Sorption Properties of Biofilms

Flemming, Hans‐Curt; Leis, Andrew

doi:10.1002/0471263397.env285

Cited by 32 publications

(25 citation statements)

References 57 publications

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“…EPS may account for 50 to 90% of the total organic carbon of biofilms and is the primary matrix material of the biofilm (16). Knowing that biofilm formation in P. mirabilis was regulated by RsbA, EPS assays were performed to determine whether the production of EPS was also regulated by RsbA.…”

Section: Vol 72 2004 Fatty Acids Modulate Swarming and Virulence 6841mentioning

confidence: 99%

Modulation of Swarming and Virulence by Fatty Acids through the RsbA Protein inProteus mirabilis

Liaw

Lai

Wang³

2004

Infect Immun

101

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After sensing external signals, Proteus mirabilis undergoes a multicellular behavior called swarming which is coordinately regulated with the expression of virulence factors. Here we report that exogenously added fatty acids could act as signals to regulate swarming in P. mirabilis. Specifically, while oleic acid enhanced swarming, some saturated fatty acids, such as lauric acid, myristic acid, palmitic acid, and stearic acid, inhibited swarming. We also found that expression of hemolysin, which has been shown to be coordinately regulated with swarming, was also inhibited by the above saturated fatty acids. Previously we identified a gene, rsbA, which may encode a histidine-containing phosphotransmitter of the bacterial two-component signaling system and act as a repressor of swarming and virulence factor expression in P. mirabilis. We found that while myristic acid, lauric acid, and palmitic acid exerted their inhibitory effect on swarming and hemolysin expression through an RsbAdependent pathway, the inhibition by stearic acid was mediated through an RsbA-independent pathway. Biofilm formation and extracellular polysaccharide (EPS) production play an important role in P. mirabilis infection. We found that RsbA may act as a positive regulator of biofilm formation and EPS production. Myristic acid was found to slightly stimulate biofilm formation and EPS production, and this stimulation was mediated through an RsbA-dependent pathway. Together, these data suggest that fatty acids may act as environmental cues to regulate swarming and virulence in P. mirabilis and that RsbA may play an important role in this process.

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Section: Vol 72 2004 Fatty Acids Modulate Swarming and Virulence 6841mentioning

confidence: 99%

Modulation of Swarming and Virulence by Fatty Acids through the RsbA Protein inProteus mirabilis

Liaw

Lai

Wang³

2004

Infect Immun

101

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“…This matrix, referred to as the slime layer, glycocalyx, or extracellular polymeric substance (EPS) matrix, can comprise up to 90% of the biofilm biomass (8). In addition to its structural role, the EPS matrix provides biofilm cells with a protected microenvironment containing dissolved nutrients and secreted enzymes, as well as other biological molecules and abiotic substances originating from outside the biofilm.…”

mentioning

confidence: 99%

Genes Involved in the Synthesis and Degradation of Matrix Polysaccharide in Actinobacillus actinomycetemcomitans and Actinobacillus pleuropneumoniae Biofilms

et al. 2004

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Biofilms are composed of bacterial cells embedded in an extracellular polysaccharide matrix. A major component of the Escherichia coli biofilm matrix is PGA, a linear polymer of N-acetyl-D-glucosamine residues in ␤(1,6) linkage. PGA mediates intercellular adhesion and attachment of cells to abiotic surfaces. In this report, we present genetic and biochemical evidence that PGA is also a major matrix component of biofilms produced by the human periodontopathogen Actinobacillus actinomycetemcomitans and the porcine respiratory pathogen Actinobacillus pleuropneumoniae. We also show that PGA is a substrate for dispersin B, a biofilm-releasing glycosyl hydrolase produced by A. actinomycetemcomitans, and that an orthologous dispersin B enzyme is produced by A. pleuropneumoniae. We further show that A. actinomycetemcomitans PGA cross-reacts with antiserum raised against polysaccharide intercellular adhesin, a staphylococcal biofilm matrix polysaccharide that is genetically and structurally related to PGA. Our findings confirm that PGA functions as a biofilm matrix polysaccharide in phylogenetically diverse bacterial species and suggest that PGA may play a role in intercellular adhesion and cellular detachment and dispersal in A. actinomycetemcomitans and A. pleuropneumoniae biofilms.

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“…The biofilms may act as an ''insulating layer'' between the solution and the mineral surface or form ''micro-environments'' in which the local solution conditions are different than those in the bulk solution (Glazer et al 2002). Reactive functional groups, such as carboxyl, hydroxyl, amino and phosphoryl moieties, present on the bacterial surfaces and exopolysaccharide matrix, can provide a large array of binding sites for metals (Flemming & Leis 2002). For instance, autotrophic biofilms have been observed to sequester metals at 4-5 orders of magnitude above the concentration in the surrounding waters (Liehr et al 1994).…”

Section: Biofilms In Natural Environmentmentioning

confidence: 99%

Developments in Bioremediation of Soils and Sediments Polluted with Metals and Radionuclides – 1. Microbial Processes and Mechanisms Affecting Bioremediation of Metal Contamination and Influencing Metal Toxicity and Transport

Tabak

Lens

Hullebusch

et al. 2005

Rev Environ Sci Biotechnol

197

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Sorption Properties of Biofilms

Abstract: Sorption Sites in Biofilms Sorption of Water, Metals, Organic Substances, and Particles Remobilization

Cited by 32 publications

References 57 publications

Modulation of Swarming and Virulence by Fatty Acids through the RsbA Protein inProteus mirabilis

Modulation of Swarming and Virulence by Fatty Acids through the RsbA Protein inProteus mirabilis

Genes Involved in the Synthesis and Degradation of Matrix Polysaccharide in Actinobacillus actinomycetemcomitans and Actinobacillus pleuropneumoniae Biofilms

Developments in Bioremediation of Soils and Sediments Polluted with Metals and Radionuclides – 1. Microbial Processes and Mechanisms Affecting Bioremediation of Metal Contamination and Influencing Metal Toxicity and Transport

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