Sonoclot evaluation of whole blood coagulation in healthy adult dogs

Babski, Danielle M.; Brainard, Benjamin M.; Krimer, Paula M.; Ralph, Alan; Pittman, Jennifer R.; Koenig, Amie

doi:10.1111/j.1476-4431.2012.00820.x

Cited by 18 publications

(31 citation statements)

References 18 publications

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“…ACT and CR values were obtained at every time point for all dogs, although timing necessitated the termination of some runs before the PF could be calculated. Baseline ACT and CR fell within the institutional reference interval (ACT 56.0–154.0 seconds, CR 14.9–46.0) at hour 0 on day 1 except for 1 dog with a slightly steeper clot rate (52). The final sample for all dogs on day 4 also fell within the institutional reference interval.…”

Section: Resultsmentioning

confidence: 88%

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Sonoclot® Evaluation of Single‐ and Multiple‐Dose Subcutaneous Unfractionated Heparin Therapy in Healthy Adult Dogs

Babski

Brainard

Ralph

et al. 2012

Veterinary Internal Medicne

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Background: Heparin therapy is difficult to monitor due to variation in animal response. While laboratory measurements of activated partial thromboplasin time (aPTT) and Anti-Xa activity (AXA) accurately describe heparin effect, their availability is limited.Hypothesis: Sonoclot analysis would be as sensitive as AXA and aPTT to monitor effects of unfractionated heparin (UFH) in healthy adult dogs.Animals: Six adult mixed-breed dogs. Methods: A prospective study design was employed. On day 1, baseline samples were collected (CBC, PT, aPTT, and Sonoclot), and UFH (300 U/kg SC) was administered to 6 dogs following an IV loading dose of 50 U/kg. Sonoclot and aPTT were performed hourly for 12 hours. AXA was assayed at hours 3, 6, 9, and 12. UFH (300 U/kg q8 h SC) was administered at 12 hours, and subsequently (q8 h) for 2 additional days. On day 4, a final dose of UFH was administered, and a sampling protocol identical to day 1 was performed.Results: Sonoclot activated clotting time (ACT) and clot rate (CR) correlated with AXA (R = 0.69, R = 0.65, respectively, P < .001), although to a lesser degree than aPTT (R = 0.75, P < .001). Linear regression using ACT and CR as covariates indicated a stronger correlation with AXA (R = 0.73, P < .001). ACT values strongly correlated with aPTT (R = 0.87, P < .001).Conclusions and Clinical Importance: Administration of UFH to healthy dogs results in progressive changes in Sonoclot values. AXA was correlated with a combination of ACT and CR and with aPTT. Sonoclot may play a role in monitoring UFH therapy; however, prospective studies evaluating its utility in clinical cases are warranted.

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Section: Resultsmentioning

confidence: 88%

“…The Sonoclot analyzer provides information on the entire hemostasis process in both a qualitative graph (Sonoclot Signature) and as quantitative results: the activated clotting time (ACT), the clot rate (CR), and the platelet function (PF) . Reference intervals for the sonoclot parameters in healthy adult dogs are available …”

mentioning

confidence: 99%

Sonoclot® Evaluation of Single‐ and Multiple‐Dose Subcutaneous Unfractionated Heparin Therapy in Healthy Adult Dogs

Babski

Brainard

Ralph

et al. 2012

Veterinary Internal Medicne

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“…17 Briefly, 20 µL of 0.2M CaCl 2 j was added to each cuvette, and the solution was warmed to 37°C. 17 Briefly, 20 µL of 0.2M CaCl 2 j was added to each cuvette, and the solution was warmed to 37°C.…”

Section: Methodsmentioning

confidence: 99%

Viscoelastic pharmacodynamics after dalteparin administration to healthy dogs

Brainard¹,

Koenig²,

Babski³

et al. 2012

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“…Using such a complex scoring system, groups with low (15%) and high mortality (40%) have been discriminated [20]. Previous studies have suggested that a combination of the APACHE II and ISTH scores predict mortality in critically ill patients better than the APACHE II score alone [21].…”

Section: Discussionmentioning

confidence: 99%

“…4 mg/l, respectively [6]. The diagnostic values for ACT (119-195 s), clot rate (7-23 sig/min), and platelet function (!1.5) were therefore chosen as discriminating values to separate patients with normal and pathological Sonoclot findings [10,20]. All laboratory tests to confirm or reject the diagnosis of overt DIC were consecutively measured when DIC was clinically suspected on the basis of the symptoms of the patient or on laboratory data.…”

Section: Definitions and Treatmentmentioning

confidence: 99%

Sonoclot coagulation analysis

Wan

Qian

et al. 2016

Blood Coagulation &Amp; Fibrinolysis

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Disseminated intravascular coagulation (DIC) contributes to high mortality. The study was performed to investigate Sonoclot as a potential predictor of 30-day survival in overt DIC. This cohort included 237 consecutive critically ill patients with overt DIC, admitted to a 15-bed multidisciplinary ICU between July 2010 and July 2013. Hemostasis was analyzed with Sonoclot, including activated clotting time (ACT), clot rate, and platelet function, as well as routine clotting test at admission to the critical care center. Sonoclot variables differed in survivors and nonsurvivors. Mean ACT was prolonged (289.9 ± 200.5 vs. 194.8 ± 126.6 s; P < 0.001) and platelet function (1.2 ± 0.9 vs. 1.6 ± 1.2; P = 0.010) was reduced in nonsurvivors. The clot rate was not different. Cox proportional-hazard model showed that ACT and platelet function correlated independently with survival (P < 0.05). Kaplan-Meier survival curve analysis suggested that patients with one pathological Sonoclot findings have better outcome (P < 0.05). After ACT and platelet function were introduced, the receiver-operating characteristic area under the curve of model achieved 0.876 (P < 0.05), with a specificity of 82.6% and a sensitivity of 80.5% in prediction of 30-day survival by multivariate analyses. Our data suggest that the Sonoclot can predict mortality in critically ill patients with overt DIC.

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Sonoclot evaluation of whole blood coagulation in healthy adult dogs

Abstract: Sonoclot provides viscoelastic evaluation of canine whole blood coagulation and correlated to several TEG parameters and fibrinogen. A standard protocol and reference intervals were established.

Cited by 18 publications

References 18 publications

Sonoclot® Evaluation of Single‐ and Multiple‐Dose Subcutaneous Unfractionated Heparin Therapy in Healthy Adult Dogs

Sonoclot® Evaluation of Single‐ and Multiple‐Dose Subcutaneous Unfractionated Heparin Therapy in Healthy Adult Dogs

Viscoelastic pharmacodynamics after dalteparin administration to healthy dogs

Sonoclot coagulation analysis

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