Southern Brazilian autumnal propolis shows anti-angiogenic activity: An in vitro and in vivo study

Meneghelli, Cristiane; Joaquim, Lisiê Silva Dalsasso; Félix, Giovanni Loos Queiroz; Somensi, Amélia; Tomazzoli, Maíra Maciel; Silva, Diego Adílio da; Berti, Fernanda Vieira; Veleirinho, Maria Beatriz; Recouvreux, Derce de Oliveira Souza; Zeri, Ana Carolina de Mattos; Dias, Paulo Fernando; Maraschin, Marcelo

doi:10.1016/j.mvr.2013.03.003

Cited by 30 publications

(24 citation statements)

References 54 publications

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“…Quercetin is a ubiquitous constituent of propolis, a hive sealant derived from plant resins that is typically rich in flavonols and other phenolics. In Brazil, Nicodemo et al [20] found that honey bee longevity is 6.6% greater in hives with more propolis present; propolis typical of this region has been shown to be rich in quercetin, along with phenolic acids [21]. …”

Section: Introductionmentioning

confidence: 99%

Impacts of Dietary Phytochemicals in the Presence and Absence of Pesticides on Longevity of Honey Bees (Apis mellifera)

Liao

Wen

Berenbaum

2017

Insects

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Because certain flavonols and phenolic acids are found in pollen and nectar of most angiosperms, they are routinely ingested by Apis mellifera, the western honey bee. The flavonol quercetin and the phenolic acid p-coumaric acid are known to upregulate detoxification enzymes in adult bees; their presence or absence in the diet may thus affect the toxicity of ingested pesticides. We conducted a series of longevity assays with one-day-old adult workers to test if dietary phytochemicals enhance longevity and pesticide tolerance. One-day-old bees were maintained on sugar syrup with or without casein (a phytochemical-free protein source) in the presence or absence of quercetin and p-coumaric acid as well as in the presence or absence of two pyrethroid insecticides, bifenthrin and β-cyfluthrin. Dietary quercetin (hazard ratio, HR = 0.82), p-coumaric acid (HR = 0.91) and casein (HR = 0.74) were associated with extended lifespan and the two pyrethroid insecticides, 4 ppm bifenthrin (HR = 9.17) and 0.5 ppm β-cyfluthrin (HR = 1.34), reduced lifespan. Dietary quercetin enhanced tolerance of both pyrethroids; p-coumaric acid had a similar effect trend, although of reduced magnitude. Casein in the diet appears to eliminate the life-prolonging effect of p-coumaric acid in the absence of quercetin. Collectively, these assays demonstrate that dietary phytochemicals influence honey bee longevity and pesticide stress; substituting sugar syrups for honey or yeast/soy flour patties may thus have hitherto unrecognized impacts on adult bee health.

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Section: Introductionmentioning

confidence: 99%

Impacts of Dietary Phytochemicals in the Presence and Absence of Pesticides on Longevity of Honey Bees (Apis mellifera)

Liao

Wen

Berenbaum

2017

Insects

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“…It has been shown that A. angustifolia species contain phenolic acids (Table 2), being gallic acid ( Figure 5E) the most common one. This compound was already detected in the seeds [9] and in the propolis [39], whereas benzoic acid ( Figure 5F) was identified in the bark [12], and also in the propolis [39]. Exudate from leaves presents p-coumaric acid [43], which was also found in the propolis [10] along with dihydrocinnamic, ferulic and caffeic acids [38].…”

Section: Phenolic Acids and Their Derivativesmentioning

confidence: 82%

“…Exudate from leaves presents p-coumaric acid [43], which was also found in the propolis [10] along with dihydrocinnamic, ferulic and caffeic acids [38]. Some other phenolic acids, including protocatechuic acid (9.1 μg/mL) and ester derivatives of chlorogenic acid (7.9 μg/mL), were quantified in A. angustifolia propolis along with minor amounts (<1.8 μg/ mL) of p-coumaric, syringic, vanillic, caffeic and ferulic acids [39].…”

