Spanish Broom (Spartium junceum L.) fibers impregnated with vancomycin-loaded chitosan nanoparticles as new antibacterial wound dressing: Preparation, characterization and antibacterial activity

Cerchiara, Teresa; Abruzzo, Angela; Palomino, Rogers Alberto Ñahui; Vitali, Beatrice; Rose, Renata De; Chidichimo, Giuseppe; Ceseracciu, Luca; Athanassiou, Athanassia; Saladini, Bruno; Dalena, Francesco; Bigucci, Federica; Luppi, Barbara

doi:10.1016/j.ejps.2016.11.028

Cited by 54 publications

(23 citation statements)

References 28 publications

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“…The DEE% of the VCM-StBAclm-Qt 1 quatsomes was 52.22 ± 8.4, which is higher compared to other previously reported formulated VCM vesicle systems with DLC% of 13.20 ± 1.17% [ 64 , 65 ]. This could be due to the ability of the VCM to partition, both in the lipidic bilayer and aqueous lumen of the quatsomes.…”

Section: Resultsmentioning

confidence: 55%

Formulation of pH-Responsive Quatsomes from Quaternary Bicephalic Surfactants and Cholesterol for Enhanced Delivery of Vancomycin against Methicillin Resistant Staphylococcus aureus

et al. 2020

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Globally, human beings continue to be at high risk of infectious diseases caused by methicillin-resistant Staphylococcus aureus (MRSA); and current treatments are being depleted due to antimicrobial resistance. Therefore, the synthesis and formulation of novel materials is essential for combating antimicrobial resistance. The study aimed to synthesize a quaternary bicephalic surfactant (StBAclm) and thereof to formulate pH-responsive vancomycin (VCM)-loaded quatsomes to enhance the activity of the antibiotic against MRSA. The surfactant structure was confirmed using 1H, 13C nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FT-IR), and high-resolution mass spectrometry (HRMS). The quatsomes were prepared using a sonication/dispersion method and were characterized using various in vitro, in vivo, and in silico techniques. The in vitro cell biocompatibility studies of the surfactant and pH-responsive vancomycin-loaded quatsomes (VCM-StBAclm-Qt1) revealed that they are biosafe. The prepared quatsomes had a mean hydrodynamic diameter (MHD), polydispersity index (PDI), and drug encapsulation efficiency (DEE) of 122.9 ± 3.78 nm, 0.169 ± 0.02 mV, and 52.22 ± 8.4%, respectively, with surface charge switching from negative to positive at pH 7.4 and pH 6.0, respectively. High-resolution transmission electron microscopy (HR-TEM) characterization of the quatsomes showed spherical vesicles with MHD similar to the one obtained from the zeta-sizer. The in vitro drug release of VCM from the quatsomes was faster at pH 6.0 compared to pH 7.4. The minimum inhibitory concentration (MIC) of the drug loaded quatsomes against MRSA was 32-fold and 8-fold lower at pH 6.0 and pH 7.4, respectively, compared to bare VCM, demonstrating the pH-responsiveness of the quatsomes and the enhanced activity of VCM at acidic pH. The drug-loaded quatsomes demonstrated higher electrical conductivity and a decrease in protein and deoxyribonucleic acid (DNA) concentrations as compared to the bare drug. This confirmed greater MRSA membrane damage, compared to treatment with bare VCM. The flow cytometry study showed that the drug-loaded quatsomes had a similar bactericidal killing effect on MRSA despite a lower (8-fold) VCM concentration when compared to the bare VCM. Fluorescence microscopy revealed the ability of the drug-loaded quatsomes to eradicate MRSA biofilms. The in vivo studies in a skin infection mice model showed that groups treated with VCM-loaded quatsomes had a 13-fold decrease in MRSA CFUs when compared to the bare VCM treated groups. This study confirmed the potential of pH-responsive VCM-StBAclm quatsomes as an effective delivery system for targeted delivery and for enhancing the activity of antibiotics.

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Section: Resultsmentioning

confidence: 55%

Formulation of pH-Responsive Quatsomes from Quaternary Bicephalic Surfactants and Cholesterol for Enhanced Delivery of Vancomycin against Methicillin Resistant Staphylococcus aureus

et al. 2020

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“…Lately, traditional wound dressings (such as bandages of cotton and wool) which passively offer wound safety, have been substituted by innovative dressings that are proficient in providing a favorable environment and distributing active ingredients to the wound to facilitate wound healing. In this respect, a plethora of materials and combinations, both synthetic or natural, have been utilized, with numerous formulations (e.g., sponges [ 10 ], hydrogels [ 62 ], films [ 63 , 64 , 65 ], hydrocolloids [ 66 ], hydrofiber mats [ 67 , 68 ], etc.) and properties that make them appropriate for the management of a specific wound.…”

