Soybean QTL for Yield and Yield Components Associated with Glycine soja Alleles

Abstract

U.S. soybean [Glycine max (L.) Merr.] germplasm has a narrow genetic base. The objective of this study was to identify G. soja Sieb. and Zucc. alleles for yield and yield component quantitative trait loci (QTL). Two populations of BC2F4 lines were generated from a mating between recurrent parent G. max ‘7499’ and donor parent G. soja PI 245331 with one line in each population tracing back to the same BC2 plant. Population A was used for the QTL identification analysis and Population B was used for the QTL verification test. The Population A lines were genotyped at 120 simple sequence repeat marker loci and one phenotypic marker locus. There were 11 putative QTL significantly associated with yield and yield component traits across three environments. One QTL for seed yield was found using the combined data. At this locus, the G. soja allele at Satt511 on LG A1 was associated with increased seed yield with an additive yield effect of 191 to 235 kg ha−1 depending on the QTL analysis method. Across environments in the validation population, lines that were homozygous for the G. soja allele at Satt511 demonstrated a 6.3% yield increase over lines that were homozygous for the G. max allele. One seed filling period QTL was identified on LG F with an additive effect of +1 d. This QTL also provided a +1 d additive effect on maturity. These results demonstrate the potential of identifying positive alleles in the exotic germplasm of soybean.

Identification of positive yield QTL alleles from exotic soybean germplasm in two backcross populations

“…Wang et al (2004) reported four positive yield QTL alleles from G. soja PI 468916; however, the QTL were only identified when the significance threshold was reduced and the data were analyzed with simple linear regression. Li et al (2008) reported one positive yield QTL allele from G. soja and the QTL mapped to the same region on chromosome 5 where Kabelka et al (2004) also reported a yield QTL. Guzman et al (2007) identified eight positive yield QTL alleles from PIs but all of them mapped to the same regions where yield QTL were reported previously.…”

Conserved salt tolerance quantitative trait locus (QTL) in wild and cultivated soybeans

“…DNA marker analyses revealed that G. soja shows greater genetic diversity than G. max (Maughan et al 1995, Xu et al 2002, Xu and Gai 2003. Recently, several favorable traits, such as tolerance to dehydration (Chen et al 2006), high lutein content (Kanamaru et al 2006), seed protein electrophoretic variants (Fukuda et al 2005), and yield and yield components (Li et al 2008) have been identified in wild soybean, and demonstrated the usefulness of the wild soybean to improve cultivated soybean.…”

Identification and validation of quantitative trait loci for seed yield, oil and protein contents in two recombinant inbred line populations of soybean

“…They further assessed the QTL effects in various elite soybean genetic backgrounds, and found that the effect of the yield QTL was observed in only 2 of 6 genetic backgrounds. Li et al (2008) used two BC 2 F 4 populations to map and validate QTLs for yield and yield components in three environments. Eleven QTLs were mapped but only one QTL for seed yield, which was linked to the marker Satt511 on linkage group A1, was confirmed in the two populations.…”