Soy isoflavones protect against H2O2-induced injury in human umbilical vein endothelial cells

Abstract

The aim of this study was to investigate the effects of soy isoflavones on the injury of human umbilical vein endothelial cells induced by H2O2. EVC‑304 cells were preincubated with soy isoflavones for 12 h, and then exposed to 100 µM H2O2 for 1 h. Cell viability was evaluated by a 3‑(4,5‑di‑methylthiazol‑2‑yl) 2,5‑diphenyltetrazolium bromide assay. The apoptosis of EVC‑304 cells was detected by Hoechst 33258 and Annexin‑V/propidium iodide staining. The oxidative stress‑related biochemical parameters were detected and the expression of apoptosis‑related proteins was examined by western blot analysis. The results showed that incubation with soy isoflavones caused a significant increase in the viability of EVC‑304 cells and a decrease in cell apoptosis induced by H2O2. Soy isoflavones also markedly enhanced the activities of superoxide dismutase and glutathione peroxidase, and reduced the level of malondialdehyde. Western blot analysis results show that soy isoflavones can modulate the activation of nuclear factor‑κB and the mitochondria‑mediated apoptosis signaling pathway. The results of this study indicated the potential biological relevance of soy isoflavones in the therapy of cardiovascular diseases.

In ovo injection of soy isoflavones on hatching performance and intestinal development of newly hatched chicks

“…In our trial, in ovo injection of different doses of ISF increased GSH-PX activity in the serum of chicks (Hajati et al, 2014;Khaligh et al, 2018). Furthermore, in vitro studies in human and pig cells challenged by hydrogen peroxide showed that ISF increased the activities and mRNA expression of SOD and GSH (Jin et al, 2015;Khaligh et al, 2018;Ma et al, 2015). In the current study, no change was observed in Ca, P, albumen, TP and ALP serum concentrations, which contrasts with Sahin et al (2007), who determined that ISP supplementation increased the serum concentrations of Ca and P and the activity of ALP in quail.…”

“…In our trial, in ovo injection of different doses of ISF increased GSH‐PX activity in the serum of chicks (Hajati et al, 2014; Khaligh et al, 2018). Furthermore, in vitro studies in human and pig cells challenged by hydrogen peroxide showed that ISF increased the activities and mRNA expression of SOD and GSH (Jin et al, 2015; Khaligh et al, 2018; Ma et al, 2015). Furthermore, Fan et al (2018) reported that GSH‐PX showed a slight increase in the liver of 21‐day‐old broilers when breeder fed DAI‐low supplemented.…”

Effects of whey and soy protein supplementation on inflammatory cytokines in older adults: a systematic review and meta-analysis

“…These results may be attributed to the bioactive substances in soy isoflavones (i.e. phenolic compounds, daidzein, and genistein) that exert antioxidant effects (79,80) through glutathione peroxidase regulation and reduction of ROS and malondialdehyde infiltration (81) . Although several soy isoflavone doses were administered in this systematic review, subgroup analysis based on dose was not feasible due to the low number of studies.…”

Isoflavones: Anti-Inflammatory Benefit and Possible Caveats

“…Lately, soy isoflavone has been shown to decrease the concentrations of ROS and malondialdehyde, as well as increase the mRNA expressions and activities of superoxide dismutase and glutathione peroxidase in porcine mammary gland cells and human umbilical vein endothelial cells challenged by hydrogen peroxide [ 75 , 76 ]. These interesting findings, along with a previous report [ 45 ], indicate that isoflavones are modulators of enzymatic antioxidants (besides ROS scavengers), and that nuclear factor-erythroid 2 (Nrf2)—a key transcription factor that leads to gene transcription of various antioxidant and phase II detoxifying enzymes—is associated with this modulation [ 77 , 78 ].…”