Sonochemical-solvothermal synthesis of guanine embedded copper based metal-organic framework (MOF) and its effect on oprD gene expression in clinical and standard strains of Pseudomonas aeruginosa

Abstract

The guanine incropped Cu based metal-organic framework (Guanine-Cu-MOF) was synthesized by facile one-step sonochemical method by simply mixing of 4-4, biphenyldicarboxylic, guanine and copper nitrate (Bio-Cu-Hbpdc-Gu). The prepared guanine-MOF was characterized by using X-Ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and Field emission scanning electron microscopy (FE-SEM) techniques. The morphology of prepared material was sponge-shaped which it was well documented, together with the presence of existing functional groups. The effect of prepared material on oprD Gene Expression was investigated in Clinical and Standard Strains of Pseudomonas aeruginosa (PAO-1) and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of prepared samples against P. aeruginosa strains were determined through the broth micro-dilution method. The expression of oprD gene in strains affected by Cu-Hbpdc-Gu was quantitatively investigated through real-time PCR. MIC of Bio-Cu-Hbpdc-Gu was 400 μg/mL for the standard and clinical strains of P. aeruginosa, while, MBC of this compound was 700 μg/mL for standard strain and 800 μg/mL for clinical strains. The highest and the lowest rate of oprD gene expression were found to be 3.6 and 1.1 fold in the strains, respectively.

RETRACTED: Taguchi-Assisted Optimization Technique and Density Functional Theory for Green Synthesis of a Novel Cu-MOF Derived From Caffeic Acid and Its Anticancerious Activities

“…The bactericidal mechanism of Cu-MOF is due to the diffusion of Cu 2+ ions. Also, these positive ions are absorbed by the negatively charged lipoproteins in the bacterial cell wall, enter the cell and damage the cell wall, alter its enzymatic function, or create holes in the cell wall (Abbasloo et al, 2018).…”

Hydrothermal Synthesis of Humate‐Layer‐Based Bimetal Organic Framework Composites as High Rate‐Capability and Enery‐Density Electrode Materials for Supercapacitors

“…In the FTIR spectra, the HA‐NiCo‐BPDC (1 : 1) has a couple of very strong absorption peaks at ∼1595 cm −1 and ∼1377 cm −1 similar to the HA−Na and NiCo‐BPDC, assigned to asymmetric and symmetric stretching vibrations of carboxylates from the BPDC and humates. The composites also exhibit a group of absorption peaks closely at 1181 cm −1 , 1104 cm −1 , 819 cm −1 , 762 cm −1 and 681 cm −1 similar to the NiCo‐BPDC, belonging to the typical absorption of aromatic rings . In addition, the composites own two peaks at ∼443 cm −1 and ∼528 cm −1 associated with the Ni−O and Co−O stretching vibration, indicating that carboxylate ions in humates or BPDC have bonded with nickel ions or cobalt ions to form carboxylates.…”

Porous Cu-MOF Nanostructures with Anticancer Properties Prepared by a Controllable Ultrasound-Assisted Reverse Micelle Method

“…[19,22] The C=O vibration in Cu-MOF is responsible for the peaks ranging from 2355 cm −1 to 2099 cm −1 and 1632 cm −1 . [16] and COO as CA vibration in Cu-MOF [23], respectively. The strong absorption peaks at 1095, 1152 and 1632 cm −1 corresponds to the asymmetric and symmetric C-O stretching [24,25].…”

“…In addition, the negative charges of lipoproteins are absorbed into the cell wall, they enter the cell and damage the cell wall, alter the function of the enzymes of the cell wall, or create cell wall holes. [16] In this paper, we have reported synthesis of Cu-MOF using ultrasonic assisted reverse micelle method and characterized by FT-IR, XRD, SEM and nitrogen adsorption. Anticancer activities against MCF-7 breast cancer cells are also reported.…”