Gaseous NH 3 removal was studied in laboratory-scale biofilters (14-L reactor volume) containing perlite inoculated with a nitrifying enrichment culture. These biofilters received 6 L/min of airflow with inlet NH 3 concentrations of 20 or 50 ppm, and removed more than 99.99% of the NH 3 for the period of operation (101, 102 days). Comparison between an active reactor and an autoclaved control indicated that NH 3 removal resulted from nitrification directly, as well as from enhanced absorption resulting from acidity produced by nitrification. Spatial distribution studies (20 ppm only) after 8 days of operation showed that nearly 95% of the NH 3 could be accounted for in the lower 25% of the biofilter matrix, proximate to the port of entry. Periodic analysis of the biofilter material (20 and 50 ppm) showed accumulation of the nitrification product NO 3 -early in the operation, but later both NO 2 -and NO 3 -accumulated. Additionally, the N-mass balance accountability dropped from near 100% early in the experiments to ~95 and 75% for the 20-and 50-ppm biofilters, respectively. A partial IMPLICATIONS Biofilters are becoming an acceptable and economical air pollutant control technology for treating air contaminated with low concentrations of certain gaseous volatile organic compounds (VOCs) and inorganic sulfur compounds. Although NH 3 removal in biofilters has also been demonstrated, the mechanism of removal has been unclear. This research demonstrates the mechanisms of NH 3 removal in biofilters and also some of the associated problems. Potential applications include improved treatment of waste gas streams containing NH 3 , such as those from wastewater treatment systems, animal houses, and composting facilities. Another specialized application considered here is the treatment of cabin air and exhaust gas streams from waste treatment, food processing, and plant growth operations for long-duration space missions, where it is important not only to treat the waste gases but also, if possible, to recover nutrients for recycling into biomass production.