Soybean glycinin decreased growth performance, impaired intestinal health, and amino acid absorption capacity of juvenile grass carp (Ctenopharyngodon idella)

Zhang, Yalin; Duan, Xuan‐Ming; Jiang, Wei‐Dan; Feng, Lin; Wu, Pei; Liu, Yang; Jiang, Jun; Kuang, Sheng‐Yao; Tang, Ling; Tang, Wu‐Neng; Zhou, Xiao‐Qiu

doi:10.1007/s10695-019-00648-z

Cited by 33 publications

(20 citation statements)

References 57 publications

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“…The AAT and PepT1 mRNA levels are closely correlated with the mediation of TOR signalling in fish (Wang et al., 2018; Zhang et al., 2019). TOR regulates the function of the translation initiation complex by altering the phosphorylation state of 4E‐BP1 and regulates the synthesis of ribosomal proteins and translational regulatory proteins by affecting the activity of S6K1 in animals (Feng et al., 2016; Fingar & Blenis, 2004; Inoki, Li, Zhu, Wu, & Guan, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Growth performance depends on the digestive and absorptive abilities of fish, which are determined by the digestive and brush border enzyme activities (Hakim, Uni, Hulata, & Harpaz, 2006; Wu et al., 2011). Fish growth performance is highly dependent on dietary proteins, which are hydrolysed by digestive enzymes into small peptides and amino acids (AA) and absorbed by small peptide, and/or free AA (FAA) transporters in fish intestines (Huang, Wen, Li, Xuan, & Zhu, 2017; Zhang et al., 2019). The positive effects of FAA and small peptide absorption catalysed by the brush border enzymes in the intestine on the growth performance of fish have been reviewed previously (Habte‐Tsion et al., 2015; Rønnestad & Conceição, 2012; Rungruangsak‐Torrissen et al., 2009; Wang et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, FAAs and small peptides are the predominant precursors of protein synthesis in organisms (Fern & Garlick, 1974; Hofland & de Jong, 2019) and their absorption relies mainly on the respective transporters in the intestine of animals (Bröer, 2008; Liu et al., 2013). The peptide transporter 1 (PepT1) in the animal gut is a primary transporter of small peptides (Chen, Wong, & Webb, 1999; Wang et al., 2018; Zhang et al., 2019). The amino acid transporters (AAT) can be divided into the following four primary types: neutral and cationic AAT (NCAAT) (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…SLC7A1) and anionic AAT (AAAT) (e.g. SLC1A3), which all play different roles in FAA transport in animals (Bröer, 2002, 2008; Wang et al., 2018; Zhang et al., 2019). Results from previous studies have shown that curcumin might regulate the four AATs and small peptide transporters.…”

Section: Introductionmentioning

confidence: 99%

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Dietary curcumin supplementation enhanced growth performance, intestinal digestion, and absorption and amino acid transportation abilities in on‐growing grass carp ( Ctenopharyngodon idella )

Zhou

Jiang

et al. 2020

Aquac Res

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This study investigated the effect of curcumin on growth performance, intestinal digestion, and absorption and amino acid transportation in on‐growing grass carp (Ctenopharyngodon idella). Curcumin at six levels (control (0), 120, 240, 360, 480 and 600 mg/kg) was included in the fish diets for 60 days. Dietary curcumin supplementation increased weight gain, final body weight, body length, percentage weight gain, specific growth rate, feed intake, feed efficiency, intestinal somatic index, intestinal protein content, intestine weight, folds height, digestion enzyme and brush border enzyme activities of the on‐growing grass carp. Notably, based on the quadratic regression of percentage weight gain, the most appropriate curcumin supplementation level was estimated to be 312.27 mg/kg in the diet of on‐growing grass carp. Curcumin diversely enhanced the mRNA abundance of neutral and cationic amino acid transporters, neutral amino acid transporters, cationic amino acid transporter, anionic amino acid transporters and peptide transporter 1 in the different segments of the fish intestine. In addition, curcumin up‐regulated the target of rapamycin (TOR) signalling pathway molecules. Therefore, curcumin was beneficial for the growth performance of fish by improving intestinal growth and development, intestinal digestion, and absorption, and amino acid transportation abilities.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dietary curcumin supplementation enhanced growth performance, intestinal digestion, and absorption and amino acid transportation abilities in on‐growing grass carp ( Ctenopharyngodon idella )

Zhou

Jiang

et al. 2020

Aquac Res

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“…The gene amplification efficiency was measured by the melting curve. The 2 −ΔΔCT method was used to calculate the gene expression as described by Yao et al ( 31 ) and Zhang et al ( 32 ).…”

