This article deals with the factors influencing the degree of trust in political institutions at three levels of government in the Czech Republic (national, regional and local) in two dissimilar regions in the time of economic crisis in 2009, the year when citizens of the Czech Republic experienced the negative impacts of growing unemployment and a substantial decline in real GDP. Two competitive theories - the cultural and the performance explanation - were used as a theoretical framework. The results show that there are significant differences among particular levels of government as well as regions. The influence of both institutional performance, including factors related to economic crisis, and cultural background were found. The influence of contextual factors was also confirmed.