Soy proteins reduce progression of a focal lesion and lipoprotein oxidiability in rabbits fed a cholesterol-rich diet

Castiglioni, Silvia; Manzoni, Cristina; D’Uva, Antonio; Spiezie, Rossella; Monteggia, E; Chiesa, Giulia; Sirtori, Cesare R.; Lovati, Maria Rosa

doi:10.1016/j.atherosclerosis.2003.08.012

Cited by 18 publications

(12 citation statements)

References 36 publications

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“…This important study showed that white lupin exerts a protective activity against the progression of atherosclerosis. Similar changes were observed treating the same model with soybean protein (Castiglioni, Manzoni, D'Uva, Spiezie, Monteggia, Chiesa, et al, 2003).…”

Section: Animal Studiessupporting

confidence: 70%

The health benefits of sweet lupin seed flours and isolated proteins

Arnoldi

Boschin

Zanoni

2015

Journal of Functional Foods

121

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Abstract.The interest for lupin is continuously growing: one driving force are the numerous studies showing it provides useful health benefits. This review discusses available literature in the area of dyslipidaemia, diabetes, and hypertension prevention, providing hints on the mechanism of action.The addition of lupin protein to the diet of different models of hypercholesterolaemia, such as rat, rabbit, hamster and pig, induce decreases of total and non-HDL cholesterol. The clinical investigations on the cholesterol lowering activity provided controversial results. Those involving hypercholesterolaemic subjects and based on improved lupin foods gave statistically significant total and/or LDL-cholesterol reductions: both protein and fibre are relevant. The moderate hypotensive activity observed in some studies is probably linked to digestion-released ACE-inhibitory peptides.The hypoglycaemic activity, observed in post-prandial studies, is due to gamma-conglutin, a specific protein fraction. All this information suggests that lupin seeds may become a source of ingredients of innovative functional foods.Abbreviations: ACE, angiotensin converting enzyme; Akt, protein kinase B; DBP; diastolic blood pressure; GSK3, glycogen synthase kinase 3; HDL-C, high-density-lipoprotein cholesterol;HMGCoA, 3-hydroxy-3-methylglutaryl coenzyme A; LDL-C, low-density-lipoprotein cholesterol; NL-LUP, narrow-leaf lupin protein isolate; NPS, non-starch polysaccharides; PI3K, phosphatidylinositol 3'-kinase; SBP, systolic blood pressure; SCAP, SREBP-cleavage activating protein; SREBP, sterol regulatory element binding protein; TAG, triacylglycerides; TOC, tocopherol; W-LUP, white lupin protein isolate.

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Section: Animal Studiessupporting

confidence: 70%

The health benefits of sweet lupin seed flours and isolated proteins

Arnoldi

Boschin

Zanoni

2015

Journal of Functional Foods

121

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“…It is one of the most important crop plants for seed protein and oil content. Since a long time, consumption of soy food, and especially soy proteins, was associated with nutritional and health benefits for human, including hypocholesterolemic effect (Sugano et al, 1988), prevention of heart (Castiglioni et al, 2003) and breast (Barnes, Grubbs, Setchell, & Carlson, 1990) diseases. Many studies have also been conducted on soybean protein hydrolyzates produced with various enzymes such as microbial, gastric and pancreatic enzymes during the last four decades.…”

Section: Introductionmentioning

confidence: 98%

Screening of in vitro bioactivities of a soy protein hydrolysate separated by hollow fiber and spiral-wound ultrafiltration membranes

Roblet

Amiot

Lavigne

et al. 2012

Food Research International

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“…However, in vitro interaction between the 7S soybean globulin and thioredoxin, a small multifunctional protein with a redox-active disulfide-dithiol in the conserved active site sequence Cys-Gly-Pro-Cys, has been shown [3]. This finding might explain the longer lag phase of LDL oxidation induced by cupric oxide observed in rabbits fed cholesterol-rich diet containing soybean protein vs. that found in rabbits fed the same diet but containing casein as protein source [22].…”

Section: Discussionmentioning

confidence: 93%

Cloning, yeast expression, purification and biological activity of a truncated form of the soybean 7S globulin α′ subunit involved in Hep G2 cell cholesterol homeostasis

Consonni

Lovati

Manzoni

et al. 2010

The Journal of Nutritional Biochemistry

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A truncated form of alpha' chain (talpha'), the soybean 7S globulin subunit previously demonstrated to be active in controlling the cholesterol and triglyceride homeostasis in in vitro and in vivo models, was cloned and expressed in the yeast Pichia pastoris. The recombinant polypeptide spanned 216 amino acid residues from the N-terminal side and included the N-terminal extension region of the soybean subunit. The talpha' polypeptide was purified by conventional biochemical techniques, and its potential to modulate the activity of low-density lipoprotein (LDL) receptor was evaluated in a human hepatoma cell line (Hep G2) by monitoring the uptake and degradation of labeled LDL. The LDL uptake (+192%) and degradation (+143%) by cells tested at the highest talpha' dose (8 microM) were similar to those found in cells incubated with 1 microM simvastatin, a potent inhibitor of cholesterol biosynthesis. The cell response to talpha' was found to be dose dependent. The use of a recombinant polypeptide ruled out the involvement of any other soybean component. These findings open new prospects in the studies aimed at identifying soybean regulatory (poly)peptide(s) and the mechanism involved in this biological response, as a gateway to their utilization for the management of human health.

show abstract

Soy proteins reduce progression of a focal lesion and lipoprotein oxidiability in rabbits fed a cholesterol-rich diet

Cited by 18 publications

References 36 publications

The health benefits of sweet lupin seed flours and isolated proteins

The health benefits of sweet lupin seed flours and isolated proteins

Screening of in vitro bioactivities of a soy protein hydrolysate separated by hollow fiber and spiral-wound ultrafiltration membranes

Cloning, yeast expression, purification and biological activity of a truncated form of the soybean 7S globulin α′ subunit involved in Hep G2 cell cholesterol homeostasis

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