Sorbitol induces flower bud formation via the MADS‐box transcription factor <i>EjCAL</i> in loquat

Xu, Huji; Meng, Dong; Chen, Ting; Qi, Meng; Li, Xiaoying; Ge, Hang

doi:10.1111/jipb.13439

Cited by 11 publications

(4 citation statements)

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“…The accumulation of carbohydrates in fruits is synergistically regulated by various TFs ( Ren et al., 2023 ). However, due to the delayed publication of the loquat genome and the absence of a stable gene function verification system, there have been few reports on TFs associated with the sugar metabolism pathway in loquat ( Xu et al., 2023 ). In this study, the comparison of protein sequences and promoter region of S6PDH ( EVM0006243 , EVM0044405 ) between TBY and TBW showed that there was no structural variation affecting gene function or expression, indicating that TFs may be responsible for the rapid up-regulation of S6PDH before maturation.…”

Section: Discussionmentioning

confidence: 99%

Multi-omics analysis provides new insights into the changes of important nutrients and fructose metabolism in loquat bud sport mutant

Song,

Zhao,

Pei

et al. 2024

Front. Plant Sci.

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Bud sport is a common and stable somatic variation in perennial fruit trees, and often leads to significant modification of fruit traits and affects the breeding value. To investigate the impact of bud sport on the main metabolites in the fruit of white-fleshed loquat, we conducted a multi-omics analysis of loquat fruits at different developmental stages of a white-fleshed bud sport mutant of Dongting loquat (TBW) and its wild type (TBY). The findings from the detection of main fruit quality indices and metabolites suggested that bud sport resulted in a reduction in the accumulation of carotenoids, fructose, titratable acid and terpenoids at the mature stage of TBW, while leading to the accumulation of flavonoids, phenolic acids, amino acids and lipids. The comparably low content of titratable acid further enhances the balanced and pleasent taste profile of TBW. Expression patterns of differentially expressed genes involved in fructose metabolism exhibited a significant increase in the expression level of S6PDH (EVM0006243, EVM0044405) prior to fruit maturation. The comparison of protein sequences and promoter region of S6PDH between TBY and TBW revealed no structural variations that would impact gene function or expression, indicating that transcription factors may be responsible for the rapid up-regulation of S6PDH before maturation. Furthermore, correlation analysis helped to construct a comprehensive regulatory network of fructose metabolism in loquat, including 23 transcription factors, six structural genes, and nine saccharides. Based on the regulatory network and existing studies, it could be inferred that transcription factors such as ERF, NAC, MYB, GRAS, and bZIP may promote fructose accumulation in loquat flesh by positively regulating S6PDH. These findings improve our understanding of the nutritional value and breeding potential of white-fleshed loquat bud sport mutant, as well as serve as a foundation for exploring the genes and transcription factors that regulate fructose metabolism in loquat.

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Section: Discussionmentioning

confidence: 99%

Multi-omics analysis provides new insights into the changes of important nutrients and fructose metabolism in loquat bud sport mutant

Song,

Zhao,

Pei

et al. 2024

Front. Plant Sci.

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“…The positive influence of exogenous hyperoside on flower buds indicates its great prospects in horticulture and agriculture applications. A recent study reported that sorbitol could promote the accumulation of hyperoside and ultimately prompt loquat flower bud differentiation through ERF12-CAL-GAT signaling pathway, again demonstrating the significant role of hyperoside in flower bud formation by potentially influencing downstream genes [ 41 ]. Therefore, it is of great significance to synthesize hyperoside in an efficient way.…”

Section: Discussionmentioning

confidence: 99%

The parallel biosynthesis routes of hyperoside from naringenin in Hypericum monogynum

Wang,

Cui,

et al. 2023

Horticulture Research

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Hyperoside is a bioactive flavonoid galactoside in both medicinal and edible plants. It plays an important physiological role in the growth of flower buds. However, the hyperoside biosynthesis pathway has not been systematically elucidated in plants including its original source Hypericaceae. Our group found abundant hyperoside in the flower buds of Hypericum monogynum, thereby we sequenced its transcriptome to study the biosynthetic mechanism of hyperoside. After gene screening and functional verification, four kinds of key enzymes were identified. Specifically, HmF3Hs and HmFLSs could catalyze flavanones into dihydroflavonols, as well as catalyzing dihydroflavonols into flavonols. HmFLSs could also convert flavanones into flavonols and flavones with varying efficiencies. HmF3′H was found to act broadly on 4′-hydroxyl flavonoids to produce 3′,4′-diydroxylated flavanones, dihydroflavonols, flavonols and flavones. And HmGAT would transform flavonols into the according 3-O-galactosides including hyperoside. The parallel hyperoside biosynthesis routes were thus depicted, one of which was successfully reconstructed in Escherichia coli BL21(DE3) by feeding economical naringenin, resulting in a hyperoside yield of 25 mg/L. Overall, this research not only helped us understand the interior catalytic mechanism of hyperoside in H. monogynum concerning flower development and bioactivity, but also provided valuable insights into these enzyme families.

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“…Generally, MADS-box genes are considered to operate as regulators of flower morphogenesis or pollen maturation ( Michaels and Amasino, 1999 ; Yu et al., 2014 ). For example, the loquat MADS-box genes EjCAL can promote the flower bud differentiation process ( Xu et al., 2022 ). The connection between MADS-box genes and fruit lignification could not be established until the loquat EjAGL65 was first identified ( Ge et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

The MADS-box gene EjAGL15 positively regulates lignin deposition in the flesh of loquat fruit during its storage

2023

Front. Plant Sci.

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IntroductionLignification of fruit flesh is a common physiological disorder that occurs during post-harvest storage, resulting in the deterioration of fruit quality. Lignin deposition in loquat fruit flesh occurs due to chilling injury or senescence, at temperatures around 0°C or 20°C, respectively. Despite extensive research on the molecular mechanisms underlying chilling-induced lignification, the key genes responsible for the lignification process during senescence in loquat fruit remain unknown. MADS-box genes, an evolutionarily conserved transcription factor family, have been suggested to play a role in regulating senescence. However, it is still unclear whether MADS-box genes can regulate the lignin deposition that arises from fruit senescence.MethodsBoth senescence- and chilling-induced flesh lignification were simulated by applying temperature treatments on loquat fruits. The flesh lignin content during the storage was measured. Transcriptomic, quantitative reverse transcription PCR and correlation analysis were employed to identify key MADS-box genes that may be involved in flesh lignification. The Dual-luciferase assay was utilized to identify the potential interactions between MADS-box members and genes in phenylpropanoid pathway. Results and DiscussionThe lignin content of the flesh samples treated at 20°C or 0°C increased during storage, but at different rates. Results from transcriptome analysis, quantitative reverse transcription PCR, and correlation analysis led us to identify a senescence-specific MADS-box gene, EjAGL15, which correlated positively with the variation in lignin content of loquat fruit. Luciferase assay results confirmed that EjAGL15 activated multiple lignin biosynthesis-related genes. Our findings suggest that EjAGL15 functions as a positive regulator of senescence-induced flesh lignification in loquat fruit.

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Sorbitol induces flower bud formation via the MADS‐box transcription factor EjCAL in loquat

Cited by 11 publications

References 64 publications

Multi-omics analysis provides new insights into the changes of important nutrients and fructose metabolism in loquat bud sport mutant

Multi-omics analysis provides new insights into the changes of important nutrients and fructose metabolism in loquat bud sport mutant

The parallel biosynthesis routes of hyperoside from naringenin in Hypericum monogynum

The MADS-box gene EjAGL15 positively regulates lignin deposition in the flesh of loquat fruit during its storage

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