Sophoranone, extracted from a traditional Chinese medicine <i>Shan Dou Gen,</i> induces apoptosis in human leukemia U937 cells via formation of reactive oxygen species and opening of mitochondrial permeability transition pores

Kajimoto, Sachiko; Takanashi, Noriko; Kajimoto, Tetsuya; Xu, Man; Cao, Judong; Masuda, Yutaka; Aiuchi, Toshihiro; Nakajo, Shigeo; Ida, Yoshiteru; Nakaya, Kazuyasu

doi:10.1002/ijc.10414

Cited by 62 publications

(39 citation statements)

References 52 publications

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“…This pattern of distinct DNA ladder is considered to be DOI:http://dx.doi.org/10.7314/APJCP.2014.15.16.6627 Fungal Taxol Extracted from Cladosporium oxysporum Induces Apoptosis in T47D Human Breast Cancer Cells a biochemical characteristic of apoptosis (Cohen, 1993). Similar anomalies had been observed earlier (Kajimoto et al, 2002;Alabsi et al, 2013).…”

Section: Discussionsupporting

confidence: 52%

Fungal Taxol Extracted from Cladosporium oxysporum Induces Apoptosis in T47D Human Breast Cancer Cell Line

Raj

Shanmugam²,

Manikanadan³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Purpose: The present study concerns molecular mechanisms involved in induction of apoptosis by a fungal taxol extracted from the fungus Cladosporium oxysporum in T47D human breast cancer cells. Materials and Methods: Apoptosis-induced by the fungal taxol was assessed by MTT assay, nuclear staining, DNA fragmentation, flow cytometry and pro-as well as anti-apoptotic protein expression by Western blotting. Results: Our results showed inhibition of T47D cell proliferation with an IC 50 value of 2.5 μM/ml after 24 h incubation. It was suggested that the extract may exert its anti-proliferative effect on human breast cancer cell line by suppressing growth, arresting through the cell cycle, increase in DNA fragmentation as well as down-regulation of the expression of NF-ĸB, Bcl-2 and Bcl-XL and up-regulation of pro-apoptotic proteins like Bax, cyt-C and caspase-3. Conclusions: We propose that the fungal taxol contributes to growth inhibition in the human breast cancer cell through apoptosis induction via a mitochondrial mediated pathway, with possible potential as an anticancer therapeutic agent.

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Section: Discussionsupporting

confidence: 52%

Fungal Taxol Extracted from Cladosporium oxysporum Induces Apoptosis in T47D Human Breast Cancer Cell Line

Raj

Shanmugam²,

Manikanadan³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Thus the cytotoxicity of flavonoids toward malignant cells has been examined. [28][29][30][31][32][33][34][35] On the other hand, there are many reports that flavonoids protect various cell types from oxida- tive stress. 11,12,36) Since oxidative stress is suggested to cause many diseases including cardiovascular and neurodegenerative diseases, 37) flavonoids are possible dietary preventives against these diseases.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of Flavonoids toward Cultured Normal Human Cells

