Soybean GmNFYB1 transcription factor confers abiotic stress tolerance in transgenic Arabidopsis

Li, Wenbin; Mallano, Ali Inayat; Lei, Bo; Wang, Tao; Nisa, Zaibun; Li, Yongguang

doi:10.1139/cjps-2016-0158

Cited by 12 publications

(18 citation statements)

References 44 publications

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“…It is also worth mentioning that the drought-responsive GmNF-YA01 gene was highly expressed in nodules [ 29 ], suggesting that their orthologous chickpea partner CaNF-YA04 might have an important role associated with nodule functioning ( Figure 2 A,B). These candidate genes from chickpea and soybean could be selected for further in-depth studies for improvement of nitrogen-fixing efficiency that has been well-known to be adversely affected by water stress [ 55 , 56 , 57 ].…”

Section: Resultsmentioning

confidence: 99%

Identification, Structural Characterization and Gene Expression Analysis of Members of the Nuclear Factor-Y Family in Chickpea (Cicer arietinum L.) under Dehydration and Abscisic Acid Treatments

Chu

Nguyen

Watanabe

et al. 2018

IJMS

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In plants, the Nuclear Factor-Y (NF-Y) transcription factors (TFs), which include three distinct types of NF-YA, NF-YB, and NF-YC TFs, have been identified to play key roles in the regulation of various plant growth and developmental processes under both normal and environmental stress conditions. In this work, a total of 40 CaNF-Y-encoding genes, including eight CaNF-YAs, 21 CaNF-YBs, and 11 CaNF-YCs, were identified in chickpea, and their major gene and protein characteristics were subsequently obtained using various web-based tools. Of our interest, a phylogenetically-based analysis predicted 18 CaNF-Ys (eight CaNF-YAs, seven CaNF-YBs, and three CaNF-YCs) that potentially play roles in chickpea responses to dehydration according to their close relationship with the well-characterized GmNF-Ys in soybean. These results were in good agreement with the enrichment of drought-responsive cis-regulatory motifs and expression patterns obtained from in silico analyses using publically available transcriptome data. Most of the phylogenetically predicted drought-responsive CaNF-Y genes (15 of 18) were quantitatively validated to significantly respond to dehydration treatment in leaves and/or roots, further supporting the results of in silico analyses. Among these CaNF-Y genes, the transcript levels of CaNF-YA01 and CaNF-YC10 were the most highly accumulated in leaves (by approximately eight-fold) and roots (by approximately 18-fold), respectively, by dehydration. Furthermore, 12 of the 18 CaNF-Y genes were found to be responsive to the most well-known stress hormone, namely abscisic acid (ABA), in leaves and/or roots, suggesting that these genes may act in chickpea response to dehydration in ABA-dependent manner. Taken together, our study has provided a comprehensive and fundamental information for further functional analyses of selected CaNF-Y candidate genes, ultimately leading to the improvement of chickpea growth under water-limited conditions.

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Section: Resultsmentioning

confidence: 99%

Identification, Structural Characterization and Gene Expression Analysis of Members of the Nuclear Factor-Y Family in Chickpea (Cicer arietinum L.) under Dehydration and Abscisic Acid Treatments

Chu

Nguyen

Watanabe

et al. 2018

IJMS

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“…NF-Y TFs function in affecting plant growth and development (Kwong et al, 2003;Miyoshi et al, 2003;Xu et al, 2005;Wenkel et al, 2006;Cai et al, 2007;Mu et al, 2008;Wei et al, 2010;Ballif et al, 2011;Stephenson et al, 2011;Hackenberg et al, 2012;Hou et al, 2014;Myers et al, 2016) and are essential in abiotic stress responses. Overexpression of NFYA5 (Li et al, 2008), GmNFYA3 (Ni et al, 2013),GmNFYB1 (Li et al, 2017), and NF-YB2 and NF-YB3 (Sato et al, 2019) conferred drought tolerance to transgenic Arabidopsis plants. Transgenic rice plants overexpressing OsNFYA7 (Lee et al, 2015), OsHAP2E (Alam et al, 2015), and Cdt-NFYC1 (Chen et al, 2015) showed enhanced tolerance to osmotic stresses.…”

Section: Introductionmentioning

confidence: 96%

GmNFYA13 Improves Salt and Drought Tolerance in Transgenic Soybean Plants

Tang

et al. 2020

Front. Plant Sci.

