Sources of Stem Rust Resistance in Wheat-Alien Introgression Lines

Rahmatov, Mahbubjon; Rouse, Matthew N.; Steffenson, Brian J.; Andersson, Staffan; Wanyera, R.; Pretorius, Z. A.; Houben, Andreas; Nazari, K.; Bhavani, Sridhar; Johansson, Eva

doi:10.1094/pdis-12-15-1448-re

Cited by 39 publications

(33 citation statements)

References 72 publications

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“…This necessitates a constant search for new sources of resistance and development of varieties with durable resistance. Global efforts are currently underway to explore wheat germplasm collections maintained in gene banks for new sources of resistance and other agriculturally useful traits (Gurung et al., 2014; Kertho et al., 2015; Maccaferri et al., 2015a,b; Muleta et al., 2017a; Rahmatov et al., 2016; Sehgal et al., 2015). The ability to efficiently unlock the genetic diversity of wheat germplasm collections, and their utilization in wheat breeding has become more feasible with the recent improvements in genotyping technology and statistical models for quantitative trait analysis (Cavanagh et al., 2013; Wang et al., 2014).…”

Section: Discussionmentioning

confidence: 99%

Genome‐wide mapping of resistance to stripe rust caused by Puccinia striiformis f. sp. tritici in hexaploid winter wheat

2020

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Stripe rust, caused by the fungus Puccinia striiformis f. sp. tritici (Pst), is a widespread disease and major limit to wheat (Triticum aestivum L.) production worldwide. The USDA‐ARS National Small Grains Germplasm Collection has proven to be a rich source of genetic diversity to improve stripe rust resistance in wheat breeding programs. The objective of the present study was to investigate the genetic diversity and sources of resistance to stripe rust in 441 accessions from the collection representing globally sourced winter wheat germplasm, as a complement to a previous study. A genome‐wide association study was conducted to identify loci conferring resistance to Pst based on phenotypic data from four field experiments and greenhouse seedling resistance screening against three races of the pathogen. A total of 5831 single nucleotide polymorphism markers were used to investigate population structure, linkage disequilibrium, and marker–trait associations. Our results showed 12 and 7 genomic regions significantly associated with Pst resistance based on field adult‐plant and greenhouse seedling responses, respectively, at a false discovery rate adjusted P value of <0.1. Four of the significantly associated genomic regions were mapped far from previously identified Pst resistance genes and quantitative trait loci, indicating that they represent potentially new Pst resistance loci. The present study provides additional insight into the usefulness of wheat germplasm collections as an important resistance source that can be used to incorporate diverse resistance genes into adapted wheat cultivars. Further work should aim to validate the identified genomic regions and the associated molecular markers to enhance their utility in marker‐assisted breeding.

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Section: Discussionmentioning

confidence: 99%

Genome‐wide mapping of resistance to stripe rust caused by Puccinia striiformis f. sp. tritici in hexaploid winter wheat

2020

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“…Previous studies on these wheat-alien introgression lines have revealed the presence of new sources of resistance against stem rust and the Russian wheat aphid (Diuraphis noxia) (Andersson et al, 2015;Rahmatov et al, 2016). Wheat-alien introgression lines are recognized as a valuable source of resistance for future wheat cultivars.…”

Section: Discussionmentioning

confidence: 99%

Seedling and Adult Plant Stripe Rust Resistance in Diverse Wheat–Alien Introgression Lines

