Sox2 expression in the visual system of two teleost species

DeOliveira-Mello, Laura; Lara, Juan M.; Arévalo, R.; Velasco, Almudena; Mack, Andreas F.

doi:10.1016/j.brainres.2019.146350

Cited by 11 publications

(18 citation statements)

References 74 publications

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“…This is along the lines of previous studies (Gorsuch et al, 2017;März, Chapouton, et al, 2010;März, Schmidt, et al, 2010). At the same time, and consistent with our data, Sox2 has been shown previously to be expressed in mature radial glia in the retina (DeOliveira-Mello et al, 2019;Surzenko et al, 2013).…”

Section: Discussionsupporting

confidence: 94%

“…The islet1 antibody is known to bind to a subset of neurons in the zebrafish CNS (Johnson et al, 2016). Sox2 antibodies were generated against mouse Sox2 peptide and label stem cells and glia in the fish retina (DeOliveira‐Mello et al, 2019). Glial Fibrillary acidic protein (GFAP), glutamine synthetase (GS), and PCNA antibodies have all been characterized and used on teleost brain (Grupp et al, 2010).…”

Section: Methodsmentioning

confidence: 99%

“…This fish species grows to a standard length of 10–12 cm with a corresponding manifold increase in brain volume. A. burtoni has been used as a model fish for growth and the neural basis of sexual behavior (Davis & Fernald, 1990; DeOliveira‐Mello et al, 2019; Desjardins et al, 2010; Johns & Fernald, 1981; Maruska & Fernald, 2018; Nikonov & Maruska, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Organization of radial glia reveals growth pattern in the telencephalon of a percomorph fish Astatotilapia burtoni

et al. 2021

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In the brain of teleost fish, radial glial cells are the main astroglial cell type. To understand how radial glia structures are adapting to continuous growth of the brain, we studied the astroglial cells in the telencephalon of the cichlid fish Astatotilapia burtoni in small fry to large specimens. These animals grow to a standard length of 10–12 cm in this fish species, corresponding to a more than 100‐fold increase in brain volume. Focusing on the telencephalon where glial cells are arranged radially in the everted (dorsal) pallium, immunocytochemistry for glial markers revealed an aberrant pattern of radial glial fibers in the central division of the dorsal pallium (DC, i.e., DC4 and DC5). The main glial processes curved around these nuclei, especially in the posterior part of the telencephalon. This was verified in tissue‐cleared brains stained for glial markers. We further analyzed the growth of radial glia by immunocytochemically applied stem cell (proliferating cell nuclear antigen [PCNA], Sox2) and differentiation marker (doublecortin) and found that these markers were expressed at the ventricular surface consistent with a stacking growth pattern. In addition, we detected doublecortin and Sox2 positive cells in deeper nuclei of DC areas. Our data suggest that radial glial cells give rise to migrating cells providing new neurons and glia to deeper pallial regions. This results in expansion of the central pallial areas and displacement of existing radial glial. In summary, we show that radial glial cells can adapt to morphological growth processes in the adult fish brain and contribute to this growth.

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Section: Discussionsupporting

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Organization of radial glia reveals growth pattern in the telencephalon of a percomorph fish Astatotilapia burtoni

et al. 2021

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“…Sox2 is known to be expressed in progenitor cells that keep their stem cells properties in adults (DeOliveira-Mello et al, 2019). ScSox2 in situ labelling allowed us to clearly establish the boundary between the CMZ and TZ within the peripheral retina in the juvenile retina (Figs.…”

Section: Resultsmentioning

confidence: 99%

“…The anti-GFAP and the anti-GS antibodies have been previously used in the retina of the juvenile catshark as markers of early and late glial cells, respectively (Sanchez-Farías and Candal, 2016) and their specificity has been tested by western blot in brain protein extracts of adult catsharks (Docampo-Seara et al, 2019). PCNA is present in proliferating cells and although its expression is stronger during the S phase, it persists along the entire cell cycle…”

Section: Specificity Of Antibodiesmentioning

confidence: 99%

Loss of active neurogenesis in the adult shark retina

Hernández-Núñez

Robledo

Mayeur

et al. 2020

Preprint

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Neurogenesis is the process by which progenitor cells generate new neurons. As development progresses neurogenesis becomes restricted to concrete neurogenic niches, where it persists during postnatal life. The retina of teleost fishes is thought to proliferate and produce new cells throughout life. Whether this capacity may be an ancestral characteristic of jawed vertebrates, shared with chondrichthyans, which diverged from osteichthyans prior to the gnathostome radiation is completely unknown. Previous work from our group revealed that the juvenile retina of the catshark Scyliorhinus canicula shows active proliferation and neurogenesis. Here, we compared the morphology and proliferative status of the retina between catshark juveniles and adults. Histological analyses revealed an important reduction in the size of the peripheral retina (where progenitor cells are mainly located), an increase in the thickness of the plexiform layers and a decrease in the thickness of the inner nuclear layer in adults. Contrary to what has been reported in teleost fish, we did not observe active mitotic activity in the catshark retina after sexual maturation, suggesting that there is no significant proliferation and neurogenesis in adult specimens. Based on these results, we carried out RNA-Sequencing (RNA-Seq) analyses comparing the retinal transcriptome of juveniles and adults, which revealed a statistically significant decrease in the expression of many genes involved in cell proliferation and neurogenesis in adult catsharks. Our RNA-Seq data provides an excellent resource to identify new signaling pathways controlling neurogenesis in the vertebrate retina.

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Zebrafish embryonically exposed to valproic acid present impaired retinal development and sleep behavior

DeOliveira‐Mello,

Baronio,

Panula

2023

Autism Research

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Prenatal exposure to valproic acid (VPA), a drug widely used to treat epilepsy and bipolar disorder, is an environmental risk factor for autism spectrum disorder (ASD). VPA has been used to reproduce the core symptoms of ASD in animal model organisms, including zebrafish. Visual system functioning is essential in the interpretation of social conditions and plays an important role of several behavioral responses. We hypothesized that behavioral deficits displayed by ASD patients may involve impaired visual processing. We used zebrafish as model organism to investigate the visual system after embryonic exposure to VPA using histological, behavioral and gene expression analysis. We analyzed the pineal gland of zebrafish and sleep‐like behavior to study how VPA exposure alters photo‐sensibility of zebrafish. VPA‐exposed zebrafish showed a delay in the development of the retina and optic nerve, which normalized at five days post fertilization. At larval stage, VPA‐exposed zebrafish showed sleep disturbances associated with a reduced number of serotonin‐producing cells of the pineal gland. In addition, the number of hypocretin/orexin (hcrt) expressing neurons in the rostral hypothalamus at 6 and 14 days post fertilization was reduced. In conclusion, we demonstrated that although VPA exposure leads to a delay in visual system development, it does not affect larval visual function. The novel finding that VPA alters significantly cells involved in sleep regulation and the sleep‐like state itself may be relevant for understanding sleep disturbances in ASD patients.

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Sox2 expression in the visual system of two teleost species

Cited by 11 publications

References 74 publications

Organization of radial glia reveals growth pattern in the telencephalon of a percomorph fish Astatotilapia burtoni

Organization of radial glia reveals growth pattern in the telencephalon of a percomorph fish Astatotilapia burtoni

Loss of active neurogenesis in the adult shark retina

Zebrafish embryonically exposed to valproic acid present impaired retinal development and sleep behavior

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