Matsuo

Sasaki

Saga

et al. 2005

Biological & Pharmaceutical Bulletin

215

145

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Flavonoids are a group of plant polyphenols and are ubiquitously found in plants. 1,2) Fruits and vegetables, as well as popular beverages such as wine, tea, and coffee, are the main dietary sources of flavonoids. It has been reported that flavonoids show pharmacological effects such as antioxidant, 3,4) antiviral, 5) antitumor, 6) and antiinflammatory activities. 7) In particular, their antioxidant activity has attracted much attention as a possible dietary preventive against cardiovascular and neurodegenerative diseases. 6,8) There are many reports that flavonoids act as antioxidants and protect various cell types from oxidative stress-mediated cell injury. We reported that flavonoids have protective effects on human umbilical vein endothelial (HUVE) cells and rat neuronal cells (PC12) exposed to the highly toxic lipid peroxide linoleic acid hydroperoxide. [9][10][11] In addition, it has been observed that flavonoids suppress the cytotoxicity of hydrogen peroxide toward Chinese hamster cells (V79) 12) and oxidized low-density lipoproteins in human lymphoid cell lines, 13) glucose oxidase-mediated apoptosis in mouse thymocytes, 14) and metal-induced lipid hydroperoxide-dependent lipid peroxidation in a-linoleic acid-loaded rat hepatocytes. 15)On the other hand, it has been suggested that flavonoids act as mutagens, prooxidants, and enzyme inhibitors.16) Further, it has been reported that they exert cytotoxicity at higher concentrations and in the presence of oxidation-catalyzing factors such as transition metal ions. The cytotoxicity of flavonoids toward human promyelocytic leukemia cells (HL-60), 17) human acute myelogeneous leukemia cells (KG1, KG1a, THP-1, and U937), 18) rhesus monkey kidney cells (LLC-MK2), rat glial tumor cells (C6), 19) bovine leukemia virus-transformed lamb embryo kidney fibroblasts (FLK), 20) mouse and hamster pancreatic b-cells (b TC1 and HIT), 21) human fibroblasts (HFK-2), human keratinocytes (HaCaT), human breast cancer adenocarcinoma cells (MCF-7), human neuroblastoma cells (SHEP and WAC-2), and bovine capillary endothelial cells 22) has been found. As shown above, however, there are only a few reports on the cytotoxicity of flavonoids toward human normal cells.If flavonoids are used as dietary factors for health maintenance, relatively large amounts may be ingested. Thus the potentially toxic effects of excessive flavonoid intake must be clarified. 16) In the present paper, we describe the cytotoxicity of nine flavonoids, including the two flavones apigenin and luteolin, the three flavonols 3-hydroxyflavone, kaempherol, and quercetin, the flavonol glycoside rutin, the two flavanones eriodictyol and naringenin, and the flavanol taxifolin (Table 1 and Fig. 1), toward cultured normal human cells, TIG-1 cells and HUVE cells. Further, we examined the intracellular levels of ROS in flavonoid-treated TIG-1 cells using DCF-DA 23) and their incorporation of flavonoids in culture medium. The cytotoxicity of flavonoids, including apigenin, eriodictyol, 3-hydroxyflavone, kaempherol, luteolin,...

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“…Phytochemical investigations have revealed that the plant accumulated isoprenyl-substituted flavonoids and lupin alkaloids as its main constituents (Ding & Chen, 2006. Pharmacological studies showed that the isoprenylated flavanones isolated from this species could inhibit cell growth and induce apoptosis on various cell lines from human solid tumors and in human leukemia U937 cells (Kajimoto et al, 2002). Sophora moorcroftiana is an endemic shrub species in Tibet, China, and is mainly distributed in the wide valleys and the middle reaches of several main tributaries of Yalu Tsangbo River (Nianchu and Lhasa Rivers).…”

Section: Ethnopharmacological Usementioning

confidence: 99%

“…Sophoranone, extracted from Sophora flavescens induces apoptosis in human leukemia u937 cells Via formation of reactive oxygen species and opening of Mitochondrial permeability transition pores (Kajimoto et al, 2002).…”

Section: Induction Of Apoptosismentioning

confidence: 99%

A review on phytochemical, ethnomedical and pharmacological studies on genus Sophora, Fabaceae

Krishna¹,

Knv²,

Sandhya³

et al. 2012

Rev. bras. farmacogn.

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Sophora is a genus of the Fabaceae family, contains about 52 species, nineteen varieties, and seven forms that are widely distributed in Asia, Oceanica, and the Pacific islands, in the family Fabaceae of herbaceous (Sophora flavescens Aiton) to trees (Sophora japonica L.). More than fifteen species in this genus have a long history of use in traditional Chinese medicines. In the last decades the use of this genus in traditional Chinese drugs has led to rapid increase in the information available on active components and reported to posses various pharmacological/ therapeutic properties. The paper reviews the ethnopharmacology, the biological activities and the correlated chemical compounds of genus Sophora, Fabaceae. More than 300 compounds has been isolated, among them major are quinolizidine alkaloids particularly matrine and oxymatrine and flavonoids particularly prenylated and isoprenylated flavonoids. Modern pharmacological studies and clinical studies demonstrated that these chemical constituens possess wide reaching pharmacological actions like anti oxidant, anticancer, anti-asthamatic, anti-neoplastic, antimicrobial, antiviral, antidote, anti pyretic, cardiotonic, antinflammatory, diuretic and in the treatment of skin diseases like eczema, colitis and psoriasis.

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Sophoranone, extracted from a traditional Chinese medicine Shan Dou Gen, induces apoptosis in human leukemia U937 cells via formation of reactive oxygen species and opening of mitochondrial permeability transition pores

Cited by 62 publications

References 52 publications

Fungal Taxol Extracted from Cladosporium oxysporum Induces Apoptosis in T47D Human Breast Cancer Cell Line

Fungal Taxol Extracted from Cladosporium oxysporum Induces Apoptosis in T47D Human Breast Cancer Cell Line

Cytotoxicity of Flavonoids toward Cultured Normal Human Cells

A review on phytochemical, ethnomedical and pharmacological studies on genus Sophora, Fabaceae

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