Sonoporation of the Minicircle-VEGF165 for Wound Healing of Diabetic Mice

Yoon, Chang Shin; Jung, Hwa Jin; Kwon, Min Jeong; Lee, S. H.; Kim, C. W.; Kim, M. K.; Lee, M.; Park, J. H.

doi:10.1007/s11095-008-9778-x

Cited by 48 publications

(27 citation statements)

References 42 publications

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“…It is produced in Escherichia coli by att site-specific recombination catalyzed by the phage ⌽31 integrase (15). Minicircle DNA has great advantages over conventional DNA vectors for biosafety and robust and persistent gene expression, which have been demonstrated in muscle, liver, heart, human carcinoma xenograft tumors, and iPS cells (16)(17)(18)(19)(20)(21). The unique feature of minicircle DNA to enhance levels and duration of protein expression allows us to investigate whether minicircle DNA functions as an innovative vaccine delivery platform.…”

mentioning

confidence: 99%

In VivoElectroporation of Minicircle DNA as a Novel Method of Vaccine Delivery To Enhance HIV-1-Specific Immune Responses

Wang

Jiang

Chen

et al. 2014

J Virol

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mentioning

confidence: 99%

In VivoElectroporation of Minicircle DNA as a Novel Method of Vaccine Delivery To Enhance HIV-1-Specific Immune Responses

Wang

Jiang

Chen

et al. 2014

J Virol

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“…Intramuscular injection of a plasmid coding for VEGF prevented the development of diabetic neuropathy in rodents and rabbits (Yoon et al, 2009). VEGF was previously shown to display neuroprotective effects following ischemia, suggesting that VEGF may be applied as a B.N.…”

Section: Discussionmentioning

confidence: 98%

Construction and identification of pIRES2-NGF-VEGF165 bicistronic eukaryotic expression vector

Hong

et al. 2014

Genet. Mol. Res.

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ABSTRACT. We used a simple and efficient method to construct the bicistronic eukaryotic expression vector pIRES2-NGF-VEGF 165 . The nerve growth factor (NGF) gene was obtained from the genomic DNA of human peripheral blood mononuclear cells by polymerase chain reaction. The NGF cDNA fragment was inserted into the multiple cloning sites of the pIRES2-EGFP vector to generate the bicistronic eukaryotic expression plasmid pIRES2-NGF-EGFP. The vascular endothelial growth factor 165 (VEGF 165 ) gene was obtained from the pIRES2-VEGF 165 -EGFP plasmid by polymerase chain reaction. Next, the VEGF 165 cDNA fragment was cloned into pIRES2-NGF-EGFP in place of enhanced green fluorescent protein creating the plasmid pIRES2-NGF-VEGF 165 . pIRES2-NGF-VEGF 165 was transfected into HEK293 cells and reverse transcriptionpolymerase chain reaction and Western blot analysis were used to test the co-expression of double genes. The NGF and VEGF 165 genes were cloned and the DNA was sequenced, which revealed that NGF and VEGF 165 were consistent with the sequence recorded in GenBank. Restriction analysis showed that the NGF and VEGF 165 genes were inserted into the expression vector pIRES2-EGFP. Transfection of pIRES2-NGF- 5675©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 5674-5685 (2014) Construction and identification of pIRES2-NGF-VEGF 165 VEGF 165 into HEK293 cells resulted in expression of the double gene at the mRNA and protein levels. The NGF and VEGF 165 coexpression plasmid provides a novel expression system, enabling further study of the functions of the NGF and VEGF 165 genes.

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“…Intramuscular injection of a plasmid coding for VEGF prevents the development of diabetic neuropathy in rodents and rabbits (Yoon et al, 2009). VEGF was previously shown to have neuroprotective effects following ischemia, suggesting that VEGF may be applied as a neuroprotective agent for treating other neurological diseases (Fujiki et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Construction and identification of pIRES2-VEGF165-NT-3 bicistronic eukaryotic expression vector

Li¹,

Li²,

Feng³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. We used a simple and efficient method to construct the bicistronic eukaryotic expression vector pIRES 2 -VEGF 165 -NT-3. The neurotrophin-3 (NT-3) gene was obtained from the genomic DNA of human peripheral blood mononuclear cells by polymerase chain reaction. The NT-3 cDNA fragment was cloned into the pIRES 2 -VEGF 165 -EGFP vector in place of enhanced green fluorescent protein (EGFP) to create the plasmid pIRES 2 -VEGF 165 -NT-3. Next, pIRES 2 -VEGF 165 -NT-3 was transfected into HEK293 cells, and reverse transcription-polymerase chain reaction and Western blotting were used to test co-expression of the double genes. The vascular endothelial growth factor 165 (VEGF 165 ) and NT-3 genes were cloned; DNA sequencing analysis demonstrated that the VEGF 165 and NT-3 sequences were the same as those recorded in GenBank. Restriction analysis indicated that the VEGF 165 and NT-3 genes were correctly inserted into the expression vector pIRES 2 -EGFP. The double gene was expressed at both the mRNA and protein levels. The VEGF 165 and NT-3 co-expression plasmid was successfully constructed, providing a novel expression system for further study of the functions of the VEGF 165 and NT-3 genes.

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Sonoporation of the Minicircle-VEGF165 for Wound Healing of Diabetic Mice

Abstract: Ultrasound mediated gene therapy with the minicircle-VEGF(165) is effective for the healing of the skin wound of the diabetic mice.

Cited by 48 publications

References 42 publications

In VivoElectroporation of Minicircle DNA as a Novel Method of Vaccine Delivery To Enhance HIV-1-Specific Immune Responses

In VivoElectroporation of Minicircle DNA as a Novel Method of Vaccine Delivery To Enhance HIV-1-Specific Immune Responses

Construction and identification of pIRES2-NGF-VEGF165 bicistronic eukaryotic expression vector

Construction and identification of pIRES2-VEGF165-NT-3 bicistronic eukaryotic expression vector

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