SPAD aptasensor for the detection of circulating protein biomarkers

Pasquardini, Laura; Pancheri, Lucio; Potrich, Cristina; Ferri, Alessandro; Piemonte, C.; Lunelli, Lorenzo; Napione, Lucia; Comunanza, Valentina; Alvaro, Maria; Vanzetti, Lia; Bussolino, Federico; Pederzolli, Cecilia

doi:10.1016/j.bios.2015.01.042

Cited by 27 publications

(8 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the long‐term stability of the proposed aptamer sensor was evaluted by measuring the response of 2.0 pM TB twice in a week for two months. For the regeneration of the sensor surface after each measurement, the aptamer sensor was dipped into a 0.2 M glycine‐hydrochloric acid (Gly‐HCl) buffer solution (pH=2.8) for 5 min 51. After three times washing with a HEPES solution, the aptamer sensor was incubated in the mixture of 2.0 pM TB and 16 μg/mL of TB/AuNPs/MB conjugate solutions.…”

Section: Resultsmentioning

confidence: 99%

Highly Sensitive Voltammetric Thrombin Aptamer Sensor Based on the Synergistic Effect of Doping/Depositing Gold Nanoparticles in Polydopamine Film

et al. 2015

View full text Add to dashboard Cite

[a] 1IntroductionAptamer basedb ioanalytical approaches with homogeneous and heterogeneous pathways represent promising tools for protein detection. Aptamers are synthetic DNA/ RNAb ased receptors synthesized from in vitro selection process [1][2][3].A ptamers with tridimensional conformation enables them to recognize biomolecules with affinities comparedt ot hoseo fa ntibodies [4][5][6][7].T hey can change conformation dependingo nt arget binding, which gives them great flexibility for designing novel biosensors with high sensitivitya nd selectivity.F abrication of biosensors with aptamers as biorecognitione lements were applied successfully and most of the reported works were related to thrombin (TB)-binding aptamer [8][9][10][11][12][13][14][15][16][17][18].T Bi s ap rotein that possesses many effectsi nc oagulation-related reactions responsible for blood clotting. It is as erine protease that converts soluble fibrinogen to insoluble strands of fibrin. It also plays av ital role in thrombosis and haemostasis and its detection is very usefulf or early diagnosis of intracerebral hemorrhage [19] and pulmonary metastasis [20].B ecause of clinical importance, ah ighly sensitive biosensor for the detection of TB would be of great importance.T Bd etection with aptamer based probe has severala dvantages in the area of biosensor [21].U ntil now,m any novel aptamer sensors based on fluorescence [22],s urface enhancedR aman spectroscopy [23],s urface plasmonr esonance [24],q uartz crystal microbalance [25] and electrochemistry [26,27] have been developed successfully.A mongt hem, electrochemical methods have been ap referable approachd ue to its high sensitivity,f ast response,l ow cost, and miniaturization. Although aptamer-based sensors (aptamer sensor) represent sensitive approaches,b ut the choice of am ore suitable aptamer immobilizing platformw ith sufficient biocompatibilitya nd conductivity is still ac hallenging task for the practicala pplication of aptamers ensors.Polydopamine (PD) is an ovel coating materials having extraordinary robust adhesion,w hich could be easilyd eposited all types of inorganic and organic surfaces with control film thickness and durable stability [28,29].A nother important advantage of this material is that it has ah igh biocompatibility [30].T hus,P Dc ould be an interesting material for the fabrication of ar obust electrochemical TB aptamers ensor. However, for an electrochemical aptamers ensor, the aptamer immobilizing platforms hould have sufficient conductivity in ordert of low the electron.S ince the conductivity of the PD film is low, thusi tn eeds to be increased for using as an aptameri mmobilizing platformi ne lectrochemical aptamer sensors. To increase the conductivity of the PD film, various nanomaterials can be usedfor the modificationo fthe film. Although, carbon nanotubes[ 10],q uantum dots [11],a nd gold nanoparticles (AuNPs) [12] were used for the fabrication of highly sensitiveT Ba ptamer sensors,h owever, the use of nanomaterials modified PD would be more advantag...

show abstract

Section: Resultsmentioning

confidence: 99%

Highly Sensitive Voltammetric Thrombin Aptamer Sensor Based on the Synergistic Effect of Doping/Depositing Gold Nanoparticles in Polydopamine Film

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Luminescence, as a general term is related to the energy transition between molecular orbitals that produces an emission of light. When the excitation of the molecules is caused by a chemical reaction, this light emission is chemiluminescence [ 27 , 33 , 36 , 58 , 71 , 81 ]. The emission that accompanies an electrochemical reaction is known as electrochemiluminescence [ 79 ].…”

Section: Detection Methods and Assay Formatsmentioning

confidence: 99%

Recent Microdevice-Based Aptamer Sensors

et al. 2018

View full text Add to dashboard Cite

Since the systematic evolution of ligands by exponential enrichment (SELEX) method was developed, aptamers have made significant contributions as bio-recognition sensors. Microdevice systems allow for low reagent consumption, high-throughput of samples, and disposability. Due to these advantages, there has been an increasing demand to develop microfluidic-based aptasensors for analytical technique applications. This review introduces the principal concepts of aptasensors and then presents some advanced applications of microdevice-based aptasensors on several platforms. Highly sensitive detection techniques, such as electrochemical and optical detection, have been integrated into lab-on-a-chip devices and researchers have moved towards the goal of establishing point-of-care diagnoses for target analyses.

show abstract

“…The Fab′ were immobilized on the Si 3 N 4 surface adapting the protocol described in Yoshimoto et al ., [ 11 ] with some modifications. In order to introduce thiol groups able to react with the cysteine groups on Fab′ fragments, the silicon nitride surface was functionalized in wet conditions with mercaptosilane (MPTMS) [ 12 ]. The surfaces (both aMZI chip and flat samples) were cleaned with an argon plasma (6.8 W, one min) to remove organic contaminants and to hydroxylate the surface and were then immersed in a 1% v/v solution of MPTMS in toluene anhydrous at 60 °C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Asymmetric Mach–Zehnder Interferometer Based Biosensors for Aflatoxin M1 Detection

et al. 2016

Self Cite

View full text Add to dashboard Cite

In this work, we present a study of Aflatoxin M1 detection by photonic biosensors based on Si3N4 Asymmetric Mach–Zehnder Interferometer (aMZI) functionalized with antibodies fragments (Fab′). We measured a best volumetric sensitivity of 104 rad/RIU, leading to a Limit of Detection below 5 × 10−7 RIU. On sensors functionalized with Fab′, we performed specific and non-specific sensing measurements at various toxin concentrations. Reproducibility of the measurements and re-usability of the sensor were also investigated.

show abstract

SPAD aptasensor for the detection of circulating protein biomarkers

Cited by 27 publications

References 39 publications

Highly Sensitive Voltammetric Thrombin Aptamer Sensor Based on the Synergistic Effect of Doping/Depositing Gold Nanoparticles in Polydopamine Film

Highly Sensitive Voltammetric Thrombin Aptamer Sensor Based on the Synergistic Effect of Doping/Depositing Gold Nanoparticles in Polydopamine Film

Recent Microdevice-Based Aptamer Sensors

Asymmetric Mach–Zehnder Interferometer Based Biosensors for Aflatoxin M1 Detection

Contact Info

Product

Resources

About