Sophisticated Regulation of Transcriptional Factors by the Bacterial Phosphoenolpyruvate: Sugar Phosphotransferase System

Galinier, Anne; Deutscher, Josef

doi:10.1016/j.jmb.2017.02.006

Cited by 87 publications

(71 citation statements)

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“…The results of the GO enrichment analysis implied that phosphotransferase systems that were activated in WT earlier were now repressed, suggesting that these functions were more important in the early than in the midexponential phase. PTS are known to work not only as carbohydrate transport and modification systems in all phyla of bacteria, but also have regulatory roles in numerous cellular functions (42). In E. coli, glucose-specific PTS was found to inhibit other non-PTS sugar permeases influencing the availability of glucose via dephosphorylation of the EIIA subunit (43).…”

Section: Functional Distribution Of Qs-regulated Genesmentioning

confidence: 99%

Characterisation of Quorum Sensing System and Its Role in Global Regulation in Hafnia alvei

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Quorum sensing (QS) is a regulatory process achieved via cell-to-cell communication that involves release and detection of autoinducers (AIs), and which occurs in a wide range of bacteria. To date, QS has been associated with events of pathogenesis, biofilm formation, and antibiotic resistance in clinical, industrial, and agricultural contexts. The main objective of this study was to characterise the role of N-Acyl homoserine lactone (AHL) type QS in Hafnia alvei FB1, a bacterial strain isolated from frozen vacuum-packed fish paste meatballs, via identification of QS core genes using a genomic approach, followed by comparative transcriptomic profiling between QS-deficient mutants and wild-type strains. H. alvei FB1 is known to produce two types of AHLs, namely, N-(3-oxohexanoyl) homoserine lactone (3OC6-HSL) and N-(3-oxooctanoyl) homoserine lactone (3OC8-HSL). The complete genome sequence of strain FB1 was obtained and a single gene for AHL synthase (halI) and its cognate receptor (halR) were identified. QS-deficient mutants of FB1 were constructed via the λ-Red recombineering method. Removal of the QS genes in strain FB1 affected mainly mechanisms in cell division and nutrient uptake, as well as resistance to a number of antibiotics, which are crucial for survival, adaptation and colonisation of both food and the host gut environment.Impact statement: Members of the genus Hafnia are known as opportunistic pathogens in both nosocomial and community-acquired infections. However, the involvement and mechanism of pathogenesis of Hafnia in infectious diseases remains uncertain. We investigatd the role of the signalling molecule group, N-acyl homoserine lactones (AHLs), in a Hafnia alvei strain, since AHLs play important roles in pathogenicity, survival or adaptation in other pathogens. The comparative transcriptomic study revealed that AHLs are involved in mechanisms in cell division and nutrient uptake, as well as resistance to a number of antibiotics, which are crucial for survival, adaptation and colonisation of both food and the host gut environment. These findings provide insight into possible strategies to combat this opportunistic pathogen.

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Section: Functional Distribution Of Qs-regulated Genesmentioning

confidence: 99%

Characterisation of Quorum Sensing System and Its Role in Global Regulation in Hafnia alvei

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“…This makes the physiological signalling of the PTS Ntr initiated in EI Ntr to be altogether overcome by the flow of P that pervades the system in the presence of fructose (Pfluger and de Lorenzo, 2008;Chavarria et al, 2013). And third, in most cases where EIIA Ntr of P. putida (Pfluger-Grau et al, 2011) or other bacteria (Muriel-Millan et al, 2015;Galinier and Deutscher, 2017) has been shown to inhibit A B Fig. 1.…”

Section: Introductionmentioning

confidence: 99%

“…This makes the physiological signalling of the PTS Ntr initiated in EI Ntr to be altogether overcome by the flow of ∼P that pervades the system in the presence of fructose (Pfluger and de Lorenzo, ; Chavarria et al, ). And third, in most cases where EIIA Ntr of P. putida (Pfluger‐Grau et al, ) or other bacteria (Muriel‐Millan et al, ; Galinier and Deutscher, ) has been shown to inhibit a biological activity through direct protein–protein interactions, the inhibitory species has been found to be the non‐phosphorylated one (there are some exceptions, though e.g. Yoo et al, ).…”

Section: Introductionmentioning

confidence: 99%

The interplay of EIIA^Ntr with C‐source regulation of the Pu promoter of Pseudomonas putida mt‐2

Pérez‐Pantoja

Kim

Platero

2018

Environmental Microbiology

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The presence of some sugars (e.g. glucose) downregulates the activity of the Pu promoter of plasmid pWW0 of Pseudomonas putida mt-2, which drives the upper TOL operon for biodegradation of m-xylene. Genetic evidence produced 20 years ago documented an effect of the EIIA (PtsN) protein of the nitrogen-related phosphoenolpyruvate-dependent phosphotransferase system (PTS ) in such a C-source control of Pu activity. In this study, we have exploited the wealth of recent information on the PTS of P. putida as well as transcriptomic data available in the last few years on this bacterium to revisit this question - and the role of EIIA as such. To this end, we examined Pu output under physiological conditions known to either phosphorylate PTS proteins to saturation or to deplete them altogether from high-energy phosphate. The results showed that Pu activity is checked by EIIA regardless of its phosphorylation state. However, such inhibition is intensified during growth on glucose (which correlates with more phosphate-free EIIA ) and partially relieved in fructose, which triggers phosphorylation of PTS proteins. These data explain former inconsistencies on the Pu-PTS interplay and provides a better understanding of the metabolic and regulatory retroactivity between the TOL plasmid and its host metabolism.