Section: Methodsmentioning

confidence: 99%

Dietary Choline-Enhanced Skin Immune Response of Juvenile Grass Carp Might Be Related to the STAT3 and NF-kB Signaling Pathway (Ctenopharyngodon idella)

et al. 2021

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To investigate the effects and potential mechanisms of dietary choline on immune function in the skin of juvenile grass carp (Ctenopharyngodon idella), fish were fed different diets containing different levels of choline (142. 2, 407.4, 821.6, 1215.8, 1589.3, and 1996.6 mg/kg) for 70 d and then sampled after a 6-d challenge test. The results exhibited that dietary choline (1) advanced the contents of phosphatidylcholine (PC), betaine, and choline in grass carp skin (P < 0.05) and upregulated the mRNA abundance of choline transporter high-affinity choline transporter 1 (CHT1), choline transporter-like protein 1 (CTL1), and choline transporter-like protein 5 (CTL5), indicating that dietary choline could increase the contents of choline which might be connected with choline transporters in the grass carp skin; (2) receded skin rot symptom after infection with A. hydrophila (Aeromonas hydrophila), increased the levels of IgM, C4, and C3 and the activities of acid phosphatase (ACP) and lysozyme (LZ), raised mucin2, β-defensin, hepcidin, and LEAP-2B mRNA abundance (rather than LEAP-2A), downregulated pro-inflammatory cytokine mRNA abundance (IFN-γ2, IL-15, TNF-α, IL-6, IL-12P40, and IL-1β) in skin of juvenile grass carp (P < 0.05), and upregulated anti-inflammatory cytokine mRNA abundance (IL-10, IL-4/13A, TGF-β1, IL-11, and IL-4/13B) in grass carp skin (P < 0.05), demonstrating that choline enhanced the skin immune function; and (3) downregulated the mRNA abundance of IKKγ, NF-κBp52, IKKβ, c-Rel, NF-κBp65, STAT3b2, STAT3b1, JAK1, and JAK2 as well as protein level of NF-κBp65, p-STAT3 Tyr705, and p-STAT3 Ser727 in nucleus and inhibited the mRNA and protein level of IkBα (P < 0.05), indicating that choline-enhanced immune function might be relevant to the JAK1, 2 /STAT3, and NF-κB signaling pathway in fish skin. In conclusion, choline enhanced the skin immune function which might be related to JAK1, 2/STAT3, and NF-κB signaling molecules in fish. Furthermore, based on immune indices of grass carp (9.28–108.97 g) skin (C3 and IgM contents as well as ACP activities), the choline requirements were estimated to be 1475.81, 1364.24, and 1574.37 mg/kg diet, respectively.

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Soybean Glycinin Reduced Growth Performance and Antioxidant Capacity and Caused Intestinal Inflammation and Microbiome Changes in Large Yellow Croaker (Larimichthys crocea)

Shao,

Li,

You

et al. 2024

Food Frontiers

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Glycinin, a major anti‐nutrient in soybean meal, may trigger enteritis and oxidative stress in fish with overconsumption. However, its impact on intestinal inflammation and underlying signaling mechanisms in Larimichthys crocea remains unclear. The purpose of this study was to assess the effects of glycinin on growth, antioxidant function, inflammatory response, and gut microbiota. Four isonitrogenous and isolipidic experimental feeds were prepared, supplemented with 0%(G0), 3%(G3), 6%(G6), and 12%(G12) glycinin, respectively, and fed to juvenile L. crocea for 10 weeks. The findings indicated that dietary glycinin markedly decreased the growth, feed utilization, and survival rate of L. crocea. In addition, L. crocea fed with G6 and G12 diets showed lower trypsin activity compared with those fed with G0 and G3 diets. Similarly, fish fed with G6 and G12 diets showed higher malondialdehyde content and lower superoxide dismutase activity in the liver compared with those fed with G0 and G3 diets, suggesting that glycinin induced oxidative stress and led to the imbalance of the antioxidant system. Dietary glycinin significantly increased the mRNA expression levels of toll‐like receptors in the gut. Moreover, dietary glycinin resulted in a heightened expression of both C‐rel and p65 proteins, while also elevating the phosphorylation levels of JNK and ERK proteins, indicating that glycinin activated the MAPK/NF‐κB signaling route. In addition, dietary glycinin increased the abundance of pathogenic bacteria in the gut, including Bacteroides and Streptococcus, and reduced the Bifidobacterium abundance. The findings suggested that L. crocea is highly sensitive to glycinin, and a 3% level can cause growth decline and enteritis.

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Soybean glycinin decreased growth performance, impaired intestinal health, and amino acid absorption capacity of juvenile grass carp (Ctenopharyngodon idella)

Cited by 33 publications

References 57 publications

Dietary curcumin supplementation enhanced growth performance, intestinal digestion, and absorption and amino acid transportation abilities in on‐growing grass carp ( Ctenopharyngodon idella )

Dietary curcumin supplementation enhanced growth performance, intestinal digestion, and absorption and amino acid transportation abilities in on‐growing grass carp ( Ctenopharyngodon idella )

Dietary Choline-Enhanced Skin Immune Response of Juvenile Grass Carp Might Be Related to the STAT3 and NF-kB Signaling Pathway (Ctenopharyngodon idella)

Soybean Glycinin Reduced Growth Performance and Antioxidant Capacity and Caused Intestinal Inflammation and Microbiome Changes in Large Yellow Croaker (Larimichthys crocea)

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