Sorting signals for PIN1 trafficking and localization

Marcote, María Jesús; Sáncho-Andrés, Gloria; Soriano-Ortega, Esther; Aniento, Fernando

doi:10.1080/15592324.2016.1212801

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…In parallel, we also investigated if PIN1 internalization happens through CME and further assessed if it is influenced by auxin (Supplemental Figure S6). To this end, we quantified the interaction between PIN1 and m2 adaptin, which is necessary for cargo recognition during CME (Marcote et al, 2016), using pull-down experiments with and without NAA treatment. We observed a positive m2-PIN1 interaction; however, there was no increase or decrease in the interaction in the presence of NAA.…”

Section: Constitutive Clathrin-mediated Pin2 Internalization Maintains Apical Pin2 Polaritymentioning

confidence: 99%

Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking

et al. 2021

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The phytohormone auxin and its directional transport through tissues are intensively studied. However, a mechanistic understanding of auxin-mediated feedback on endocytosis and polar distribution of PIN auxin transporters remains limited due to contradictory observations and interpretations. Here, we used state-of-the-art methods to reexamine the auxin effects on PIN endocytic trafficking. We used high auxin concentrations or longer treatments versus lower concentrations and shorter treatments of natural (IAA) and synthetic (NAA) auxins to distinguish between specific and nonspecific effects. Longer treatments of both auxins interfere with Brefeldin A-mediated intracellular PIN2 accumulation and also with general aggregation of endomembrane compartments. NAA treatment decreased the internalization of the endocytic tracer dye, FM4-64; however, NAA treatment also affected the number, distribution, and compartment identity of the early endosome/trans-Golgi network (EE/TGN), rendering the FM4-64 endocytic assays at high NAA concentrations unreliable. To circumvent these nonspecific effects of NAA and IAA affecting the endomembrane system, we opted for alternative approaches visualizing the endocytic events directly at the plasma membrane (PM). Using Total Internal Reflection Fluorescence (TIRF) microscopy, we saw no significant effects of IAA or NAA treatments on the incidence and dynamics of clathrin foci, implying that these treatments do not affect the overall endocytosis rate. However, both NAA and IAA at low concentrations rapidly and specifically promoted endocytosis of photo-converted PIN2 from the PM. These analyses identify a specific effect of NAA and IAA on PIN2 endocytosis, thus contributing to its polarity maintenance and furthermore illustrate that high auxin levels have nonspecific effects on trafficking and endomembrane compartments.

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Section: Constitutive Clathrin-mediated Pin2 Internalization Maintains Apical Pin2 Polaritymentioning

confidence: 99%

Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking

et al. 2021

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“…In N- and C-terminal domains located to cytoplasm of SaCLCa2, SaCLCc2, as well as of other SaCLC proteins consensus tyrosine-based motifs YXXΦ (Φ is a hydrophobic amino acid) and dileucine-based motifs DXXLL or [DE]XXXL[LI] were found ( Figure 1 , underlined). A key role of similar motifs in sorting and subcellular localization was shown for a number of membrane A. thaliana proteins [ 40 , 41 , 42 , 43 , 44 , 45 ].…”

Section: Resultsmentioning

confidence: 99%

“…These recruit components of the transport machinery, ensuring delivery of proteins to proper membranes [ 45 , 50 , 51 ]. Involvement of tyrosine- and dileucine-based motifs in sorting and control of subcellular localization of endocytosed plant proteins, such as auxin transporter PIN1 [ 40 ], vacuolar sorting receptor VSR4 [ 41 ], brassinosteroid receptor BRI1 [ 44 ], vacuolar transporters NRAMP3 и NRAMP4 [ 42 ], and SRK kinase [ 43 ], has been demonstrated. Analyses of amino acid sequences in S. altissima CLC proteins displayed presence of similar consensus motifs in N- and C-terminal domains located to cytoplasm ( Figure 1 , underlined).…”

