Soroprevalência de anticorpos anti-Ehrlichia canis em cães de Cuiabá, Mato Grosso

Abstract

A erliquiose canina é uma doença transmitida por carrapatos Rhipicephalus sanguineus e ocasionada pela Ehrlichia canis, bactéria intracelular obrigatória. O presente estudo verificou a prevalência de anticorpos anti-E. canis em 254 cães de quatro regiões administrativas de Cuiabá, Estado de Mato Grosso, por imunofluorescência indireta, observando-se uma prevalência de 42,5% (108/254) sem diferença significativa entre as regiões. As variáveis idade, raça, sexo, hábitat, acesso à zona rural e presença de carrapatos foram analisadas. Os títulos de anticorpos variaram entre 1:40 a 1:2.560. Somente 32 (29,63%) cães soropositivos estavam infestados por carrapatos, todos R. sanguineus. O resultado encontrado confirma que não há predisposição racial, sexual ou etária, enquanto a menor ocorrência de cães reagentes no intradomicílio provavelmente está relacionada à baixa infestação por carrapato, apesar de não ter sido observada diferença significativa entre os cães com ou sem a infestação com o carrapato vetor.Palavras-chave: Ehrlichia canis, imunofluorescência indireta, Cuiabá, inquérito epidemiológico. AbstractCanine ehrlichiosis is a disease transmitted by ticks Rhipicephalus sanguineus and caused by Ehrlichia canis, obligatory intracellular bacteria. The present study examined the prevalence of anti-E. canis in 254 dogs from four administrative regions of Cuiabá, Mato Grosso, by indirect immunofluorescence assay. There was a prevalence of 42.5% (108/254) without significant difference between the studied regions. The variables age, breed, sex, habitat, access to rural and ticks were analyzed. The antibody titers ranged from 1:40 to 1:2,560. Only 32 (29.63%) seropositive dogs were infested with ticks, all R. sanguineus. The results confirm that do not have breed, sex or age predisposition to ehrlichiosis due E. canis, while the lowest occurrence of reactive dogs indoors probably related to low tick infestation, although no significant difference between dogs with or without infestation with the tick vector.

Canine ehrlichiosis: prevalence and epidemiology in northeast Brazil

“…However, On IFA, the seroprevalence (32.7%) found in this study was similar to the 35.6% prevalence reported by Souza et al (2010). However, it was lower than the values found by Nakaghi et al (2008) and Silva et al (2010) of 63.3% and 42.5%, respectively, in samples of domiciled domestic dogs from São Paulo and Mato Grosso. Souza et al (2010) conducted their study in domiciled domestic dogs in the city of Salvador, only 150 km away from the city of Ituberá; the two sites may have similar climatic conditions for the maintenance of the vector and similar risk factors associated with the infection, which might explain the similar seroprevalence values found at the two sites.…”

“…This bacterium mainly parasitizes the cells of the mononuclear phagocyte system and causes clinical and hematological abnormalities such as fever, anorexia, vomiting, diarrhea, petechial hemorrhages, anemia, and thrombocytopenia (MOREIRA et al, 2003). Canine monocytic ehrlichiosis is found in many tropical and subtropical countries and is found in all regions of Brazil (AGUIAR et al, 2007;ALBERNAZ et al, 2007;AZEVEDO et al, 2011;CARLOS et al, 2007;CARVALHO et al, 2008;KRAWCZAK et al, 2012;MUNDIM et al, 2008;SILVA et al, 2010). Some factors may predispose dogs to disease development, such as older age, the presence of and contact with other dogs, living in urban habitats, and exposure to R. sanguineus parasitism (AZEVEDO et al, 2011;FAIERSTEIN et al, 2008).…”

Quantitative analysis of bone marrow in pancytopenic dogs

“…The prevalence of pancytopenia found (5.4%) is higher than the reported elsewhere, a fact that is due to some infectious disease endemicity that cause it, as the CME (SILVA et al, 2010) and the CVL (ALMEIDA et al, 2012) that represented 35 (53.8%) cases. A study in the United States evaluated 4560 animals where 2.4% were pancyitopenics (WEISS et al, 1999).…”

“…Its importance increases as regards the diagnosis of endemic infectious diseases, as in the present study location: canine monocytic ehrlichiosis (CME) and canine visceral leishmaniasis (CVL) (SILVA et al, 2010;ALMEIDA et al, 2012). A variety of medullary responses to caused stress by various infections and parasitism have been related (DE TOMMASI et al, 2014).…”

Recombinant gp19 as a potential antigen for detecting anti-Ehrlichia canis antibodies in dog sera

“…Using an indirect immunofluorescence assay (IIFA), the seroprevalence rates in dogs were found to range from 4.8 to 42.5% in the Rio Grande do Sul (SAITO et al, 2008) and Mato Grosso (SILVA et al, 2010) states, respectively. Using a commercially available immunoenzymatic dot-enzyme-linked immunosorbent assay (ELISA) (SNAP  3DX, IDEXX Laboratories Inc., Portland, ME, USA) to test random populations, prevalence rates were found to range from 1.7 to 54.5% in dogs from the Rio Grande do Sul and Pernanbuco states, respectively (LABARTHE et al, 2003).…”