Spaceflight Environment-Induced Variation in Root Yield and Active Constituents of Salvia miltiorrhiza

Abstract

Salvia miltiorrhiza is a significant source of bioactive compounds providing human health effects. Here, we surveyed root yield and the active constituents' divergences of second generation S. miltiorrhiza (SP2) responding to a spaceflight environment. High-performance liquid chromatography was conducted for the comprehensive constituents' characterizations of 28 SP2 lines (224 individuals) and the ground control (eight individuals). The results showed that the mean fresh and dry weight of roots ranged from 116 to 172 g and 25 to 119 g, respectively, in SP2 lines. In addition, the mean contents of four tanshinone compounds (tanshinone I, tanshinone IIA, cryptotanshinone, and dihydrotanshinone I) of 28 SP2 lines varied from 0.32 to 1.04 mg · g(-1), 0.47 to 2.39 mg · g(-1), 0.25 to 1.60 mg · g(-1), and 0.53 to 1.67 mg · g(-1), respectively. Except for salvianolic acid B, which varied drastically from 72 % to 201 % of the ground control treatment, the other six phenolic acid contents of the 28 SP2 lines all increased after spaceflight. Principal component analysis was performed to obtain an overview of the distribution of all samples, and score plots clearly separated the SP2 accessions from ground controls. Moreover, a positive relationship was observed between tanshinone I and tanshinone IIA (r = 0.790, p < 0.01), and rosmarinic acid was positively correlated with salvianolic acid B (r = 0.728, p < 0.01). In conclusion, this study demonstrated that a spaceflight environment induced SP2 accessions remarkably in the variation of root yield and active constituent content.

Quality Evaluation and Chemical Markers Screening of Salvia miltiorrhiza Bge. (Danshen) Based on HPLC Fingerprints and HPLC-MSn Coupled with Chemometrics

“…Danshen is used extensively as a raw material in the pharmaceutical and natural product industries. However, decreasing resources and insufficient active ingredients have become more and more serious, which have a negative impact on the internal quality and clinical application of Danshen [ 19 , 20 ]. In this case, a reliable and comprehensive methodology should be developed to validly evaluate their quality.…”

Integrative omic and transgenic analyses reveal the positive effect of ultraviolet‐B irradiation on salvianolic acid biosynthesis through upregulation of SmNAC1

“…SalAs were extracted with 70% methanol for 24 h in an ultrasonic water bath and analyzed by high‐performance liquid chromatography (HPLC) using the method previously described by Peng et al . (2014). Reference standards for salvianolic acid B (Chengdu Herbpurify CO, LTD, Sichuan, China) and rosmarinic acid (Chengdu Herbpurify CO, LTD) were used to determine the concentrations of SalAs.…”

Tanshinones, Critical Pharmacological Components in Salvia miltiorrhiza

“…Wild S. miltiorrhiza generally grows in central and northeastern China. Environmental and soil conditions affect the quality of Danshen and the content of active ingredients (Peng et al, 2014). The Chinese Flora reports that there are two varieties in the S. miltiorrhiza species, namely, the original variety ( S. miltiorrhiza var.…”