Soy Saponins Meditate the Progression of Colon Cancer in Rats by Inhibiting the Activity of<i>β</i>-Glucuronidase and the Number of Aberrant Crypt Foci but Not Cyclooxygenase-2 Activity

Guo, Yuwei; Chen, Yue‐Hwa; Chiu, Wan‐Chun; Liao, Hsiang I.; Lin, Shyh‐Hsiang

doi:10.1155/2013/645817

Cited by 8 publications

(6 citation statements)

References 35 publications

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“…Meanwhile, all rats injected with AOM developed ACF containing different numbers of crypts ( Table 3 ). In agreement with previously published findings, ACF formation in the distal colon was significantly higher than the proximal colon [ 20 , 42 ]. Compared with the cancer control group, the administration of 5-FU or EEAML at 250 mg/kg or 500 mg/kg significantly suppressed the formation of ACF (79.5%, 61.2% and 72.5%, respectively).…”

Section: Resultssupporting

confidence: 93%

The Chemopotential Effect of Annona muricata Leaves against Azoxymethane-Induced Colonic Aberrant Crypt Foci in Rats and the Apoptotic Effect of Acetogenin Annomuricin E in HT-29 Cells: A Bioassay-Guided Approach

et al. 2015

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Annona muricata has been used in folk medicine for the treatment of cancer and tumors. This study evaluated the chemopreventive properties of an ethyl acetate extract of A. muricata leaves (EEAML) on azoxymethane-induced colonic aberrant crypt foci (ACF) in rats. Moreover, the cytotoxic compound of EEAML (Annomuricin E) was isolated, and its apoptosis-inducing effect was investigated against HT-29 colon cancer cell line using a bioassay-guided approach. This experiment was performed on five groups of rats: negative control, cancer control, EEAML (250 mg/kg), EEAML (500 mg/kg) and positive control (5-fluorouracil). Methylene blue staining of colorectal specimens showed that application of EEAML at both doses significantly reduced the colonic ACF formation compared with the cancer control group. Immunohistochemistry analysis showed the down-regulation of PCNA and Bcl-2 proteins and the up-regulation of Bax protein after administration of EEAML compared with the cancer control group. In addition, an increase in the levels of enzymatic antioxidants and a decrease in the malondialdehyde level of the colon tissue homogenates were observed, suggesting the suppression of lipid peroxidation. Annomuricin E inhibited the growth of HT-29 cells with an IC50 value of 1.62 ± 0.24 μg/ml after 48 h. The cytotoxic effect of annomuricin E was further substantiated by G1 cell cycle arrest and early apoptosis induction in HT-29 cells. Annomuricin E triggered mitochondria-initiated events, including the dissipation of the mitochondrial membrane potential and the leakage of cytochrome c from the mitochondria. Prior to these events, annomuricin E activated caspase 3/7 and caspase 9. Upstream, annomuricin E induced a time-dependent upregulation of Bax and downregulation of Bcl-2 at the mRNA and protein levels. In conclusion, these findings substantiate the usage of A. muricata leaves in ethnomedicine against cancer and highlight annomuricin E as one of the contributing compounds in the anticancer activity of A. muricata leaves.

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Section: Resultssupporting

confidence: 93%

The Chemopotential Effect of Annona muricata Leaves against Azoxymethane-Induced Colonic Aberrant Crypt Foci in Rats and the Apoptotic Effect of Acetogenin Annomuricin E in HT-29 Cells: A Bioassay-Guided Approach

et al. 2015

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“…Modelos animales y celulares sugieren que las saponinas contenidas en las legumbres podrían estar mediando este efecto protector sobre el epitelio intestinal. En particular, una dieta suplementada con harina de garbanzo (10%) por 10 semanas fue efectiva para disminuir un 64% la formación de focos aberrantes de criptas colónicas en un modelo murino de tumores inducidos 65,66 . Experimentalmente, la incubación con apigenina (0-50 μM) por 24 horas en células HCT116 resultó en el arresto celular de esta línea celular, un efecto mediado por el aumento en la expresión de proteínas p53, p21CIP1 y WAF1, las cuales inhiben a la quinasa dependiente de ciclina 30,67 .…”

Section: Cáncer Colo-rectalunclassified

Una mirada actualizada de los beneficios fisiológicos derivados del consumo de legumbres