Section: Discussionmentioning

confidence: 99%

Chloride Channel Family in the Euhalophyte Suaeda altissima (L.) Pall: Cloning of Novel Members SaCLCa2 and SaCLCc2, General Characterization of the Family

Nedelyaeva¹,

Попова²,

Khramov³

et al. 2023

IJMS

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CLC family genes, comprising anion channels and anion/H+ antiporters, are widely represented in nearly all prokaryotes and eukaryotes. CLC proteins carry out a plethora of functions at the cellular level. Here the coding sequences of the SaCLCa2 and SaCLCc2 genes, homologous to Arabidopsis thaliana CLCa and CLCc, were cloned from the euhalophyte Suaeda altissima (L.) Pall. Both the genes cloned belong to the CLC family as supported by the presence of the key conserved motifs and glutamates inherent for CLC proteins. SaCLCa2 and SaCLCc2 were heterologously expressed in Saccharomyces cerevisiae GEF1 disrupted strain, Δgef1, where GEF1 encodes the only CLC family protein, the Cl– transporter Gef1p, in undisrupted strains of yeast. The Δgef1 strain is characterized by inability to grow on YPD yeast medium containing Mn2+ ions. Expression of SaCLCa2 in Δgef1 cells growing on this medium did not rescue the growth defect phenotype of the mutant. However, a partial growth restoration occurred when the Δgef1 strain was transformed by SaCLCa2(C544T), the gene encoding protein in which proline, specific for nitrate, was replaced with serine, specific for chloride, in the selectivity filter. Unlike SaCLCa2, expression of SaCLCc2 in Δgef1 resulted in a partial growth restoration under these conditions. Analysis of SaCLCa2 and SaCLCc2 expression in the euhalophyte Suaeda altissima (L.) Pall by quantitative real-time PCR (qRT-PCR) under different growth conditions demonstrated stimulation of SaCLCa2 expression by nitrate and stimulation of SaCLCc2 expression by chloride. The results of yeast complementation assay, the presence of both the “gating” and “proton” glutamates in aa sequences of both the proteins, as well results of the gene expression in euhalophyte Suaeda altissima (L.) Pall suggest that SaCLCa2 and SaCLCc2 function as anion/H+ antiporters with nitrate and chloride specificities, respectively. The general bioinformatic overview of seven CLC genes cloned from euhalophyte Suaeda altissima is given, together with results on their expression in roots and leaves under different levels of salinity.

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Protein degrons and degradation: Exploring substrate recognition and pathway selection in plants

Isono,

Li,

Pulido

et al. 2024

The Plant Cell

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Proteome composition is dynamic and influenced by many internal and external cues, including developmental signals, light availability, or environmental stresses. Protein degradation, in synergy with protein biosynthesis, allows cells to respond to various stimuli and adapt by reshaping the proteome. Protein degradation mediates the final and irreversible disassembly of proteins, which is important for protein quality control and to eliminate misfolded or damaged proteins, as well as entire organelles. Consequently, it contributes to cell resilience by buffering against protein or organellar damage caused by stresses. Moreover, protein degradation plays important roles in cell signaling, as well as transcriptional and translational events. The intricate task of recognizing specific proteins for degradation is achieved by specialized systems that are tailored to the substrate’s physicochemical properties and subcellular localization. These systems recognize diverse substrate cues collectively referred to as “degrons”, which can assume a range of structural configurations. They are molecular surfaces recognized by E3 ligases of the ubiquitin-proteasome system, but can also be considered as general features recognized by other degradation systems, including autophagy or even organellar proteases. Here we provide an overview of the newest developments in the field, delving into the intricate processes of protein recognition and elucidating the pathways through which they are recruited for degradation.

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Sorting signals for PIN1 trafficking and localization

Cited by 5 publications

References 22 publications

Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking

Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking

Chloride Channel Family in the Euhalophyte Suaeda altissima (L.) Pall: Cloning of Novel Members SaCLCa2 and SaCLCc2, General Characterization of the Family

Protein degrons and degradation: Exploring substrate recognition and pathway selection in plants

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