This study examined Reactive Orange 16 (RO16) adsorption onto biochar made from cannabis weed biomass (CWB) in aqueous solutions. The adsorption process is negatively impacted by increasing temperatures, which results in a shift toward system stability and energy loss. This indicates that the process is exothermic, as demonstrated by negative enthalpy change (ΔH) values. The negative values of ΔG at different temperatures indicate spontaneous adsorption, highlighting the practicality of using CWB to remove RO16 in practical applications. Monolayer adsorption on homogeneous sites within the CWB structure is indicated by the Langmuir isotherm model, which accurately represents the adsorption behavior. CWB's effectiveness in RO16 adsorption was demonstrated by the greatest adsorption capacity of 33.45 mg/g. Additionally, the starting dye concentration, adsorbent dose, and solution pH have a major impact on the adsorption effectiveness of CWB. The significance of accessible adsorption sites is highlighted because optimal dye removal happens at lower dye concentrations and greater adsorbent dosages. Another important factor is pH; lower pH values result in increased removal efficiency because of the advantageous electrostatic interactions that occur between the protonated surface of CWB and the anionic dye. For removing RO16 dye from aqueous solutions, CWB shows a great deal of promise as an economical, effective, and long-lasting adsorbent.