Sonoprinting nanoparticles on cellular spheroids via surface acoustic waves for enhanced nanotherapeutics delivery

Abstract

In this paper, we introduce a SAW-based platform that generates acoustic microstreaming and Bjerknes forces to enhance the delivery of nanoparticles on the spheroid's surface and improve their penetration into deeper layers of spheroids.

Three-dimensional array of microbubbles sonoporation of cells in microfluidics

“…Moreover, the microbubble generated at the air/liquid interface without employing a shell is capable of oscillating freely, enhancing the efficiency of sonoporation. Recent studies on sonoporation in combination with the acoustic streaming approach suggest that acoustic streaming plays a large role in enhancing cell membrane permeability Salari et al (2021); Zhao et al (2021); Rasouli et al (2023). Therefore, the acoustic streaming generated by the oscillation of three-dimensional array of microbubbles can Cell staining experiments were performed on cells in the presence of microbubbles, ultrasound, and microbubble + ultrasound.…”

“…Viral vector methods are associated with cytotoxicity, while cationic lipids do not offer uniform and dosage-controlled delivery across within a cell population Lundstrom (2018) ; Carugo et al (2015) ; Stewart et al (2018) . Physical methods primarily involve transiently perforating the cell membrane using acoustic, optical, electrical, and mechanical methods to facilitate the intracellular delivery of exogenous cargoes Morshedi Rad et al (2021) ; Chakrabarty et al (2022) ; Rasouli et al (2023) ; Wang and Lu (2006) ; Uvizl et al (2021) . Of the physical permeabilization approaches, sonoporation wields great potential and has been demonstrated as an efficacious technology for delivering a variety of functional cargos to different types of cells Azmin et al (2012) ; Wu et al (2006) ; Rich et al (2022) .…”