Rahmatov

Hovmøller

Nazari³

et al. 2017

Crop Science

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Stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici Eriks., is an important foliar disease of wheat (Triticum aestivum L.) worldwide. The use of genetic diversity, particularly from wild relatives, has made a significant contribution to improving wheat productivity. To identify new sources of seedling and adult plant resistance, we evaluated 263 wheat–alien introgression derivatives from Secale cereale L., Leymus mollis (Trin.) Plig., Leymus racemosus (Lam.) Tzevlev, and Thinopyrum junceiforme (Á.Löve & D.Löve) Á. Löve. Three resistance genes, Yr1+, Yr2+, and Yr9+ were postulated to be present in 7, 19, and 12% of wheat–alien introgression lines, respectively. In addition, the results suggested the gene combinations of Yr1–Yr2 (1%), Yr1–Yr32 (2%), Yr2–Yr9 (5.4%), Yr1–Yr2–Yr32 (11%), and the additional unknown resistance gene(s) being present in these materials in the respective percentages. The molecular markers Xwmc364 for Yr2 (36%), Xscm9 and Xiag95 for Yr9 (17%), and Xwmc198 for Yr32 (13%) were used to validate the presence of these Yr genes. Ninety‐eight (37%) of the lines possess unknown resistance genes that could not be determined with the isolates used in this study and six (2.3%) of the lines showed seedling resistance to all isolates. The lines with the putative new resistance gene(s) will be crossed to the adapted genotypes to develop a mapping population for determining the underlying genetic basis of resistance. If these resistance genes are validated as being new, they will enhance the genetic diversity for stripe rust resistance in wheat.

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“…Infection types were scored 14 days after inoculation using a 0-4 scale (Stakman et al 1962;Long and Kolmer 1989). Seedling resistance to Pgt race TKTTF (bulk collection from Turkey) was carried out at the Regional Cereal Rust Research Center (RCRRC), located at the Aegean Agricultural Research Institute, International Center for Agricultural Research in the Dry Areas (ICARDA) in Izmir, Turkey (Rahmatov et al 2016). The methods used for this test were similar to those used for the other races, the exception being that fresh urediniospores collected from plants in the field were used instead of frozen urediniospores.…”

Section: Seedling Rust Resistance Assaysmentioning

confidence: 99%

Characterization of stem, stripe and leaf rust resistance in Tajik bread wheat accessions

et al. 2019

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Stem rust [causal organism: Puccinia graminis f. sp. tritici (Pgt)], stripe rust [Puccinia striiformis f. sp. tritici (Pst)], and leaf rust [Puccinia triticina (Pt)] are important fungal diseases of wheat in Central Asia and worldwide. Therefore, identification of seedling and adult plant resistance (APR) genes is of major importance for the national wheat breeding program in many countries. The objectives of this study were to identify genes that confer seedling and APR resistances in widely grown wheat cultivars, landraces and advanced lines from Tajikistan. A total of 41 wheat accessions were inoculated with eleven races of Pgt, twelve races of Pst and nine races of Pt for postulation of Sr (stem rust), Yr (yellow or stripe rust), and Lr Lr (leaf rust) resistance genes at the seedling stage. In addition, all of the accessions were tested in field trials for the response to stem rust and stripe rust. Genes for seedling stem rust resistance (i.e. Sr5, Sr6, Sr11, Sr31, and Sr38), stripe rust resistance (Yr9, Yr17, and Y27), and leaf rust resistance (Lr16 and Lr26) were postulated in the Tajik wheat. The presence of the pleiotropic APR genes Sr2/Yr30/ Lr27 (associated with pseudo-black chaff phenotype) and Lr34/Yr18/Sr57 (associated with leaf tip necrosis phenotype), and also Lr37 were assessed in the field and confirmed with linked molecular markers. In most of the wheat accessions, resistance genes could not be postulated because their infection types did not match the avirulence or virulence profile of the Pgt, Pst and Pt races tested. Six, seven, and nine accessions were identified that likely possess new genes for resistance to stem rust, stripe rust, and leaf rust, respectively, which have not been described previously. The research demonstrates the presence of effective seedling resistance and APR genes in widely grown wheat accessions that could facilitate further rust

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Sources of Stem Rust Resistance in Wheat-Alien Introgression Lines

Cited by 39 publications

References 72 publications

Genome‐wide mapping of resistance to stripe rust caused by Puccinia striiformis f. sp. tritici in hexaploid winter wheat

Genome‐wide mapping of resistance to stripe rust caused by Puccinia striiformis f. sp. tritici in hexaploid winter wheat

Seedling and Adult Plant Stripe Rust Resistance in Diverse Wheat–Alien Introgression Lines

Characterization of stem, stripe and leaf rust resistance in Tajik bread wheat accessions

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