Sources of DNA Double-Strand Breaks and Models of Recombinational DNA Repair

Mehta, Anuja; Haber, James E.

doi:10.1101/cshperspect.a016428

Cited by 617 publications

(583 citation statements)

References 158 publications

(176 reference statements)

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“…These efforts have generated consensus models for HR, such as shown in Figures 1 and 3 (see Mehta and Haber 2014). Such positivistic projections of data and interpretations from diverse models and organisms into a single model have accelerated progress in the field but also bear the danger of ignoring informative species-specific differences.…”

Section: Resultsmentioning

confidence: 99%

“…Subpathway control within the HR process differentiates between BIR, synthesis-dependent strand-annealing (SDSA), and mechanisms involving double Holliday junction (dHJ), which determines crossover or noncrossover outcome (Fig. 1) (see Mehta and Haber 2014). Although dHJs are formed during somatic and meiotic HR, their processing differs, leading to crossover in meiosis and both outcomes in somatic cells (Schwacha and Kleckner 1995;Bzymek et al 2010;Bizard and Hickson 2014;Wyatt and West 2014;Yim et al 2014).…”

Section: Pathway Control Of Homologous Recombination Homologous Recommentioning

confidence: 99%

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Regulation of Recombination and Genomic Maintenance

Heyer

2015

Cold Spring Harb Perspect Biol

104

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Recombination is a central process to stably maintain and transmit a genome through somatic cell divisions and to new generations. Hence, recombination needs to be coordinated with other events occurring on the DNA template, such as DNA replication, transcription, and the specialized chromosomal functions at centromeres and telomeres. Moreover, regulation with respect to the cell-cycle stage is required as much as spatiotemporal coordination within the nuclear volume. These regulatory mechanisms impinge on the DNA substrate through modifications of the chromatin and directly on recombination proteins through a myriad of posttranslational modifications (PTMs) and additional mechanisms. Although recombination is primarily appreciated to maintain genomic stability, the process also contributes to gross chromosomal arrangements and copy-number changes. Hence, the recombination process itself requires quality control to ensure high fidelity and avoid genomic instability. Evidently, recombination and its regulatory processes have significant impact on human disease, specifically cancer and, possibly, neurodegenerative diseases.

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Section: Resultsmentioning

confidence: 99%

Section: Pathway Control Of Homologous Recombination Homologous Recommentioning

confidence: 99%

Regulation of Recombination and Genomic Maintenance

Heyer

2015

Cold Spring Harb Perspect Biol

104

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“…2). The pairing and annealing components of these pathways are conserved across the phylogenetic spectrum (see Mehta and Haber 2014).…”

Section: Roles Of Dna Pairing and Annealing In Hr Of Dna Dsbsmentioning

confidence: 99%

“…Break-induced replication (BIR) (see Mehta and Haber 2014) is a third type of HR transaction that is initiated by a pairing reaction be- tween the ssDNA tail of a resected end and the homologous duplex region of a sister chromatid (Llorente et al 2008;Anand et al 2013). There is no second-end capture by annealing in BIR, unlike in dHJ and SDSA.…”

Section: Break-induced Replicationmentioning

confidence: 99%

“…Heteroduplex DNA generated by meiotic COs also ensures proper segregation of homologous chromosomes, so that each gamete receives a complete but genetically distinct set of chromosomes (BascomSlack et al 1997;Gerton and Hawley 2005). In mitotic cells, heteroduplex DNA formation between sister chromatids is essential for homology-directed repair (HR) of DNA doublestrand breaks (DSBs), stalled replication forks, and other lesions (Maher et al 2011;Amunugama and Fishel 2012;Mehta and Haber 2014). Prokaryotic organisms also generate heteroduplex DNA to perform HR transactions, and to promote genetic exchanges, such as occur during bacterial conjugation (Cox 1999;Thomas and Nielsen 2005).…”

mentioning

confidence: 99%

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DNA-Pairing and Annealing Processes in Homologous Recombination and Homology-Directed Repair

Morrical

2015

Cold Spring Harb Perspect Biol

116

127

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The formation of heteroduplex DNA is a central step in the exchange of DNA sequences via homologous recombination, and in the accurate repair of broken chromosomes via homology-directed repair pathways. In cells, heteroduplex DNA largely arises through the activities of recombination proteins that promote DNA-pairing and annealing reactions. Classes of proteins involved in pairing and annealing include RecA-family DNA-pairing proteins, single-stranded DNA (ssDNA)-binding proteins, recombination mediator proteins, annealing proteins, and nucleases. This review explores the properties of these pairing and annealing proteins, and highlights their roles in complex recombination processes including the double Holliday junction (DhJ) formation, synthesis-dependent strand annealing, and singlestrand annealing pathways-DNA transactions that are critical both for genome stability in individual organisms and for the evolution of species.

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Control of Mus81 nuclease during the cell cycle

Pfander

Matos

2017

FEBS Letters

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Edited by Wilhelm JustDNA replication and homologous recombination involve the formation of branched DNA structures that physically link chromosomes. Such DNAbased connections, which arise during S-phase, are typically disengaged prior to entry into mitosis, in order to ensure proper chromosome segregation. Exceptions can, however, occur: replication stress, or elevated levels of DNA damage, may cause cells to enter mitosis with unfinished replication as well as carrying recombination intermediates, such as Holliday junctions. Hence, cells are equipped with pathways that recognize and process branched DNA structures, and evolved mechanisms to enhance their function when on the verge of undergoing cell division. One of these pathways utilizes the structure-selective endonuclease Mus81, which is thought to facilitate the resolution of replication and recombination intermediates. Mus81 function is known to be enhanced upon entry into M phase in budding yeast and human cells. Based on recent findings, we discuss here an updated model of Mus81 control during the cell cycle.

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Sources of DNA Double-Strand Breaks and Models of Recombinational DNA Repair

Cited by 617 publications

References 158 publications

Regulation of Recombination and Genomic Maintenance

Regulation of Recombination and Genomic Maintenance

DNA-Pairing and Annealing Processes in Homologous Recombination and Homology-Directed Repair

Control of Mus81 nuclease during the cell cycle

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