Sox17-Mediated Maintenance of Fetal Intra-Aortic Hematopoietic Cell Clusters

Nobuhisa, Ikuo; Osawa, Mitsujiro; Uemura, Mami; Kishikawa, Y.; Anani, Maha; Harada, Kaho; Takagi, Hiromichi; Saito, Kosuke; Kanai‐Azuma, Masami; Kanai, Yoshikatsu; Iwama, Atsushi; Taga, Tetsuya

doi:10.1128/mcb.01485-13

Cited by 31 publications

(55 citation statements)

References 57 publications

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“…The low level of SOX17 in haematogenic cells is consistent with literature reporting the downregulation of SOX17 during the EHT (Clarke et al, 2013;Nobuhisa et al, 2014). Similarly, single-cell analysis of hPSCderived cells documented a downregulation of SOX17 at the EHT point (Guibentif et al, 2017).…”

Section: Agm-like He D9-18supporting

confidence: 78%

Human haematopoietic stem cell development: from the embryo to the dish

et al. 2017

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Haematopoietic stem cells (HSCs) emerge during embryogenesis and give rise to the adult haematopoietic system. Understanding how early haematopoietic development occurs is of fundamental importance for basic biology and medical sciences, but our knowledge is still limited compared with what we know of adult HSCs and their microenvironment. This is particularly true for human haematopoiesis, and is reflected in our current inability to recapitulate the development of HSCs from pluripotent stem cells in vitro. In this Review, we discuss what is known of human haematopoietic development: the anatomical sites at which it occurs, the different temporal waves of haematopoiesis, the emergence of the first HSCs and the signalling landscape of the haematopoietic niche. We also discuss the extent to which in vitro differentiation of human pluripotent stem cells recapitulates bona fide human developmental haematopoiesis, and outline some future directions in the field.

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Section: Agm-like He D9-18supporting

confidence: 78%

Human haematopoietic stem cell development: from the embryo to the dish

et al. 2017

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“…In two separate studies, gene expression profiles of fetal liver-derived versus adult bone marrow-derived HSCs revealed that the Sox17 transcription factor is specifically expressed in fetal and neonatal (up to 4 weeks) HSCs, but not in adult HSCs (He et al, 2011; Kim et al, 2007). Sox17 is expressed in the hemogenic endothelium, emerging HSCs and also in the intra-aortic cell clusters (Clarke et al, 2013; Nobuhisa et al, 2014). Germline deletion of Sox17 causes severe fetal hematopoietic defects and an absence of definitive HSCs.…”

Section: Exploring the Possible Origins Of Hsc Heterogeneitymentioning

confidence: 99%

“…These data suggest that Sox17 is required for the maintenance of neonatal but not adult HSCs, and that Sox17 is a key determinant of the earliest stages of HSC identity. Additional members of the SOX family, Sox7 and Sox18 , are also expressed in the dorsal aorta of the mouse embryo and show similar effects in the formation of cell clusters with hematopoietic activity (Nobuhisa et al, 2014). Both Sox17 and Sox18 are significantly upregulated in hemogenic endothelial cells compared with endothelial cells isolated from the AGM at the time of the endothelial to hemogenic transition (Solaimani Kartalaei et al, 2015).…”

Section: Exploring the Possible Origins Of Hsc Heterogeneitymentioning

confidence: 99%

The many faces of hematopoietic stem cell heterogeneity

Crisan

Dzierzak

2016

Development

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Not all hematopoietic stem cells (HSCs) are alike. They differ in their physical characteristics such as cell cycle status and cell surface marker phenotype, they respond to different extrinsic signals, and they have different lineage outputs following transplantation. The growing body of evidence that supports heterogeneity within HSCs, which constitute the most robust cell fraction at the foundation of the adult hematopoietic system, is currently of great interest and raises questions as to why HSC subtypes exist, how they are generated and whether HSC heterogeneity affects leukemogenesis or treatment options. This Review provides a developmental overview of HSC subtypes during embryonic, fetal and adult stages of hematopoiesis and discusses the possible origins and consequences of HSC heterogeneity.

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“…Gfi1b , Myb , Cebpα , Pu.1 , Nfe2 and Ikzf1 ). The expression of Sox17 was recently identified in HE cells from both the mouse and human (Clarke et al, 2013; Nakajima-Takagi et al, 2013; Nobuhisa et al, 2014). Remarkably Sox17 is expressed at the highest levels in PS34CD45− cKit− cells with a pattern reminiscent of Prom1 expression (Figure 4E).…”

Section: Resultsmentioning

confidence: 99%

Hematopoietic Reprogramming In Vitro Informs In Vivo Identification of Hemogenic Precursors to Definitive Hematopoietic Stem Cells

et al. 2016

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Summary Definitive hematopoiesis emerges via an endothelial-to-hematopoietic transition in the embryo and placenta; however, the precursor cells to hemogenic endothelium are not defined phenotypically. We previously demonstrated that the induction of hematopoietic progenitors from fibroblasts progresses through hemogenic precursors that are Prom1+Sca1+CD34+CD45− (PS34CD45−). Guided by these studies we analyzed mouse placentas and identified a population with this phenotype. These cells express endothelial markers, are heterogeneous for early hematopoietic markers, and localize to the vascular labyrinth. Remarkably, global gene expression profiles of PS34CD45− cells correlate with reprogrammed precursors and establish a hemogenic precursor cell molecular signature. PS34CD45− cells are also present in intraembryonic hemogenic sites. After stromal co-culture, PS34CD45− give rise to all blood lineages and engraft primary and secondary immunodeficient mice. In summary, we show that reprogramming reveals a phenotype for in vivo precursors to hemogenic endothelium, establishing that direct in vitro conversion informs developmental processes in vivo.

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Sox17-Mediated Maintenance of Fetal Intra-Aortic Hematopoietic Cell Clusters

Cited by 31 publications

References 57 publications

Human haematopoietic stem cell development: from the embryo to the dish

Human haematopoietic stem cell development: from the embryo to the dish

The many faces of hematopoietic stem cell heterogeneity

Hematopoietic Reprogramming In Vitro Informs In Vivo Identification of Hemogenic Precursors to Definitive Hematopoietic Stem Cells

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