Sorption of radioiodide in an acidic, nutrient-poor boreal bog: insights into the microbial impact

The microbial impact on the sorption behaviour of selenite in an acidic, nutrient-poor boreal bog

“…As only the innermost part of the sample was considered having remained oxygen free during sampling and transportation, the inner parts of the drill core sample were moved into sterile 50 mL centrifugal tubes in the nitrogen cabinet and stored in the nitrogen cabinet at 20 C. These samples were used for the experiments in anoxic conditions. Loss on ignition (LOI), humification degree, minerals in the gyttja and clay layer and pH of the samples were determined as described in Lusa et al (2015a). Microwave-assisted HNO 3 extraction was used to determine the overall iron (Fe) concentrations in the moss, peat, gyttja and clay samples.…”

“…The extract solutions were filtered through a 0.2-mm Supor membrane filter (Pall Corp., Port Washington, NY, USA), the samples were diluted to 1:33 and Fe concentrations were determined using inductively coupled plasma mass spectrometry (ICP-MS) (Agilent 7500ce, Agilent Technologies, Inc., Santa Clara, CA, USA). In addition bog water samples were separated from the surface and the depths of 0.5e1.0 m, 2.5e3.0 m and 5.5e6.0 m and major cations and anions were described in Lusa et al (2015a). A bog water model solution used in the determination of experimental K d values was prepared based on these determinations (Table 1) (Lusa et al, 2015a).…”

“…In addition bog water samples were separated from the surface and the depths of 0.5e1.0 m, 2.5e3.0 m and 5.5e6.0 m and major cations and anions were described in Lusa et al (2015a). A bog water model solution used in the determination of experimental K d values was prepared based on these determinations (Table 1) (Lusa et al, 2015a).…”

“…Bacterial suspensions were prepared by adding isolated bacterial colonies from the growth plates using a sterile loop into sterile water until the turbidity corresponded to a McFarland standard nro 6 to Table 1 Concentrations of major cations and anions in bog water and in the model bog water solution. The measured concentrations are the averages from three drill core samples (Lusa et al, 2015a). SeO 3 was added to obtain the 75 Se activity of 200 Bq/suspension.…”

“…The humification degree, organic matter content and pH of the peat samples and minerals in the gyttja and clay layers are reported in Lusa et al (2015a) (Table 2). In the <2 mm fraction of the gyttja and clay layers, illite, clinochlore and kaolinite were observed, and quartz, microcline, plagioclase, pyrite and Fe-hornblende in the >2 mm (Lusa et al, 2015a).…”