2018

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Las legumbres han sido históricamente consideradas una apetecible fuente de proteínas y fibra. En estos tiempos caracterizados por una tendencia epidemiológica al sobrepeso y a la obesidad, corregir hábitos poco saludables es prioritario. La incorporación de legumbres al menú cotidiano como sustituto de carnes podría significar, a mediano y largo plazo, aminorar la prevalencia de enfermedades crónicas no-transmisibles. Este trabajo ofrece una mirada actualizada de algunos constituyentes claves presentes en las semillas de legumbres frecuentemente consumidas por la población chilena: porotos (Phaseolus vulgaris L.), lentejas (Lens culinaris L.); garbanzos (Cicer arietinum L.) y arvejas (Pisum sativum L.). Con una perspectiva realista, se expone el efecto del remojo y la cocción, ambos procesos simples utilizados frecuentemente en su preparación. Además, se ha considerado la digestión y fermentación como procesos claves en la liberación de compuestos bioactivos y su interacción con la microbiota residente en la porción distal del tracto gastrointestinal. Finalmente, estudios epidemiológicos en conjunto con datos experimentales permiten obtener una idea de los mecanismos que subyacen al impacto nutricional que tiene el consumo habitual de legumbres.

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“…The result of this study appears to be in line with previous studies, which reported a higher number of ACF in distal parts of the colon compared to the proximal parts. 31 , 32 The consequent ACF detriment was significantly attenuated by administration of DECPR at 250 mg/kg and 500 mg/kg concentrations with an inhibitory percentage of 56.04% and 68.68%, respectively. A number of published findings on other Curcuma spp.…”

Section: Resultsmentioning

confidence: 96%

The chemopreventive potential of Curcuma purpurascens rhizome in reducing azoxymethane-induced aberrant crypt foci in rats

Rouhollahi¹,

Moghadamtousi²,

Al-Henhena³

et al. 2015

DDDT

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Curcuma purpurascens BI. rhizome, a member of the Zingiberaceae family, is a popular spice in Indonesia that is traditionally used in assorted remedies. Dichloromethane extract of C. purpurascens BI. rhizome (DECPR) has previously been shown to have an apoptosis-inducing effect on colon cancer cells. In the present study, we examined the potential of DECPR to prevent colon cancer development in rats treated with azoxymethane (AOM) (15 mg/kg) by determining the percentage inhibition in incidence of aberrant crypt foci (ACF). Starting from the day immediately after AOM treatment, three groups of rats were orally administered once a day for 2 months either 10% Tween 20 (5 mL/kg, cancer control), DECPR (250 mg/kg, low dose), or DECPR (500 mg/kg, high dose). Meanwhile, the control group was intraperitoneally injected with 5-fluorouracil (35 mg/kg) for 5 consecutive days. After euthanizing the rats, the number of ACF was enumerated in colon tissues. Bax, Bcl-2, and proliferating cell nuclear antigen (PCNA) protein expressions were examined using immunohistochemical and Western blot analyses. Antioxidant enzymatic activity was measured in colon tissue homogenates and associated with malondialdehyde level. The percentage inhibition of ACF was 56.04% and 68.68% in the low- and high-dose DECPR-treated groups, respectively. The ACF inhibition in the treatment control group was 74.17%. Results revealed that DECPR exposure at both doses significantly decreased AOM-induced ACF formation, which was accompanied by reduced expression of PCNA. Upregulation of Bax and downregulation of Bcl-2 suggested the involvement of apoptosis in the chemopreventive effect of DECPR. In addition, the oxidative stress resulting from AOM treatment was significantly attenuated after administration of DECPR, which was shown by the elevated antioxidant enzymatic activity and reduced malondialdehyde level. Taken together, the present data clearly indicate that DECPR significantly inhibits ACF formation in AOM-treated rats and may offer protection against colon cancer development.

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Soy Saponins Meditate the Progression of Colon Cancer in Rats by Inhibiting the Activity ofβ-Glucuronidase and the Number of Aberrant Crypt Foci but Not Cyclooxygenase-2 Activity

Cited by 8 publications

References 35 publications

The Chemopotential Effect of Annona muricata Leaves against Azoxymethane-Induced Colonic Aberrant Crypt Foci in Rats and the Apoptotic Effect of Acetogenin Annomuricin E in HT-29 Cells: A Bioassay-Guided Approach

The Chemopotential Effect of Annona muricata Leaves against Azoxymethane-Induced Colonic Aberrant Crypt Foci in Rats and the Apoptotic Effect of Acetogenin Annomuricin E in HT-29 Cells: A Bioassay-Guided Approach

Una mirada actualizada de los beneficios fisiológicos derivados del consumo de legumbres

The chemopreventive potential of Curcuma purpurascens rhizome in reducing azoxymethane-induced aberrant crypt foci in rats

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