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“…337Moreover, in Gram positives, PTS systems have an important role in carbon catabolite repression via 338 phosphorylation cascades and direct interaction with the carbon catabolite repression protein A 339 (ccpA)(Galinier & Deutscher, 2017;Görke & Stülke, 2008). Heterofermentative LAB contain fewer 340 PTS system components than homofermentative LAB, which is thought to be the result of gene loss 341(Zheng, Ruan, Sun, & Gänzle, 2015).…”

mentioning

confidence: 99%

“…In general, organisms using the EMPP are believed to use PTS 342 systems, and organism using the PKP to use secondary carriers(Romano, Trifone, & Brustolon, 1979). 343Likely as a result of the lack of full PTS systems, glucose utilization is not constitutive but substrate-344 induced in heterofermenters, and utilization of several other sugars is not repressed by glucose 345(Galinier & Deutscher, 2017). Sugar transport in heterofermenters is poorly characterized, and only 346 recently a study was dedicated to the genomic and phenotypic characterization of carbohydrate 347 transport and metabolism in L. reuteri, as representative of heterofermentative LAB(Zhao & Gänzle, 348 2018).…”

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confidence: 99%

A metabolic reconstruction ofLactobacillus reuteriJCM 1112 and analysis of its potential as a cell factory

Kristjansdottir

Bosma

Santos

et al. 2019

Preprint

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BackgroundLactobacillus reuteri is a heterofermentative Lactic Acid Bacterium (LAB) that is commonly used for food fermentations and probiotic purposes. Due to its robust properties, it is also increasingly considered for use as a cell factory. It produces several industrially important compounds such as 1,3-propanediol and reuterin natively, but for cell factory purposes, developing improved strategies for engineering and fermentation optimization is crucial. Genome-scale metabolic models can be highly beneficial in guiding rational metabolic engineering. Reconstructing a reliable and a quantitatively accurate metabolic model requires extensive manual curation and incorporation of experimental data.ResultsA genome-scale metabolic model of L. reuteri JCM 1112T was reconstructed and the resulting model, Lreuteri_530, was validated and tested with experimental data. Several knowledge gaps in the metabolism were identified and resolved during this process, including presence/absence of glycolytic genes. Flux distribution between the two glycolytic pathways, the phosphoketolase and Embden-Meyerhof-Parnas pathways, varies considerably between LAB species and strains. As these pathways result in different energy yields, it is important to include strain-specific utilization of these pathways in the model. We determined experimentally that the Embden-Meyerhof-Parnas pathway carried at most 7% of the total glycolytic flux. Predicted growth rates from Lreuteri_530 were in good agreement with experimentally determined values. To further validate the prediction accuracy of Lreuteri_530, the predicted effects of glycerol addition and adhE gene knock-out, which results in impaired ethanol production, were compared to in vivo data. Examination of both growth rates and uptake- and secretion rates of the main metabolites in central metabolism demonstrated that the model was able to accurately predict the experimentally observed effects. Lastly, the potential of L. reuteri as a cell factory was investigated, resulting in a number of general metabolic engineering strategies.ConclusionWe have constructed a manually curated genome-scale metabolic model of L. reuteri JCM 1112T that has been experimentally parameterized and validated and can accurately predict metabolic behavior of this important platform cell factory.

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Sophisticated Regulation of Transcriptional Factors by the Bacterial Phosphoenolpyruvate: Sugar Phosphotransferase System

Cited by 87 publications

References 123 publications

Characterisation of Quorum Sensing System and Its Role in Global Regulation in Hafnia alvei

Characterisation of Quorum Sensing System and Its Role in Global Regulation in Hafnia alvei

The interplay of EIIA^Ntr with C‐source regulation of the Pu promoter of Pseudomonas putida mt‐2

A metabolic reconstruction ofLactobacillus reuteriJCM 1112 and analysis of its potential as a cell factory

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Sophisticated Regulation of Transcriptional Factors by the Bacterial Phosphoenolpyruvate: Sugar Phosphotransferase System

Cited by 87 publications

References 123 publications

Characterisation of Quorum Sensing System and Its Role in Global Regulation in Hafnia alvei

Characterisation of Quorum Sensing System and Its Role in Global Regulation in Hafnia alvei

The interplay of EIIANtr with C‐source regulation of the Pu promoter of Pseudomonas putida mt‐2

A metabolic reconstruction ofLactobacillus reuteriJCM 1112 and analysis of its potential as a cell factory

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The interplay of EIIA^Ntr with C‐source regulation of the Pu promoter of Pseudomonas putida mt‐2