Soy peptide nanoparticles by ultrasound-induced self-assembly of large peptide aggregates and their role on emulsion stability

Zhang, Yuanhong; Zhou, Feibai; Zhao, Ming; Lin, Lianzhu; Zheng-xiang, Ning; Sun, Baoguo

doi:10.1016/j.foodhyd.2017.07.021

Cited by 117 publications

(69 citation statements)

References 50 publications

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“…The PDI of the four CS/PEP‐NPs ranged from 0.225 to 0.290, indicating a good particle size distribution. Zeta‐potential of four different CS/PEP‐NPs ranged from 21.7 to 24.8 mv, and the 30‐kDa CS/PEP‐NPs showed the highest zeta‐potential ( p < 0.05).The particle size of free peptide was 1,795 nm, which might be attributed to the aggregation of the peptide (Zhang et al, ).…”

Section: Resultsmentioning

confidence: 99%

Chitosan/sodium tripolyphosphate nanoparticles as efficient vehicles for enhancing the cellular uptake of fish-derived peptide

Zhao

et al. 2018

J Food Biochem

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Methodology to enhance the intestinal absorption of peptides is an important challenge due to their easily degradation and poor permeability across the intestinal epithelium. In this study, the fish‐derived peptide (DGDDGEAGKIG)‐loaded chitosan (CS) nanoparticles (CS/PEP‐NPs) were prepared and investigated in Caco‐2 monolayer model. The results indicated zeta potential of CS/PEP‐NPs increased with the increase in molecular weight of CS (10–50 kDa). Transmission electron microscopy images revealed the CS/PEP‐NPs were uniform spherical‐shaped nanoparticles with a diameter of 50–200 nm (150 kDa). Compared to other CS/PEP‐NPs, 150‐kDa CS/PEP‐NPs performed an outstanding apparent permeability coefficient (Papp, 2.29 × 10−5 cm s−1) and cumulative amount of peptide (120 min, 2,987 ng) in Caco‐2 cells. CS/PEP‐NPs could reduce the tight junction integrity of Caco‐2 cells and enhance the intracellular fluorescence intensities of fluorescein isothiocyanate‐labeled peptide. These findings suggest that chitosan nanoparticles are promising carriers to promote intestinal absorption of fish‐derived peptide via paracellular pathway mediated by tight junctions. Practical applications Chitosans are promising carriers to promote intestinal absorption of fish‐derived peptide. The 150‐kDa CS/PEP‐NPs performed an outstanding apparent permeability coefficient (Papp, 2.29 × 10−5 cm s−1) and cumulative amount of peptide (120 min, 2,987 ng) in Caco‐2 cells. CS/PEP‐NPs could reduce the tight junction integrity of Caco‐2 cells and enhance the peptide uptake by paracellular pathway. Chitosan nanoparticles can be developed as vehicles for enhancing the cellular uptake of peptide in food industry.

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Section: Resultsmentioning

confidence: 99%

Chitosan/sodium tripolyphosphate nanoparticles as efficient vehicles for enhancing the cellular uptake of fish-derived peptide

Zhao

et al. 2018

J Food Biochem

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“…This work highlighted that the combined use of sonochemistry and peptides can allow easy fabrication of nanoparticles, with potential application in nuclei acids and drug delivery. Zhang et al 53 rst reported the synthesis of soy peptide nanoparticles (SPN) from large soy protein aggregates formed aer proteolysis and thermal inactivation of soy protein isolates. The high intensity sonication (20 kHz) of large soy protein aggregates produced SPN of 100 nm with homogenous size distribution (PDI 0.20).…”

Section: Ultrasound-driven Assembly Of Synthetic Peptides and Proteinsmentioning

confidence: 99%

Sound methods for the synthesis of nanoparticles from biological molecules

et al. 2021

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“…As shown in Figure 4, the particle size of soy PIH was about 5-6 nm with a microstructure presented as aggregated nanoparticles with a length of about 43 nm (Figure 4A), wheat PIH particles were about 7.7-15.8 nm with an aggregated nanoparticle microstructure of about 130 nm long (Figure 4B) and the size of pea PIH particles was about 5.4-7.4 nm with a microstructure of aggregated nanoparticles of about 53 nm in length (Figure 4C). Klompong et al [44] reported the use of enzymatic hydrolysis in the modification of protein structures, which resulted in small peptides as nanoparticles with different sizes. Therefore, the nanoparticle size and TEM analyses of PPIH were conducted.…”

Section: Microstructure Analysismentioning

confidence: 99%

Antioxidative Activity of Soy, Wheat and Pea Protein Isolates Characterized by Multi-Enzyme Hydrolysis

et al. 2021

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Hydrolysis of protein by proteases produces small molecular weights (MWs) peptides as nanomaterials that are easily absorbed. This study investigated the physicochemical properties and antioxidant activity of three plant protein isolates (PIs) including soy, wheat and pea after multi-enzyme hydrolysis. The MWs, particle size and microstructure of PI hydrolysate (PIH) were determined by SDS-PAGE and MALDI-TOF-MS mass spectrometry, dynamic light scattering and transmission electron microscopy, respectively. Cell viability was determined in vitro using a mouse skeletal muscle cell line (C2C12) and crystal violet staining. The MWs and particle sizes of the three plant PIs were reduced after hydrolysis by three proteases (bromelain, Neutrase and Flavourzyme). The MWs of soy, wheat and pea PIH were 103.5–383.0 Da, 103.5–1146.5 Da and 103.1–1937.7 Da, respectively, and particle size distributions of 1.9–2.0 nm, 3.2–5.6 nm and 1.3–3.2 nm, respectively. All three plant PIHs appeared as aggregated nanoparticles. Soy PIH (100 μg/mL) provided better protection against H2O2-induced oxidative damage to C2C12 than wheat or pea PIH. In summary, soy PIH had the best antioxidant activity, and particle size than wheat PIH and pea PIH. Therefore, soy PIH might be a dietary supplement for healthy diet and medical applications.

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Soy peptide nanoparticles by ultrasound-induced self-assembly of large peptide aggregates and their role on emulsion stability

Cited by 117 publications

References 50 publications

Chitosan/sodium tripolyphosphate nanoparticles as efficient vehicles for enhancing the cellular uptake of fish-derived peptide

Chitosan/sodium tripolyphosphate nanoparticles as efficient vehicles for enhancing the cellular uptake of fish-derived peptide

Sound methods for the synthesis of nanoparticles from biological molecules

Antioxidative Activity of Soy, Wheat and Pea Protein Isolates Characterized by Multi-Enzyme Hydrolysis

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