Sources of Resistance to Sunflower Diseasesin a Global Collection of Domesticated USDA Plant Introductions

Talukder, Zahirul I.; Hulke, Brent S.; Marek, Laura F.; Gulya, T. J.

doi:10.2135/cropsci2013.07.0506

Cited by 35 publications

(67 citation statements)

References 27 publications

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“…Broad‐sense heritability ( H 2 ), which estimates the proportion of phenotypic variance that is due to genetic factors, was moderately high (69%), lending further validation to our assumption that the observed variation for DI was mainly explained by the genetic makeup of the RILs. This result is consistent with our previous observations for this trait, where the genotype had much larger effect than the genotype × environment interaction (Talukder et al, 2014b). Despite significantly different environments and highly variable mean DI across environments, the Spearman's rank correlation coefficients for DI among environments were highly significant (Table 3), suggesting a general agreement of repeatability of the screening trials across different environments.…”

Section: Discussionsupporting

confidence: 93%

SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower using Genotyping‐by‐Sequencing

Talukder¹,

Seiler

Song

et al. 2016

The Plant Genome

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Basal stalk rot (BSR), caused by the ascomycete fungus Sclerotinia sclerotiorum (Lib.) de Bary, is a serious disease of sunflower (Helianthus annuus L.) in the cool and humid production areas of the world. Quantitative trait loci (QTL) for BSR resistance were identified in a sunflower recombinant inbred line (RIL) population derived from the cross HA 441 ´ RHA 439. A genotyping-bysequencing (GBS) approach was adapted to discover single nucleotide polymorphism (SNP) markers. A genetic linkage map was developed comprised of 1053 SNP markers on 17 linkage groups (LGs) spanning 1401.36 cM. The RILs were tested in five environments (locations and years) for resistance to BSR. Quantitative trait loci were identified in each environment separately and also with integrated data across environments. A total of six QTL were identified in all five environments: one of each on LGs 4, 9, 10, 11, 16, and 17. The most significant QTL, Qbsr-10.1 and Qbsr-17.1, were identified at multiple environments on LGs 10 and 17, explaining 31.6 and 20.2% of the observed phenotypic variance, respectively. The remaining four QTL, .1, were detected in only one environment on LGs 4, 9, 11, and 16, respectively. Each of these QTL explains between 6.4 and 10.5% of the observed phenotypic variation in the RIL population. Alleles conferring increased resistance were contributed by both parents. The potential of the Qbsr-10.1 and Qbsr-17.1 in marker-assisted selection (MAS) breeding are discussed. Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungus with a vast host range of >400 broadleaf species (Boland and Hall, 1994). The fungus causes three distinctly different diseases on sunflower: BSR or wilt, midstalk rot (MSR), and head rot. This fungal pathogen is characterized by its ability to produce long-term survival structures called sclerotia, which individually consist of masses of hyphae surrounded by a hard, black, protective rind. Depending on environmental conditions, sclerotia can germinate myceliogenically, and cause root infection, or carpogenically, producing apothecia then ascospores and infect aboveground parts of host plants (Gulya et al., 1997;Bolton et al., 2006). Unlike other hosts, BSR symptoms start from a root infection resulting from myceliogenic germination of sclerotia. Midstalk rot commonly begins as a leaf infection, while head rot infection begins on capitula. Both MSR and head rot symptoms are incited by airborne ascospores Univ., Fargo, ND 58102, USA. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer.

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Section: Discussionsupporting

confidence: 93%

SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower using Genotyping‐by‐Sequencing

Talukder¹,

Seiler

Song

et al. 2016

The Plant Genome

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“…Currently, there are no cultivated sunflower inbred lines or commercial hybrids that possess an acceptable level of resistance to Sclerotinia (Hahn, 2002; Gulya, 2005; Talukder et al, 2014, 2016). In an effort to manage sunflower Sclerotinia disease, numerous wild Helianthus species were screened for their reaction to Sclerotinia head rot and stalk rot.…”

Section: Discussionmentioning

confidence: 99%

“…The latter two diseases cause serious losses of yield and seed quality during epidemic years under appropriate environmental conditions. Field evaluation of germplasm for resistance to Sclerotinia BSR and HR revealed that there was no correlation between the two, suggesting a different inheritance of resistance to Sclerotinia in sunflower (Gulya et al, 1989; Talukder et al, 2014). …”

Section: Introductionmentioning

confidence: 99%

“…No complete resistance has been identified in cultivated sunflower or in its wild relatives. However, quantitative genetic variations among some varieties have been described (Tourvieille et al, 1996; Degener et al, 1998, 1999; Gulya et al, 2010; Talukder et al, 2014). Previous efforts to characterize BSR resistance indicated that the trait is quantitatively inherited for which the majority of the genetic variation is due to additive gene effects (Mestries et al, 1998; Van Becelaere and Miller, 2004; Talukder et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Genotyping-by-Sequencing Uncovers the Introgression Alien Segments Associated with Sclerotinia Basal Stalk Rot Resistance from Wild Species—I. Helianthus argophyllus and H. petiolaris

Long

Talukder

et al. 2016

Front. Genet.

