Statistical analyses were conducted to investigate the nighttime medium‐scale traveling ionospheric disturbances (MSTIDs) for the first time by using airglow images and Global Positioning System (GPS) data over central China during 2013–2015. Our results show that the phase fronts of perturbations are aligned from northwest to southeast direction and propagate toward the southwest direction. The characteristics of the nighttime MSTIDs observed by OI 630.0 nm images are consistent with those of the nighttime MSTIDs obtained from the GPS data. The phase velocity, period, wavelength, and amplitude of nighttime MSTIDs are 50–150 m/s, 0.5–1.5 h, 150–400 km, and 2%–15%, respectively, as measured from 630.0 nm images and GPS data. In addition, we utilized the simultaneous observations from OI 630.0 nm and OI 557.7 nm images to explore the relationship between nighttime MSTIDs and gravity waves (<100 km) in the mesopause. It is found that the nighttime MSTIDs frequently occurred in the summer solstice, which was not consistent with the occurrence of gravity wave observed in the mesopause. Our results indicate that the nighttime MSTIDs may be generated by the coupling of electrodynamic processes rather than be trigged by gravity waves from the lower atmosphere.