Sorption of humic acids by bacteria

Tikhonov, V. V.; Орлов, Д. С.; Lisovitskaya, O. V.; Zavgorodnyaya, Yu. A.; Byzov, B. A.; Демин, В. В.

doi:10.1134/s0026261713060106

Cited by 17 publications

(9 citation statements)

References 10 publications

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“…Humic substances are transformed by indigenous microbial communities [24,25], and may serve as a food source for soil microorganisms [26,27]. It has been shown that soil bacteria can absorb humic acids with Grampositive bacteria having the highest sorption potential [28]. Application of HS was shown to increase the total number of rhizosphere microorganisms, particularly the N-fixers, amylolytic, and cellulolytic microorganisms [29].…”

Section: Introductionmentioning

confidence: 99%

Impact of Bacillus subtilis on Tomato Plants Growth and Some Biochemical Characteristics under Combined Application with Humic Fertilizer

Pishchik

Vorobyov

Ostankova³

et al. 2018

IJPSS

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Section: Introductionmentioning

confidence: 99%

Impact of Bacillus subtilis on Tomato Plants Growth and Some Biochemical Characteristics under Combined Application with Humic Fertilizer

Pishchik

Vorobyov

Ostankova³

et al. 2018

IJPSS

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“…Our AFM images (Figure p,q) reveal a complete covering of the bacterial surface by HA molecules. The close associations between bacterial cells and humic acid have also been reported previously (Fein et al ., ; Tikhonov et al ., ; Du et al ., ). The blocking effect in our binary P. putida –HA complex results from not only the physical cover, but also from the surface complexation reactions between the functional groups on bacteria and humic acid, for example X‐H(L) + R‐COOH ↔ R‐COOH–H(L)‐X [X, R and H(L) represent humic acid, bacteria and any proton‐active organic functional group, respectively]; it results in a reduction of deprotonated adsorption sites.…”

Section: Resultsmentioning

confidence: 99%

Copper adsorption on composites of goethite, cells of Pseudomonas putida and humic acid

Lin

Chen

et al. 2017

European J Soil Science

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In the soil environment, iron oxides co‐occur commonly with different types of organic constituents to produce iron oxide–organic composites that play an important role in the biogeochemical cycling of trace metals. We investigated copper (Cu) adsorption on synthetic goethite, Pseudomonas putida (CCTCC M209319) bacterial cells, humic acid (HA) and their binary and ternary composites with batch adsorption experiments coupled with isothermal titration calorimetry (ITC). Morphological characterizations show that the three components can form closely combined and heterogeneous aggregates with one another. The kinetics of sorption of Cu to these composite materials conforms to the pseudo‐second‐order model, whereas the Cu sorptivities deviate from linear additivity. Specifically, Cu sorptivities on the binary goethite–P. putida and P. putida–HA and ternary goethite–P. putida–HA composites are less than expected assuming additivity, whereas the opposite is seen for the binary goethite–HA composite. There is considerable masking of adsorption sites in the ternary goethite–P. putida–HA system, but the binding sites on bacteria are not completely covered, as shown by the adsorption enthalpy and entropy (19.60 kJ mol−1 and 120.9 J mol−1 K−1, respectively) for the ternary composite. We conclude that the binding affinity of Cu for the binary goethite–humic acid composite is larger than that for the bacterial composites with goethite or humic acid, or both. This research indicates that bacteria, iron oxide and humic substances exhibit different behaviour in the sequestration of heavy metals when they form various binary and ternary complexes in natural environments. Highlights How does mineral–organic interaction affect the binding behaviour of trace elements? First investigation of Cu sorption to goethite–bacteria–humic acid composites. Goethite–HA shows enhanced Cu adsorption, whereas the opposite is true for bacterial complexes. Various bacteria–iron oxide–humic substance composites behave differently in metal sequestration.

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“…Additionally, it has been shown that the degree of sorption can depend on the cell wall structure in solution (Tikhonov et al . 2014), which was likely to be more negatively charged with increasing pH levels in liquid culture and could have led to the destruction E. coli O157 at pH 10·6.…”

Section: Discussionmentioning

confidence: 99%

Escherichia coli O157 survival in liquid culture and artificial soil microcosms with variable pH, humic acid and clay content

Baker

Schneider

2020

J Appl Microbiol

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Aims This research was performed to investigate the influence of clay and humic acid on Escherichia coli O157 survival in model soils. Additionally, the influence of pH and humic acid on E. coli O157 in liquid culture was investigated. Methods and Results Artificial soil microcosms were prepared with sand, kaolinite, bentonite and humic acid. Artificial soil microcosms pH was adjusted (6·0–7·0) with aluminium sulphate before E. coli O157 inoculation. After 56 days of incubation at 30°C, significant differences in E. coli O157 log CFU per gram were observed between 0 and 1000 ppm (P < 0·0001) and 0 and 5000 ppm (P < 0·0001) humic acid in 1·5% clay soils, but not in 7·5 or 15% clay soils. Significant differences (P < 0·05) in E. coli O157 log CFU per ml were observed in liquid culture influenced by humic acid concentrations after 8 h at 37°C. Conclusions The developed model soils support E. coli O157 populations over 28 days, and higher clay soils may aid in E. coli O157 survival. Significance and Impact of the Study These results provide insights into physicochemical properties of soil that may influence E. coli O157 in the environment and help explain E. coli O157 survival in various soils and geographical regions.

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Sorption of humic acids by bacteria

Cited by 17 publications

References 10 publications

Impact of Bacillus subtilis on Tomato Plants Growth and Some Biochemical Characteristics under Combined Application with Humic Fertilizer

Impact of Bacillus subtilis on Tomato Plants Growth and Some Biochemical Characteristics under Combined Application with Humic Fertilizer

Copper adsorption on composites of goethite, cells of Pseudomonas putida and humic acid

Escherichia coli O157 survival in liquid culture and artificial soil microcosms with variable pH, humic acid and clay content

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