Soya-cerebroside reduces interleukin production in human rheumatoid arthritis synovial fibroblasts by inhibiting the ERK, NF-κB and AP-1 signalling pathways

Lee, Hsiang-Ping; Wu, Yang‐Chang; Chen, Bo-Cheng; Liu, Shan‐Chi; Li, Te‐Mao; Huang, Wei-Chien; Hsu, Chin‐Jung; Tang, Chih‐Hsin

doi:10.1080/09540105.2020.1766426

Cited by 42 publications

(35 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mir-X™ miRNA First-Strand Synthesis and the SYBR ® RT-PCR kit were used for reverse transcription of miRNA. Analysis was carried out according to a previous protocol [ 36 – 38 ].…”

Section: Methodsmentioning

confidence: 99%

miR-let-7c-5p and miR-149-5p inhibit proinflammatory cytokine production in osteoarthritis and rheumatoid arthritis synovial fibroblasts

Law

Lee

Lin

et al. 2021

Aging

Self Cite

View full text Add to dashboard Cite

Osteoarthritis (OA) and rheumatoid arthritis (RA) are two of the most common types of arthritis. Both are characterized by the infiltration of a number of proinflammatory cytokines into the joint microenvironment. miRNAs play critical roles in the disease processes of arthritic disorders. However, little is known about the effects of miRNAs on critical inflammatory cytokine production with OA and RA progression. Here, we found higher levels of proinflammatory cytokines including interleukin 1 beta (IL-1β), interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) in human OA and RA synovial fibroblasts (SFs) compared with normal SFs. Searches of open-source microRNA (miRNA) software determined that miR-let-7c-5p and miR-149-5p interfere with IL-1β, IL-6 and TNF-α transcription; levels of all three proinflammatory cytokines were lower in human OA and RA patients compared with normal controls. Anti-inflammatory agents dexamethasone, celecoxib and indomethacin reduced proinflammatory cytokine production by promoting the expression of miR-let-7c-5p and miR-149-5p. Similarly, ibuprofen and methotrexate also enhanced miR-let-7c-5p and miR-149-5p expression in human SFs. The evidence suggests that increasing miR-let-7c-5p and miR-149-5p expression is a novel strategy for OA and RA.

show abstract

“…Mir-X™ miRNA First-Strand Synthesis and the SYBR ® RT-PCR kit were used for reverse transcription of miRNA. Analysis was carried out according to a previous protocol [ 36 – 38 ].…”

Section: Methodsmentioning

confidence: 99%

miR-let-7c-5p and miR-149-5p inhibit proinflammatory cytokine production in osteoarthritis and rheumatoid arthritis synovial fibroblasts

Law

Lee

Lin

et al. 2021

Aging

Self Cite

View full text Add to dashboard Cite

show abstract

“…Gene expression profile records were obtained from the GEO database (39632_at) for 50 normal prostate tissue samples and 52 prostate cancer tissue samples for analysis of MMP-13 expression (Hu et al, 2020;Lee et al, 2020).…”

Section: Analysis Of the Gene Expression Omnibus (Geo) Data Setmentioning

confidence: 99%

Melatonin impedes prostate cancer metastasis by suppressing MMP‐13 expression

Wang

Tai

Tang

et al. 2020

Journal Cellular Physiology

Self Cite

View full text Add to dashboard Cite

Prostate cancer has high metastatic potential. Men with higher urinary levels of the sleep hormone melatonin are much less likely to develop advanced prostate cancer compared with men with lower levels of melatonin. Melatonin has shown anticancer activity in experimental investigations. Nevertheless, the therapeutic effect of melatonin in metastatic prostate cancer has largely remained a mystery. Analyses of Gene Expression Omnibus data and human tissue samples indicated that levels of matrix metallopeptidase 13 (MMP‐13) expression are higher in prostate cancer patients than in healthy cancer‐free individuals. Mechanistic investigations revealed that melatonin inhibits MMP‐13 expression and the migratory and invasive capacities of prostate cancer cells via the MT1 receptor and the phospholipase C, p38, and c‐Jun signaling cascades. Importantly, tumor growth rate and metastasis to distant organs were suppressed by melatonin in an orthotopic prostate cancer model. This is the first demonstration showing that melatonin impedes metastasis of prostate cancer by suppressing MMP‐13 expression in both in vitro and in vivo models. Thus, melatonin is promising in the management of prostate cancer metastasis and deserves to undergo clinical investigations.

show abstract

“…In recent years, large amounts of natural products have been extracted by using different methods. These products consist of active compounds that have different efficacy, such as anticancer [ 31 , 32 ], anti-inflammation [ 33 , 34 ], and regulation of angiogenesis [ 10 , 35 , 36 ]. It shows that natural products are the potential source for drug discovery.…”

Section: Discussionmentioning

confidence: 99%

Enzyme-Digested Peptides Derived from Lates calcarifer Enhance Wound Healing after Surgical Incision in a Murine Model

et al. 2021

Self Cite

View full text Add to dashboard Cite

Surgical wounds are common injuries of skin and tissues and usually become a clinical problem. Until now, various synthetic and natural peptides have been widely explored as potential drug candidates for wound healing. Inhibition of the TNF-α signaling pathway and promotion of angiogenesis are suggested to be involved in their effects. Angiogenesis at the wound site is one of the essential requisites for rapid healing. In the present study, a novel peptide extract derived from the natural source Lates calcarifer, commonly known as sea bass or barramundi, was evaluated for its wound healing property. The specific acidic and enzymatic approaches were employed for producing sea bass extract containing small size peptides (molecular weight ranging from 1 kD to 5 kD). The cytotoxicity of the extract was examined in HaCaT and NIH3T3. After this, the effects of enzyme digested peptide extracts of sea bass on wound healing in mice were investigated. The peptide extracts (660 and 1320 mg/kg/day) and control protein (1320 mg/kg/day) was orally given to the wounded mice, respectively, for 12 days. The surgical method was improved by implanting a silicone ring at the wound site. The ring avoided the contracting effect in murine wounds, making it more closely related to a clinical condition. The results showed promising improvement at the wound site in mice. Sea bass peptide extracts accelerated the wound healing process and enhanced the microvessel formation at the wound site. The remarkable effects of this novel sea bass peptide extract in healing traumatic injuries revealed a new option for developing wound management.

show abstract

Soya-cerebroside reduces interleukin production in human rheumatoid arthritis synovial fibroblasts by inhibiting the ERK, NF-κB and AP-1 signalling pathways

Cited by 42 publications

References 37 publications

miR-let-7c-5p and miR-149-5p inhibit proinflammatory cytokine production in osteoarthritis and rheumatoid arthritis synovial fibroblasts

miR-let-7c-5p and miR-149-5p inhibit proinflammatory cytokine production in osteoarthritis and rheumatoid arthritis synovial fibroblasts

Melatonin impedes prostate cancer metastasis by suppressing MMP‐13 expression

Enzyme-Digested Peptides Derived from Lates calcarifer Enhance Wound Healing after Surgical Incision in a Murine Model

Contact Info

Product

Resources

About