The critically endangered Florida grasshopper sparrow (Ammodramus savannarum floridanus) is dependent on dry prairies managed with prescribed fires that are applied every 1–3 years. Timing of prescribed fires shifted from predominantly the dormant season (Jan–Mar) to the transition (Apr–Jun) and growing (Jul–Aug) seasons starting in the early 2000s to better mimic natural fire regimes. Populations have continued to decline, however, and the presumption that natural fire regimes are beneficial to the small extant Florida grasshopper sparrow populations needs to be evaluated. We monitored 167 Florida grasshopper sparrow nests across 6 fire treatments at Three Lakes Wildlife Management Area, Osceola County, Florida, USA, 2013–2016, and investigated the role of fire season and interval on clutch initiation date, nest site selection, nest survival, productivity, and nestling mass. Clutch initiation was delayed by ≥33 days in prairies burned during the current transition season and we found no nests in prairies burned during the current growing season. Transition season burns also overlapped with the early breeding season, which could result in the destruction of nests. Clutches were larger at the beginning of the breeding season and nestling mass was highest in the middle of the breeding season, indicating that early and mid‐season nests may be more valuable to annual fecundity than late‐season nests. Florida grasshopper sparrows nested in prairies burned in the current dormant season or previous growing season more than expected based on their availability and as expected or less than expected in prairies burned in the current transition season, previous transition season, previous dormant season, or >2 years ago. However, we found no effect of fire treatment on daily nest survival, clutch size, number of fledglings per successful nest, or nestling mass. We recommend dormant season burning in prairies occupied by Florida grasshopper sparrows to increase reproductive potential given the subspecies’ small population and the importance of each nest attempt to population growth. We recommend transition or growing season burns in unoccupied adjacent prairies to maintain high‐quality dry prairie for nesting in future years. Prescribed fire management goals for Florida grasshopper sparrow populations should be guided by demographic responses rather than natural fire regimes. © 2019 The Wildlife Society.