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NF-YA transcription factors function in modulating tolerance to abiotic stresses that are serious threats to crop yields. In this study, GmNFYA13, an NF-YA gene in soybean, was strongly induced by salt, drought, ABA, and H 2 O 2 , and suppressed by tungstate, an ABA synthesis inhibitor. The GmNFYA13 transcripts were detected in different tissues in seedling and flowering stages, and the expression levels in roots were highest. GmNFYA13 is a nuclear localization protein with self-activating activity. Transgenic Arabidopsis plants overexpressing GmNFYA13 with higher transcript levels of stressrelated genes showed ABA hypersensitivity and enhanced tolerance to salt and drought stresses compared with WT plants. Moreover, overexpression of GmNFYA13 resulted in higher salt and drought tolerance in OE soybean plants, while suppressing it produced the opposite results. In addition, GmNFYA13 could bind to the promoters of GmSALT3, GmMYB84, GmNCED3, and GmRbohB to regulate their expression abundance in vivo. The data in this study suggested that GmNFYA13 enhanced salt and drought tolerance in soybean plants.

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“…3). Moreover, some physiological indices, especially the SWP, were not reported in previous reports [6,27,32,37].…”

Section: Discussionmentioning

confidence: 79%

“…8). The functions of GmNFYA3 and GmNFYB1 were previously studied only in transgenic Arabidopsis seedlings [32,37]. In OE soybean plants, ABA-dependent genes (GmbZIP1 and GmWRKY46) and ABA-independent marker genes (GmDREB1, GmDREB2 and GmDREB3), which functioned in up-regulating drought tolerance in plants [45,49,51,53,54], maintained higher transcription levels compared with EV lines, whereas opposite effects occurred in RNAi plants (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Overexpression of GmNFYA5 confers drought tolerance to transgenic Arabidopsis and soybean plants

et al. 2020

BMC Plant Biol

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Background: Crop productivity is challenged by abiotic stresses, among which drought stress is the most common. NF-Y genes, especially NF-YA genes, regulate tolerance to abiotic stress. Results: Soybean NF-Y gene GmNFYA5 was identified to have the highest transcript level among all 21 NF-YA genes in soybean (Glycine max L.) under drought stress. Drought-induced transcript of GmNFYA5 was suppressed by the ABA synthesis inhibitor naproxen (NAP). GmNFYA5 transcript was detected in various tissues at vegetative and reproductive growth stages with higher levels in roots and leaves than in other tissues, which was consist with the GmNFYA5 promoter: GUS fusion assay. Overexpression of GmNFYA5 in transgenic Arabidopsis plants caused enhanced drought tolerance in seedlings by decreasing stomatal aperture and water loss from leaves. Overexpression and suppression of GmNFYA5 in soybean resulted in increased and decreased drought tolerance, respectively, relative to plants with an empty vector (EV). Transcript levels of ABA-dependent genes (ABI2, ABI3, NCED3, LEA3, RD29A, P5CS1, GmWRKY46, GmNCED2 and GmbZIP1) and ABA-independent genes (DREB1A, DREB2A, DREB2B, GmDREB1, GmDREB2 and GmDREB3) in transgenic plants overexpressing GmNFYA5 were higher than those of wild-type plants under drought stress; suppression of GmNFYA5 transcript produced opposite results. GmNFYA5 probably regulated the transcript abundance of GmDREB2 and GmbZIP1 by binding to the promoters in vivo. Conclusions: Our results suggested that overexpression of GmNFYA5 improved drought tolerance in soybean via both ABA-dependent and ABA-independent pathways.

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Soybean GmNFYB1 transcription factor confers abiotic stress tolerance in transgenic Arabidopsis

Cited by 12 publications

References 44 publications

Identification, Structural Characterization and Gene Expression Analysis of Members of the Nuclear Factor-Y Family in Chickpea (Cicer arietinum L.) under Dehydration and Abscisic Acid Treatments

Identification, Structural Characterization and Gene Expression Analysis of Members of the Nuclear Factor-Y Family in Chickpea (Cicer arietinum L.) under Dehydration and Abscisic Acid Treatments

GmNFYA13 Improves Salt and Drought Tolerance in Transgenic Soybean Plants

Overexpression of GmNFYA5 confers drought tolerance to transgenic Arabidopsis and soybean plants

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