Section: Phenolic Acids and Their Derivativesmentioning

confidence: 89%

“…Cordenunsi et al [6] described that, in the seed, phenolic compounds can migrate Propolis Ethanol TLC/CG/CG-MS 5,6,7-trihydroxy-3,4'-dimethoxyflavone P , kaempferid P , aromadendrine-4'-methyl ether P , E and Z 2,2-dymethyl-6-carboxyethenyl-8-prenyl-2H-benzopyranes P , dihydrocinnamic acid P , p-coumaric acid P , ferulic acid P , caffeic acid P and di-and triterpenes T [38] Propolis Ethanol/water HPLC/UV spectrophotometry/NMR Quercetin P , rutin P , gallic acid P , protocatechuic acid P , chlorogenic acid P and its esther derivates, p-coumaric acid P , syringic acid P , vanillic acid P , caffeic acid P and ferulic acids P [39] Resin (knot) Chloroform/methanol TLC/ 1 H and 13 C NMR Hinokiresinol P , cryptoresinol P , secoisolariciresinol P , isolariciresinol P , pinoresinol monomethyl ether P , 2,3-bis-(p-hydroxyphenyl)-2-cyclopentene-1-one P and 4,4'-dihydroxychalcone P [11] Resin (wood) Dichloromethane/ methanol GC/MS Lignans P , 4-hydroxybenzaldehyde P , hydroquinone P , p-coumaric acid P , ferruginol diterpene T [10] Seedling stages Ethanol TLC/MS/NMR E and Z octadecyl ferulate P , biflavones P , benzaldehydes P and its derivates, vanillin P , pinoresinol P , eudesmin P , lariciresinol P , cabreuvine P , irisolidone P and diterpenes T Analysis Methods: gas chromatography (GC); liquid chromatography (LC); thin layer chromatography (TLC); high performance liquid chromatography (HPLC); high performance thin layer chromatography (HPTLC); high resolution mass spectrometry (HRMS); multiple reaction monitoring mode (MRM); mass spectrometry (MS); nuclear magnetic resonance (NMR); not described (ND).…”

Section: Polyphenolsmentioning

confidence: 99%

“…Moreover, changes in cell morphology were observed, including lysis and disorganization of the cellular monolayer. In another study, Meneghelli et al [39] reported the effects of A. angustifolia propolis on viability, proliferation, cell migration, capillary tube formation, and angiogenesis using in vitro and in vivo models. Hydroalcoholic extract of propolis was able to decrease viability in 24 (IC 50 297 µg/mL) and 72 (IC 50 130 µg/mL) hours, proliferation (at doses of 130 to 180 µg/mL for 72 h) and tubulogenesis (at doses of 150 to 200 µg/mL for 24 h) on human endothelial cells.…”

Section: Antiproliferative and Cytotoxic Activitiesmentioning

confidence: 99%

See 2 more Smart Citations

Chemical Constituents and Biological Activities of Araucaria angustifolia (Bertol.) O. Kuntze: A Review

Branco¹,

Rodrigues²,

Lima³

et al. 2016

J Org Inorg Chem

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Araucaria angustifolia is a tree that belongs to Araucariaceae family and it is mainly found in Southern Brazil. This plant has a notable therapeutical history in folk medicine holding great socioeconomic and environmental importance. Until now, some studies were conducted to assess its chemical composition, biological and pharmacological properties. The studies have shown that the bark, knot, needles (leaves), seeds and bracts (sterile seeds) contain high concentrations of active compounds and exhibit different biological effects. In the folk medicine the different parts of this plant are used to treat various types of illnesses, such as shingles, respiratory tract infections, sexually transmitted diseases and some types of wounds. Bearing this in mind, this review focuses on all currently chemical and biological effects already reported for A. angustifolia and provide a novel perspective and useful information for future research.

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Mollicute Anti‐Adhesive and Growth Inhibition Properties of the Methanolic Extract of Propolis from the Brazilian Native Bee Melipona quadrifasciata

Galgowski

Frare

Rau

et al. 2020

Chemistry & Biodiversity

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Hydroalcoholic propolis extracts from the bee species Melipona quadrifasciata have been shown to possess antimicrobial activity against different mollicute strains, but a methanolic extract (ME) could contain an increased diversity of nonpolar bioactive components with a potentially higher antimicrobial activity. The ME obtained by maceration of the propolis sample was fractionated with solvents of different polarities and then, purified by silica gel column chromatography through biomonitoring of its antimicrobial activity against mollicute strains. Analysis by gas chromatography-mass spectrometry (GC/MS) enabled the identification of compounds using the NIST library. Minimum inhibitory concentrations (MICs) of the samples were determined by broth microdilution. Anti-adhesive assays were performed with Mycoplasma pneumoniae cells. The hexane (MIC = 62.5 mg/L) and dichloromethane (MIC = 125 mg/L) fractions presented the most promising results against M. pneumoniae. They were fractionated into 74 subfractions, and even the best ones did not show better results (MIC > 250 mg/L) than their original fractions, likely due to the loss of terpene compounds that seem to act in synergy. The dichloromethane subfraction FD4 was highlighted in the anti-adhesive assay with an inhibitory activity of 21.6 %. A synergistic effect of the nonpolar compounds in M. quadrifasciata propolis may be responsible for its antibacterial activity, but several purified components can improve its anti-adhesive properties.

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Southern Brazilian autumnal propolis shows anti-angiogenic activity: An in vitro and in vivo study

Cited by 30 publications

References 54 publications

Impacts of Dietary Phytochemicals in the Presence and Absence of Pesticides on Longevity of Honey Bees (Apis mellifera)

Impacts of Dietary Phytochemicals in the Presence and Absence of Pesticides on Longevity of Honey Bees (Apis mellifera)

Chemical Constituents and Biological Activities of Araucaria angustifolia (Bertol.) O. Kuntze: A Review

Mollicute Anti‐Adhesive and Growth Inhibition Properties of the Methanolic Extract of Propolis from the Brazilian Native Bee Melipona quadrifasciata

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