Sources and propagation of errors in quantitative phase imaging techniques using optical interferometry

Bedrossian, Manuel; Nadeau, Jay; Serabyn, Eugene; Lindensmith, C. A.

doi:10.1117/12.2250069

Cited by 4 publications

(4 citation statements)

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“…For a sufficiently large timescale, it can be assumed that.ε = 0 The residual hologram is defined as the difference between Eqs. (2) and 3:…”

Section: Principle Of Operationmentioning

confidence: 99%

“…Digital off-axis holography is an imaging technique that is capable of capturing both the amplitude and phase of light using a single intensity image (hologram) [1]. It suffers from various types of noise, however, which include but are not limited to photon (shot and speckle) noise, and detector noise (dark noise, read noise, and quantization noise) [2]. Removal of the zero-order and twin-image terms is straightforward in off-axis holography [3,4], and much effort has been devoted to reducing residual noise in holographic reconstructions due to phase aberrations [5,6], astigmatism, spherical aberrations, and anamorphism [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

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Enhancing final image contrast in off-axis digital holography using residual fringes

et al. 2020

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We show that background fringe-pattern subtraction is a useful technique for removing static noise from off-axis holographic reconstructions and can enhance image contrast in volumetric reconstructions by an order of magnitude in the case for instruments with relatively stable fringes. We demonstrate the fundamental principle of this technique and introduce some practical considerations that must be made when implementing this scheme, such as quantifying fringe stability. This work also shows an experimental verification of the background fringe subtraction scheme using various biological samples.

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“…For a sufficiently large timescale, it can be assumed that.ε = 0 The residual hologram is defined as the difference between Eqs. (2) and 3:…”

Section: Principle Of Operationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancing final image contrast in off-axis digital holography using residual fringes

et al. 2020

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“…Because the typical values of n c for the cytoplasm and organelles are approximately 1.38, 11−13 which is very close to the n m of 1.33 for water, it is challenging for OADHM to visualize prokaryotic cells with much specificity, particularly when they are near the resolution limit of the instrument. 14 This challenge could be overcome with suitable contrast agents or reporter genes, which would make cells more visible or highlight subcellular features and processes.…”

Section: ■ Introductionmentioning

confidence: 99%

Genetically Encoded Phase Contrast Agents for Digital Holographic Microscopy

et al. 2020

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Quantitative phase imaging and digital holographic microscopy have shown great promise for visualizing the motion, structure and physiology of microorganisms and mammalian cells in three dimensions. However, these imaging techniques currently lack molecular contrast agents analogous to the fluorescent dyes and proteins that have revolutionized fluorescence microscopy. Here we introduce the first genetically encodable phase contrast agents based on gas vesicles, a unique class of air-filled protein nanostructures derived from buoyant microbes. The relatively low index of refraction of the air-filled core of gas vesicles results in optical phase advancement relative to aqueous media, making them a "positive" phase contrast agent easily distinguished from organelles, dyes, or microminerals. We demonstrate this capability by identifying and tracking the motion of gas vesicles and gas vesicle-expressing bacteria using digital holographic microscopy, and by imaging the uptake of engineered gas vesicles by mammalian cells. These results give phase imaging a biomolecular contrast agent, greatly expanding the capabilities of this powerful technology for three-dimensional biological imaging.

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“…Imagens da missão Cassini mostram jatos finos de partículas de gelo que emergem de seu polo sul. Esses jatos deram origem a uma extensa pluma que pode ser observada no espaço, indicando evidências da existência de moléculas orgânicas essenciais ao desenvolvimento da vida (McKay et al, 2008;Porco et al, 2006;Bedrossian et al, 2017). Caso seu oceano realmente exista, ele deverá ser salino e também aquecido por forças de maré (Zolotov, 2007).…”

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A Terra como um exoplaneta

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utilizada para futuras calibrações, pois responde linearmente com a variação da quantidade de material biológico.O resultado da pesquisa mostrou que existem bandas moleculares em comum entre a atmosfera e o material biológico, sendo atribuídas a potenciais marcadores moleculares que, possivelmente, poderão ser detectados de forma remota em futuras missões espaciais.Palavras-chave: bioassinaturas -espectroscopia no infravermelho -exoplanetas -DNA -extremófilos -atmosfera terrestre. AbstractIt is notorious that interest in detecting life beyond Earth has increased in the scientific world. The existence of possible biological markers the terrestrial atmosphere, the presence of living material or organic material decomposed on surfaces and at the sea increase the chances of success of the research. Life on Earth is everywhere and the planet is saturated with its manifestations.The objective of this thesis is the development of a methodology that o↵ers ideas for the detection of bioassinatures outside the Earth, particularly in exoplanets. In terms of molecular biology, the main signature of life is DNA (desoxyribonucleic acid), which organizes and hosts the genetic code all of the living things. Other molecules that denounce the presence of life are chlorophyll, carotenoids, DNA fragments, organic molecules, etc.In an attempt to find bioassinatures in the atmosphere of exoplanets, a requirement must be met: the identification of characteristic bands that allow the detection of a signal associated with a complex biomolecule, preferably in the infrared region, seen in the middle of the peaks of gases atmospheric conditions. Thus, a series of spectral analyzes were performed for DNA samples / cells of the extremophilic microorganism Halobacterium salinarum, to be compared to the infrared spectrum obtained directly from the Earth's atmosphere. The search for specific markers was performed in order to determine the peaks that allow the detection of these singular components when suspended in atmospheric gases.The results of the research show that the Earth's atmosphere is contaminated with complex molecules. There is a total of 37 common absorptions found in the spectrum of the Earth's atmosphere and in Cells or DNA spectra (for example 966, 936, 924, 886 and 866 cm 1 ). Among them, the peaks centered at approximately 1018, 996, 900 and 840

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Sources and propagation of errors in quantitative phase imaging techniques using optical interferometry

Abstract: Quantitative phase imaging (QPI) has many applications in a broad range of disciplines from astronomy to microbiology.

Cited by 4 publications

References 19 publications

Enhancing final image contrast in off-axis digital holography using residual fringes

Enhancing final image contrast in off-axis digital holography using residual fringes

Genetically Encoded Phase Contrast Agents for Digital Holographic Microscopy

A Terra como um exoplaneta

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