Sox1 directly regulates the gamma -crystallin genes and is essential for lens development in mice

Nishiguchi, Seiji; Wood, Heather; Kondoh, Hisato; Lovell-Badge, Robin; Episkopou, Vasso

doi:10.1101/gad.12.6.776

Cited by 267 publications

(217 citation statements)

References 29 publications

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“…Sox1, a Group B1 SRY-like HMG box transcription factor, is required for fiber cell development in mice (Nishiguchi et al, 1998). Mice lacking Sox1 have small, opaque lenses with a persistent hole in the central cavity where fiber cells fail to fully differentiate (Nishiguchi et al, 1998).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Mice lacking Sox1 have small, opaque lenses with a persistent hole in the central cavity where fiber cells fail to fully differentiate (Nishiguchi et al, 1998). Sox1 null lenses lose expression of γ-crystallin at E12.5 coincident with the downregulation of the related gene, Sox2 (Nishiguchi et al, 1998). Although loss of γ-crystallin has been linked to cataract formation (reviewed in Graw, 2004), it is reasonable to hypothesize that other genes downstream of Sox1 also contribute to the fiber cell differentiation defect.…”

Section: Resultsmentioning

confidence: 99%

“…Sox1 βgeo , Sox1 M1 , and Pax6 1-NeuSey/+ (Pax6 Sey/+ ) mice were maintained on a C3H/HeN background (Nishiguchi et al, 1998;Ekonomou et al, 2005). Sox1 βgeo and Sox1 M1 heterozygotes (Sox1 +/− ) were crossed to generate Sox1 −/− embryos (Ekonomou et al, 2005).…”

Section: Mouse Workmentioning

confidence: 99%

“…Double heterozygotes resulting from these crosses were bred to Sox1 +/− mice. Appropriately staged embryos and their extra-embryonic tissues were collected, and genomic DNA from extra-embryonic tissue was screened by PCR (Nishiguchi et al, 1998). The Pax6 1-NeuSey/+ allele was identified by a PCR fragment polymorphism producing a HincII restriction site.…”

Section: Mouse Workmentioning

confidence: 99%

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Pax6 is misexpressed in Sox1 null lens fiber cells

Donner

Episkopou

et al. 2007

Gene Expression Patterns

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Sox1 null lens fiber cells fail to elongate and have disrupted expression of gamma crystallin. We have evaluated the expression of Sox1 and Pax6 proteins during critical stages of lens morphogenesis, with particular focus on fiber cell differentiation. While Pax6 and Sox1 are co-expressed during early stages of fiber cell differentiation, Sox1 up-regulation coincides temporally with the down-regulation of Pax6, and these proteins therefore display a striking inverse expression pattern in the lens fiber cell compartment. Furthermore, Pax6 is inappropriately expressed in the fiber cells of Sox1 null mice and the Pax6 target, α5 integrin, is simultaneously misexpressed. Finally, we demonstrate a genetic interaction between Sox1 and Pax6, as Sox1 heterozygosity partially rescues the diameter of Pax6 Sey lenses by increasing the number of cells in the fiber cell compartment.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Mouse Workmentioning

confidence: 99%

Section: Mouse Workmentioning

confidence: 99%

See 3 more Smart Citations

Pax6 is misexpressed in Sox1 null lens fiber cells

Donner

Episkopou

et al. 2007

Gene Expression Patterns

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A conserved blueprint for the eye?

Treisman

1999

Bioessays

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Although the eyes of all organisms have a common function, visual perception, their structures and developmental mechanisms are quite diverse. Recent research on eye development in Drosophila has identified a set of putative transcription factors required for the earliest step of eye development, specification of the field of cells that will give rise to the eye. These factors appear to act in a hierarchy, although cross‐regulation may amplify the eye fate decision or promote progression to the next step. Surprisingly, homologous proteins are also involved in vertebrate eye development, suggesting that this regulatory network was present in a primitive common ancestor and that it has been adapted to control visual organ formation in multiple species. The identification of genes acting upstream and downstream of these transcription factors will contribute to our understanding of the establishment of a developmental field, as well as of the divergence of regulatory pathways controlling the formation of eye structures. BioEssays 21:843–850, 1999. © 1999 John Wiley & Sons, Inc.

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SOX genes

Lefebvre

Crombrugghe

2002

Wiley Encyclopedia of Molecular Medicine

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Sox1 directly regulates the gamma -crystallin genes and is essential for lens development in mice

Cited by 267 publications

References 29 publications

Pax6 is misexpressed in Sox1 null lens fiber cells

Pax6 is misexpressed in Sox1 null lens fiber cells

A conserved blueprint for the eye?

SOX genes

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