Soybean genotype evaluation for iron deficiency chlorosis using sodium bicarbonate and tissue culture

Ünlü

In Vitro Cell.Dev.Biol.-Plant

et al. 2008

Nodal shoot segments of four grapevine genotypes well known for their Fe-chlorosis characteristic [Vitis vinifera × Vitis berlandieri Fercal, resistant; V. berlandieri × Vitis rupestris 1103 P, mid-resistant; Solonis (Vitis riparia × V. rupestris × Vitis candicans) × Othello (Vitis labrusca × V. riparia × V. vinifera) 1613 C, susceptible; V. vinifera L. cv. Perlette, resistant] were cultured in vitro. The effects of three levels of iron sodium ethylene-diaminotetraacetate (FeNaEDTA; 9, 18, 36 mg l −1 ) and three mixtures of iron and 840 mg l −1 NaHCO 3 (sodium bicarbonate) in the Murashige and Skoog (MS) medium supplemented 4.9 µM indole-3-butyric acid (IBA) were compared. We assayed the chlorosis rating of leaves, total chlorophyll of leaves, dry shoot weights of the plantlets, and active and total Fe content of the leaves. The most suitable concentration in determining the reaction of genotypes to iron chlorosis was 9 mg l −1 FeNaEDTA. Bicarbonate addition had negative effects on the iron intake and development of plants. While all genotypes were affected by non-ferrous conditions, Fercal and Perlette were found to be the most resistant genotypes and 1613 C rootstock as the most susceptible.The chlorosis rating of the tested genotypes ranked according to their known degree of tolerance and susceptibility to lime-induced chlorosis. The results of this study showed that the in vitro technique could successfully be used in viticulture to get results in shorter times in the studies, aiming at breeding new rootstocks and varieties suitable to calcareous soil conditions and determining the reactions of existing genotypes to Fe chlorosis.

Section: Introductionsupporting

confidence: 92%

Use of in vitro method to evaluate some grapevine varieties for tolerance and susceptibility to sodium bicarbonate-induced chlorosis

Ünlü

In Vitro Cell.Dev.Biol.-Plant

et al. 2008

Journal of Plant Nutrition

“…[3] Tissue culture techniques are also applied to screen plants which are tolerant to iron deficiency through exposure to low, intermediate and high Fe levels in the growth medium. [3,6,7] However, tissue culture has provided a time and labor saving method for screening chlorosis resistant genotypes. These advantages have made tissue culture the most appropriate approach in breeding programs for chlorosis resistant plants.…”

Section: Introductionmentioning

confidence: 99%

Growth and Micronutrient Acquisition of in Vitro Grown Bitter Almond and Sour Orange in Response to Iron Concentration From Different Iron Chelates

Shibli¹,

Mohammad²,

Ajlouni³

2002

Responses of microshoot growth and micronutrient acquisition in bitter almond (Amygdalus communis L.) and sour orange (Citrus aurantium L.) to different iron (Fe) concentrations (7.45, 14.90, 22.35, or 29.80 mg L À 1 ) added from FeEDTA or FeEDDHA were studied in vitro. Increasing FeEDTA concentration more than (22.35 mg L À 1 ) or using FeEDDHA as Fe source resulted in a significant decrease in most growth parameters (microshoot length, proliferation, and dry weight). Compared to the control, Fe and manganese (Mn) acquisition by microshoots increased with increasing Fe concentration in all treatments. On the other hand, copper (Cu) and zinc (Zn) acquisition showed a decrease

Journal of Plant Nutrition

“…Greenhouse methods involving soil (Fairbanks et al, 1987) or hydroponics (Byron and Lambert, 1983;Chaney et al, 1992) have been suggested. Laboratory methods involving tissue culture (Graham et al, 1992) or reductant release from roots (Stevens et al, 1993) have been proposed. Field screening trials are most common.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Soybean Cultivars for Resistance to Iron Deficiency Chlorosis in Rows Versus Hills

Goos

Johnson

2009

2 Selection of a resistant cultivar is the most practical control measure for iron deficiency chlorosis in soybean (Glycine max L. Merr.). Plant breeders routinely evaluate cultivars for chlorosis resistance in hill plots, but this procedure may overestimate the chlorosis resistance of a cultivar. The objective of this research was to compare the chlorosis scores of soybean cultivars differing in chlorosis resistance, planted in conventional 76-cm rows, or with two, four, or eight plants per hill. In both 2001 and2002, it was estimated that three plants per hill would give average chlorosis scores most similar to that observed in 76-cm rows. The highest overall precision was given with row plots, and the lowest with two plants per hill. Hill plots are more space-efficient than row plantings, but are much more easily lost due to animal predation.