species. No study exists on the impact of sound on Cymbidium growth in vitro. Using sonication at 60 Hz for several time periods, the response on new protocorm-like body (neo-PLB) formation on Teixeira Cymbidium (TC) medium was examined, as was the response on in vitro plant organogenesis. Sonication for 5 or 10 min stimulated neo-PLB formation significantly more than plant growth regulator (PGR)-free TC medium without sonication (negative control) and more than 1, 20 or 45 min sonication, but significantly less than control TC medium containing PGRs NAA and Kin (positive control) after 60 days in culture. Sonication, when applied to PLBs, did not influence most plantlet-related growth parameters. Flow cytometric analyses registered an increase in endoreduplication in sonicated PLB tissues. Sonication at 60 Hz has a PLB-promoting effect, but is not as effective as PGRs.

mRNA transcription profile of potato (Solanum tuberosum L.) exposed to ultrasound during different stages of in vitro plantlet development

“…), even though sonication at 1 kHz and 100 dB did not induce changes in DNA content, there was a significant increase in the RNA and protein content (Shao et al 2008; Wang et al 2003), which indicates altered gene expression in response to sound waves. When hybrid Cymbidium Twilight Moon ‘Day Light’ protocorm-like bodies (PLBs) were irradiated by sound waves (60 Hz, for 5 and 10 min), there was an increase in transient endopolyploidy while PLB growth and the conversion to plantlets improved (Teixeira da Silva and Dobránszki 2014b). When Jeong et al (2008) used a sound-treated subtractive library and mRNA expression analyses (Northern blots and/or RT-PCR) to assess rice ( Oryza sativa L. ‘Donggin’), they detected four genes, including the rbcS (ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit) and ald (aldolase) genes, both of which were responsive to sound.…”

In Vitro Indirect Somatic Embryogenesis and Secondary Metabolites Production in the Saffron: Emphasis on Ultrasound and Plant Growth Regulators

“…Low energy ultrasound (US) acts as a physical stress, which influence the biological, biochemical and developmental processes of the plant cells (Teixeira da Silva & Dobranszki 2014). Cavitation and acoustic micro streaming produced by lowfrequency ultrasound cause the modifications in the cellular ultrastructure, cell membrane permeability, enzyme permanency and finally cell development progress (Ward et al 2000;Guzman et al 2001).…”

Improving the stage of rhizogenesis in clonal microropagation of clemat

“…Анализ литературных источников показывает, что ультразвуковая обработка вызывает изменения проницаемости, эластичности и вязкости клеточных мембран, транспорта через мембраны, а также конформации и активности мембраносвязанных ферментов и ферментов, участвующих в гормональной регуляции и ответе на стрессовые воздействия путём запуска системы антиоксидантной защиты [14,17,19]. У проростков, облученных in vitro ультразвуковыми волнами, может наблюдаться усиленный рост или может начаться органогенез, но реакция зависит от вида растений, а также от частоты и периода воздействия УЗИ [16,18]. В литературе есть сведения об успешном применении УЗ для стимуляции процесса образования корней как у одревесневших и зелёных черенков [2,10], так и у микрочеренков в стерильных условиях [9].…”