Sonolysis of Escherichia coli and Pichia pastoris in microfluidics

Tandiono, Tandiono; Ow, Dave Siak-Wei; Driessen, Leonie M. A.; Chin, Cara Sze-Hui; Klaseboer, Evert; Choo, Andre; Ohl, Siew-Wan; Ohl, Claus‐Dieter

doi:10.1039/c2lc20861j

Cited by 56 publications

(53 citation statements)

References 27 publications

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“…The results indicate that some flows created by oscillating bubbles in narrow gaps may not be accurately described with inviscid potential flow in cylindrical symmetry. Particularly, in studying the mixing/emulsification of flows or the deformation of elastic objects, such as yeast cells [35], it is necessary to account for the vorticity generation and transport. For both applications, it would be interesting to extend the present simulation with two-way coupling.…”

Section: Discussionmentioning

confidence: 99%

Shearing flow from transient bubble oscillations in narrow gaps

Mohammadzadeh

Ohl

2017

Phys. Rev. Fluids

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The flow driven by a rapidly expanding and collapsing cavitation bubble in a narrow cylindrical gap is studied with the volume of fluid method. The simulations reveal a developing plug flow during the early expansion followed by flow reversal at later stages. An adverse pressure gradient leads to boundary layer separation and flow reversal, causing large shear stress near the boundaries. Analytical solution to a planar pulsating flow shows qualitative agreement with the CFD results. The shear stress close to boundaries has implications to deformable objects located near the bubble: experiments reveal that thin, flat biological cells entrained in the boundary layer become stretched, while cells with a larger cross-section are mainly transported with the flow.

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Section: Discussionmentioning

confidence: 99%

Shearing flow from transient bubble oscillations in narrow gaps

Mohammadzadeh

Ohl

2017

Phys. Rev. Fluids

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“…The surface waves created facilitate the generation of strongly oscillating cavitation bubbles and even sonoluminiscence [76]. The developed platform can potentially be used to harvest cell contents, for cell lysis or other treatments [77]. The development of HIFU for microfluidic systems is still at a very early stage though.…”

Section: Bubble Dynamics In Bioapplications Achievements and Challenmentioning

confidence: 99%

Bubbles with shock waves and ultrasound: a review

2015

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The study of the interaction of bubbles with shock waves and ultrasound is sometimes termed ‘acoustic cavitation'. It is of importance in many biomedical applications where sound waves are applied. The use of shock waves and ultrasound in medical treatments is appealing because of their non-invasiveness. In this review, we present a variety of acoustics–bubble interactions, with a focus on shock wave–bubble interaction and bubble cloud phenomena. The dynamics of a single spherically oscillating bubble is rather well understood. However, when there is a nearby surface, the bubble often collapses non-spherically with a high-speed jet. The direction of the jet depends on the ‘resistance' of the boundary: the bubble jets towards a rigid boundary, splits up near an elastic boundary, and jets away from a free surface. The presence of a shock wave complicates the bubble dynamics further. We shall discuss both experimental studies using high-speed photography and numerical simulations involving shock wave–bubble interaction. In biomedical applications, instead of a single bubble, often clouds of bubbles appear (consisting of many individual bubbles). The dynamics of such a bubble cloud is even more complex. We shall show some of the phenomena observed in a high-intensity focused ultrasound (HIFU) field. The nonlinear nature of the sound field and the complex inter-bubble interaction in a cloud present challenges to a comprehensive understanding of the physics of the bubble cloud in HIFU. We conclude the article with some comments on the challenges ahead.

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“…A piezo transducer was attached to the top of the glass slide adjacent to the microchannels, after which the acoustic pressure on the glass plate was measured. 10 Next, MMBs were injected into the microfluidic channel using a syringe. Upon sweeping the ultrasonic frequency from 10 to 1000 kHz through a function generator, the resulting MMB oscillations were recorded using a high-speed camera (Photron SA1.1, Brookfield, CT, USA) at 100 000 frames per second.…”

Section: Measurement Of Mmb Resonant Frequenciesmentioning

confidence: 99%

Controlled nanoparticle release from stable magnetic microbubble oscillations

Gao

Chan

et al. 2016

NPG Asia Mater

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Magnetic microbubbles (MMBs) are microbubbles (MBs) coated with magnetic nanoparticles (NPs). MMBs not only maintain the acoustic properties of MBs, but also serve as an important contrast agent for magnetic resonance imaging. Such dual-modality functionality makes MMBs particularly useful for a wide range of biomedical applications, such as localized drug/gene delivery. This article reports the ability of MMBs to release their particle cargo on demand under stable oscillation. When stimulated by ultrasound at resonant frequencies, MMBs of 450 nm to 200 μm oscillate in volume and surface modes. Above an oscillation threshold, NPs are released from the MMB shell and can travel hundreds of micrometers from the surface of the bubble. The migration of NPs from MMBs can be described with a force balance model. With this technology, we deliver doxorubicincontaining poly(lactic-co-glycolic acid) particles across a physiological barrier both in vitro and in vivo, with a 18-fold and 5-fold increase in NP delivery to the heart tissue of zebrafish and tumor tissue of mouse, respectively. The penetration of released NPs in tissues is also improved. The ability to remotely control the release of NPs from MMBs suggests opportunities for targeted drug delivery through/into tissues that are not easily diffused through or penetrated. NPG Asia Materials (2016) 8, e260; doi:10.1038/am.2016.37; published online 8 April 2016 INTRODUCTIONTargeted drug delivery and controlled release is the 'holy grail' of nanomedicine. In this regard, one emerging strategy employs a localized stimulus to precisely deposit drug-containing nanoparticles (NPs) at the tissue/organ of interest. The deposited NPs then continuously release drug molecules in a localized and sustained manner. Current strategies trigger the release of NPs from carriers via enzymes, 1 magnetic fields 2 and shear stress. 3 However, these methods are limited to specific organs/tissues, such as blocked blood vessels. A more versatile technology is desired that allows the targeted delivery of NPs to any organ/tissue while maintaining traceability using a non-invasive imaging modality.The present paper reports a strategy toward this end. Magnetic microbubbles (MMBs), the combination of multi-modal contrastenhanced imaging (both magnetic resonance imaging and ultrasound imaging) with targeted drug delivery (magnetic targeting) and controlled release (bubble cavitation) at any site of interest represents an emerging theranostic platform. However, the current formulations of MMBs, stabilized by a polymeric, lipid or silica shell, need a high acoustic energy field to trigger the release through bubble

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Sonolysis of Escherichia coli and Pichia pastoris in microfluidics

Cited by 56 publications

References 27 publications

Shearing flow from transient bubble oscillations in narrow gaps

Shearing flow from transient bubble oscillations in narrow gaps

Bubbles with shock waves and ultrasound: a review

Controlled nanoparticle release from stable magnetic microbubble oscillations

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