Section: Ideal Properties Of Wound Dressingsmentioning

confidence: 99%

Treatment Strategies for Infected Wounds

2018

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The treatment of skin wounds is a key research domain owing to the important functional and aesthetic role of this tissue. When the skin is impaired, bacteria can soon infiltrate into underlying tissues which can lead to life-threatening infections. Consequently, effective treatments are necessary to deal with such pathological conditions. Recently, wound dressings loaded with antimicrobial agents have emerged as viable options to reduce wound bacterial colonization and infection, in order to improve the healing process. In this paper, we present an overview of the most prominent antibiotic-embedded wound dressings, as well as the limitations of their use. A promising, but still an underrated group of potential antibacterial agents that can be integrated into wound dressings are natural products, especially essential oils. Some of the most commonly used essential oils against multidrug-resistant microorganisms, such as tea tree, St. John’s Wort, lavender and oregano, together with their incorporation into wound dressings are presented. In addition, another natural product that exhibits encouraging antibacterial activity is honey. We highlight recent results of several studies carried out by researchers from different regions of the world on wound dressings impregnated with honey, with a special emphasis on Manuka honey. Finally, we highlight recent advances in using nanoparticles as platforms to increase the effect of pharmaceutical formulations aimed at wound healing. Silver, gold, and zinc nanoparticles alone or functionalized with diverse antimicrobial compounds have been integrated into wound dressings and demonstrated therapeutic effects on wounds.

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“…Also, the results of biochemical and microscopic studies indicated that TiNP ointment significantly reduced the number of total cells, macrophages, neutrophils and lymphocytes and significantly enhanced the number of blood vessels, fibrocytes, fibroblast/fibrocyte ratio and concentration of hydroxyproline, hexosamine and hexuronic acid compared to other study groups (Tables ). Studies have revealed that the amount of angiogenesis at the wound site increases with the incidence of the wound, which causes more migration of fibroblasts to this site . Then, the fibroblasts induce the production of collagen (proline, hydroxyproline and glycine) and extracellular matrix compounds like hexosamine and hexuronic acid .…”

Section: Resultsmentioning

confidence: 99%

Synthesis of titanium nanoparticles using Allium eriophyllum Boiss aqueous extract by green synthesis method and evaluation of their remedial properties

Seydi

Saneei

Jalalvand

et al. 2019

Applied Organom Chemis

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The application of nanotechnologies to medicine, or nanomedicine, which has already demonstrated its tremendous impact on the pharmaceutical and biotechnology industries, is rapidly becoming a major driving force behind ongoing developments in the antimicrobial and wound healing fields. This study confirms the potential of Allium eriophyllum Boiss aqueous extract for the green synthesis of titanium nanoparticles (TiNPs). Also, we reveal the antioxidant, cytotoxicity, cutaneous wound healing, antifungal and antibacterial properties of TiNPs. These nanoparticles were characterized using Fourier transform infrared (FT-IR) and UV-visible spectroscopies, X-ray diffraction (XRD), transmission electron microscopy (TEM) and field emission scanning electron microscopy (SEM). FT-IR findings suggested molecular interactions of compounds in the plant were the sources of reducing power, reducing titanium ions to TiNPs. In XRD analysis, 22.8 nm was measured for the crystal size of the nanoparticles. SEM and TEM images indicated a uniform spherical morphology and average diameters of 22 nm. 2,2-Diphenyl-1-picrylhydrazyl experiments were conducted to assess the antioxidant activities, which indicated similar antioxidant potentials for TiNPs and butylated hydroxytoluene. In the antimicrobial part of this study, agar diffusion experiments were done to determine the antibacterial and antifungal characteristics. TiNPs had antifungal activities against Candida guilliermondii, C. krusei, C. albicans and C. glabrata and antibacterial potentials against Gram-negative bacteria (Pseudomonas aeruginosa, Salmonella typhimurium and Escherichia coli O157:H7) and Gram-positive bacteria (Staphylococcus aureus, Streptococcus pneumoniae and Bacillus subtilis). Also, these nanoparticles did not have any cytotoxicity against human umbilical vein endothelial cells. In in vivo experiments, TiNP ointment significantly decreased (p ≤ 0.01) wound area, total cells, macrophages, neutrophils and lymphocytes and notably increased (p ≤ 0.01) wound contracture, vessels, hydroxyproline, hexosamine, hexuronic acid, fibrocytes and fibrocyte/fibroblast ratio in rats. The results of FT-IR, UV, XRD, TEM and SEM analyses confirm that aqueous extract of A. eriophyllum leaves can be used to yield TiNPs with notable antioxidant, antibacterial, antifungal and

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Spanish Broom (Spartium junceum L.) fibers impregnated with vancomycin-loaded chitosan nanoparticles as new antibacterial wound dressing: Preparation, characterization and antibacterial activity

Cited by 54 publications

References 28 publications

Formulation of pH-Responsive Quatsomes from Quaternary Bicephalic Surfactants and Cholesterol for Enhanced Delivery of Vancomycin against Methicillin Resistant Staphylococcus aureus

Formulation of pH-Responsive Quatsomes from Quaternary Bicephalic Surfactants and Cholesterol for Enhanced Delivery of Vancomycin against Methicillin Resistant Staphylococcus aureus

Treatment Strategies for Infected Wounds

Synthesis of titanium nanoparticles using Allium eriophyllum Boiss aqueous extract by green synthesis method and evaluation of their remedial properties

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