Soybean Oil-Quality Variants Identified by Large-Scale Mutagenesis

Hudson, Karen A.

doi:10.1155/2012/569817

Cited by 26 publications

(21 citation statements)

References 34 publications

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“…Line no. 3 from Hudson (2012) corresponds to line 18412, and line 14698 has not been previously described. Both lines were observed to have a significant reduction in palmitic acid levels, and not observed to have any morphological phenotypes or obvious alterations in seed quantity or quality otherwise (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…Low palmitic mutant lines were identified by screening 5000 N‐nitroso‐N‐methyl urea (NMU)‐mutagenized soybean lines using fatty acid profiling by gas chromatography as previously reported (Hudson, 2012). The comparative analysis of both mutant lines shown in Table 1 was based on inbred mutant individuals (M 4 generation) harvested singly and phenotyped in five‐seed bulks.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously described high‐palmitic acid containing mutants isolated from a mutagenized soybean population that contained mutations in the KASIIA gene (Head et al, 2012; Hudson, 2012). In this work we describe two mutants with low levels of palmitic acid that contain novel and independent mutations in the soybean FATB1A gene.…”

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confidence: 99%

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New Alleles of FATB1A to Reduce Palmitic Acid Levels in Soybean

2016

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In conventional elite soybean [Glycine max (L.) Merr.] varieties palmitic acid typically constitutes 10% of the total seed oil. Palmitic acid is a saturated fat linked to increased cholesterol levels, and reducing levels of saturated fats in soybean oil is a target for soybean improvement. To identify novel and useful variation that could help in reducing palmitic acid levels in soybean, we screened a chemically mutagenized population. Two lines with reduced levels of seed palmitic acid were identified, and each line had a 30% reduction in palmitic acid. Both lines carried distinct mutations in the FATB1A (Glyma.05G012300) gene that co‐segregated with the reduced palmitic acid phenotype. One line carried a single base change that disrupted the splicing of the FATB1A mRNA, and the second contained a missense mutation (aspartic acid substituted for glycine) in a conserved domain of the FATB1 enzyme. Molecular markers were designed that will enable breeders to follow these new FATB1A mutant alleles in plant populations to facilitate gene stacking for oil improvement.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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New Alleles of FATB1A to Reduce Palmitic Acid Levels in Soybean

2016

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“…Comparing to animals, there are fewer studies on the mutagenic effects of MNU on plants (Kurowska et al 2012). MNU has been used in breeding program of rice (Satoh et al 2010), barley (Jovtcher et al 2001), soybean (Hoffman et al 1999;Hudson 2012;Sebastian et al 1989) and wheat (Desai & Bhatia 1975). MNU is considered to be less harmful towards plant breeders because it is easily degraded once exposed to sunlight.…”

Section: Introductionmentioning

confidence: 99%

Effects of N-Methyl-N-Nitrosourea on Seed Germination of Stevia rebaudiana

Ahmad¹,

Zainuddin²,

Osman³

et al. 2019

JSM

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Stevia rebaudiana is a unique plant that contains non-caloric natural sweetener and has gained much interest among Malaysians. In this study, the effect of different concentrations of N-methyl-N-nitrosourea (MNU) was assessed in inducing mutation in Stevia seeds to produce genetic variations, which is valuable for crop improvement. Stevia seeds were soaked in six concentrations of MNU (0.0, 0.13, 0.25, 0.38, 0.50, and 1.00 mM) for four different durations (15, 30, 45, and 60 min) at room temperature. As a result, application of MNU reduced the germination percentage and germination rate of Stevia seeds as compared to the control group. Prolonged exposure to the highest concentration of MNU recorded the lowest percentage of germination (2.5 ± 1.4%) and the lowest germination rate (0.21 ± 0.16). Tricots were observed among seedlings treated with 0.13, 0.38 and 1.0 mM of MNU for 30 min. Presence of seedlings with albino colour proved the mutagenic effect of MNU on Stevia genome. Based on the percentage of seedlings with chlorophyll mutation, the most effective and efficient mutagenic treatment to induce mutation was 60 min in 0.25 mM of MNU.

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“…Several SACPD-C mutations and one SACPD-B mutant have been linked to elevated seed stearic acid phenotypes (Hammond and Fehr, 1983;Zhang et al, 2008;Boersma et al, 2012;Ruddle et al, 2012). Several elevated stearic acid germplasm lines have also been identified with unknown or unverified causal loci (Graef et al, 1985;Bubeck et al, 1989;Rahman et al, 1995;Hudson, 2012). Elevated stearic acid results in an increase in melting transition temperature in the triacylglycerols and phospholipids (Wang et al, 2001a).…”

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confidence: 99%

Agronomic Effects of Mutations in Two Soybean Δ9–Stearoyl‐Acyl Carrier Protein‐Desaturases

et al. 2013

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Soybean [Glycine max (L.) Merr.] oil normally contains 2 to 4% stearic acid. Oil with higher levels of stearic acid is desired for use in the baking industry for both its chemical properties and human health benefits. Several lines with increased stearic acid have been identified; however, the agronomic consequences of high seed stearic acid are poorly understood. This study evaluates the effects of high stearic acid mutant alleles for two different Δ9–stearoyl‐acyl carrier protein‐desaturase genes, SACPD‐B and SACPD‐C, on agronomic performance. Fifty F2–derived lines developed in a previous study were evaluated in a germination chamber and two field environments. Lines were either homozygous for the SACPD‐C fasnc allele previously identified in FAM94‐41, homozygous for the SACPD‐B fas2nc allele from the induced ‘Holladay’ mutant TCJWB03‐806‐7‐19, homozygous for both mutant genes, or homozygous wild‐type for both genes. SACPD‐C mutant lines were agronomically indistinguishable from the wild‐type lines and yielded as well (3534 vs. 3414 kg ha−1). SACPD‐B mutant lines suffered from a decreased seedling growth rate, were 15% shorter at maturity, had a 4% seed size reduction, and yielded 6% less than the wild‐type lines. The different agronomic performance between SACPD‐C and SACPD‐B mutant lines was attributed to altered fatty acid composition in vegetative tissues of the SACPD‐B mutant lines.

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Soybean Oil-Quality Variants Identified by Large-Scale Mutagenesis

Cited by 26 publications

References 34 publications

New Alleles of FATB1A to Reduce Palmitic Acid Levels in Soybean

New Alleles of FATB1A to Reduce Palmitic Acid Levels in Soybean

Effects of N-Methyl-N-Nitrosourea on Seed Germination of Stevia rebaudiana

Agronomic Effects of Mutations in Two Soybean Δ9–Stearoyl‐Acyl Carrier Protein‐Desaturases

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