Sources of Errors in Molecular Testing

Chandler, Laura J.

doi:10.1016/b978-0-12-415783-5.00021-9

Cited by 2 publications

(3 citation statements)

References 53 publications

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“…122 New test methods also need to offer analysis at a reasonable cost for widespread clinical adoption and should ideally incorporate quality control to ensure the reliability of the test results. 123 Additionally, the exact clinical application may impose specific requirements and restrictions on assay designs that should be taken into account. 118 For example, assays to be used for screening of undiagnosed cancer need to possess a very low false-positive rate to minimize the impact on individuals with no disease.…”

Section: Acs Sensorsmentioning

confidence: 99%

“…Indeed, analytical methods that do not require highly trained personnel or the use of complex instrumentation will be more readily adaptable to the majority of clinical laboratories . New test methods also need to offer analysis at a reasonable cost for widespread clinical adoption and should ideally incorporate quality control to ensure the reliability of the test results . Additionally, the exact clinical application may impose specific requirements and restrictions on assay designs that should be taken into account .…”

Section: Opportunities and Challenges For Clinical Translation Of Snv...mentioning

confidence: 99%

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Targeted Detection of Single-Nucleotide Variations: Progress and Promise

Abi

Safavi

2019

ACS Sens.

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Advances in nucleic acid sequencing and genotyping technologies have facilitated the discovery of an increasing number of single-nucleotide variations (SNVs) associated with disease onset, progression, and response to therapy. The reliable detection of such disease-specific SNVs can ensure timely and effective therapeutic action, enabling precision medicine. This has driven extensive efforts in recent years to develop novel methods for the fast and cost-effective analysis of targeted SNVs. In this Review, we highlight the most recent and significant advances made toward the development of such methodologies.

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Section: Acs Sensorsmentioning

confidence: 99%

Section: Opportunities and Challenges For Clinical Translation Of Snv...mentioning

confidence: 99%

Targeted Detection of Single-Nucleotide Variations: Progress and Promise

Abi

Safavi

2019

ACS Sens.

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“…DNA microarrays or DNA chips are used for the detection of multiple genes, species, genotyping viruses, testing multiple single nucleotide polymorphisms (SNPs), or performing gene expression studies [8]. For this, oligonucleotides are attached to a solid surface.…”

Section: Introductionmentioning

confidence: 99%

In-silico Design of DNA Oligonucleotides: Challenges and Approaches

Hendling

Barišić

2019

Computational and Structural Biotechnology Journal

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DNA oligonucleotides are essential components of a high number of technologies in molecular biology. The key event of each oligonucleotide-based assay is the specific binding between oligonucleotides and their target DNA. However, single-stranded DNA molecules also tend to bind to unintended targets or themselves. The probability of such unspecific binding increases with the complexity of an assay. Therefore, accurate data management and design workflows are necessary to optimize the in-silico design of primers and probes. Important considerations concerning computational infrastructure and run time need to be made for both data management and the design process. Data retrieval, data updates, storage, filtering and analysis are the main parts of a sequence data management system. Each part needs to be well-implemented as the resulting sequences form the basis for the oligonucleotide design. Important key features, such as the oligonucleotide length, melting temperature, secondary structures and primer dimer formation, as well as the specificity, should be considered for the in-silico selection of oligonucleotides. The development of an efficient oligonucleotide design workflow demands the right balance between the precision of the applied computer models, the general expenditure of time, and computational workload. This paper gives an overview of important parameters during the design process, starting from the data retrieval, up to the design parameters for optimized oligonucleotide design.

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Sources of Errors in Molecular Testing

Cited by 2 publications

References 53 publications

Targeted Detection of Single-Nucleotide Variations: Progress and Promise

Targeted Detection of Single-Nucleotide Variations: Progress and Promise

In-silico Design of DNA Oligonucleotides: Challenges and Approaches

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