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Basal stalk rot (BSR), caused by Sclerotinia sclerotiorum, is a devastating disease in sunflower worldwide. The progress of breeding for Sclerotinia BSR resistance has been hampered due to the lack of effective sources of resistance for cultivated sunflower. Our objective was to transfer BSR resistance from wild annual Helianthus species into cultivated sunflower and identify the introgressed alien segments associated with BSR resistance using a genotyping-by-sequencing (GBS) approach. The initial crosses were made between the nuclear male sterile HA 89 with the BSR resistant plants selected from wild Helianthus argophyllus and H. petiolaris populations in 2009. The selected resistant F1 plants were backcrossed to HA 458 and HA 89, respectively. Early generation evaluations of BSR resistance were conducted in the greenhouse, while the BC2F3 and subsequent generations were evaluated in the inoculated field nurseries. Eight introgression lines; six from H. argophyllus (H.arg 1 to H.arg 6), and two from H. petiolaris (H.pet 1 and H.pet 2), were selected. These lines consistently showed high levels of BSR resistance across seven environments from 2012 to 2015 in North Dakota and Minnesota, USA. The mean BSR disease incidence (DI) for H.arg 1 to H.arg 6, H.pet 1, and H.pet 2 was 3.0, 3.2, 0.8, 7.2, 7.7, 1.9, 2.5, and 4.4%, compared to a mean DI of 36.1% for Cargill 270 (susceptible hybrid), 31.0% for HA 89 (recurrent parent), 19.5% for HA 441 (resistant inbred), and 11.6% for Croplan 305 (resistant hybrid). Genotyping of the highly BSR resistant introgression lines using GBS revealed the presence of the H. argophyllus segments in linkage groups (LGs) 3, 8, 9, 10, and 11 of the sunflower genome, and the H. petiolaris segments only in LG8. The shared polymorphic SNP loci in the introgression lines were detected in LGs 8, 9, 10, and 11, indicating the common introgression regions potentially associated with BSR resistance. Additionally, a downy mildew resistance gene, Pl17, derived from one of the parents, HA 458, was integrated into five introgression lines. Germplasms combining resistance to Sclerotinia BSR and downy mildew represent a valuable genetic source for sunflower breeding to combat these two destructive diseases.

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“…Imidazolinone herbicide tolerance testing was conducted using the field method described by Hulke and Gulya (2015), in which 20 plants were backpack sprayed with a 2× labeled rate of imazamox (Beyond herbicide, BASF). Sclerotinia head rot (HR) and Sclerotinia stalk rot (SR) evaluation of testcrosses was conducted at Carrington and Casselton, ND, respectively, in 2004 and 2005 using the method described by Hulke and Gulya (2015) and Talukder et al (2014). For HR evaluation, field‐grown plants were inoculated at bloom with lab‐produced ascospores of S. sclerotiorum isolate ‘NEB‐274’ at a concentration of 5 × 10 3 spores mL −1 in distilled water.…”

Section: Methodsmentioning

confidence: 99%

Registration of Oilseed Sunflower Germplasms RHA 461, RHA 462, RHA 463, HA 465, HA 466, HA 467, and RHA 468 with Diversity in Sclerotinia Resistance, Yield, and Other Traits

Hulke

Ma²,

et al. 2017

J of Plant Registrations

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Sclerotinia head and stalk rots are among the most devastating diseases in sunflower (Helianthus annuus L.). Breeding for resistance to these diseases while maintaining or improving yield and quality is complicated by the quantitative inheritance of the resistance, for which there are few genes of major effect. The objective of this work was to provide new diversity to sunflower breeding organizations for yield and Sclerotinia resistance, together with other useful agronomic and end user traits. Four restorer germplasms, RHA 461 (Reg. No. GP‐337, PI 655012), RHA 462 (Reg. No. GP‐338; PI 655013), RHA 463 (Reg. No. GP‐339; PI 655014), and RHA 468 (Reg. No. GP‐341; PI 667184), and three maintainer germplasms, HA 465 (Reg. No. GP‐342; PI 670488), HA 466 (Reg. No. GP‐340; PI 667183), and HA 467 (Reg. No. GP‐343; PI 670489) were developed by the USDA‐ARS and the North Dakota Agricultural Experiment Station, Fargo, ND. Testcrosses with these germplasms had yield similar to high‐yielding commercial checks and Sclerotinia resistance similar to the highly resistant check ‘Northrup King 277’. Some of the lines also have high oleic acid in the seed oil, downy mildew resistance, and tolerance to imidazolinone herbicides. The lines are available for sunflower breeders to integrate into their breeding programs for additional diversity.

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Sources of Resistance to Sunflower Diseasesin a Global Collection of Domesticated USDA Plant Introductions

Cited by 35 publications

References 27 publications

SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower using Genotyping‐by‐Sequencing

SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower using Genotyping‐by‐Sequencing

Genotyping-by-Sequencing Uncovers the Introgression Alien Segments Associated with Sclerotinia Basal Stalk Rot Resistance from Wild Species—I. Helianthus argophyllus and H. petiolaris

Registration of Oilseed Sunflower Germplasms RHA 461, RHA 462, RHA 463, HA 465, HA 466, HA 467, and RHA 468 with Diversity in Sclerotinia Resistance, Yield, and Other Traits

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