SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial Δψ in neuroblastoma cells

Tanpure, Smita; Boyineini, Jerusha; Gnanamony, Manu; Antony, Reuben; Fernández, Karen S.; Libes, Jaime; Lin, Julian; Pinson, David M.; Joseph, Pushpa A.; Gondi, Christopher S.

doi:10.3892/ol.2017.6075

Cited by 3 publications

(3 citation statements)

References 38 publications

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“…When the concentration of ROS and oxidative stress reaches a certain level, the Δψm would be changed, resulting in the release of apoptosis factors. 30 Here, the results showed that GCP-1 treatments induced the Δψm decline and indicated that the Δψm also played significant roles in the induction of apoptosis.…”

Section: Gcp-1 Induces Hela Cells Apoptosis Through Mitochondrial Pathwaysmentioning

confidence: 67%

Amino acid sequence identification of goji berry cyclic peptides and anticervical carcinoma activity detection

Guo

et al. 2021

Journal of Peptide Science

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The goji berry is widely used as tonics; however, the antihuman cervical carcinoma effect and underlying mechanism of goji berry peptide remain to be elucidated. The cyclic peptides are appealing targets in antitumor agent development, and in current study, three novel goji berry cyclic peptides (GCPs) were isolated and amino acid sequence identified. Among them, GCP-1 (Cycle-(Trp-Glu-His-Thr)) inhibited proliferation and induced human cervical cancer (HeLa) cells apoptosis and blocked the HeLa cells in G0/G1 phase significantly. Furthermore, the GCP-1 also inhibited the cervical carcinoma growth in vivo. Moreover, GCP-1 suppressed the cyclin expression and activated the caspase cascade and poly(ADP-ribose) polymerase. Of note, GCP-1 may be a promising novel inhibitor of human cervical cancer. Highlights• Three novel goji berry cyclic peptides (GCPs) were amino acid sequences identified, and antiproliferative activity was evaluated in three cervical cancer cell lines (C33A, SiHa, and HeLa) in vitro.• GCP-1 showed the most antiproliferative activity against HeLa cells with an IC 50 of 80.2 ± 7.93 μM (48 h).• GCP-1 induced the HeLa cell apoptosis and blocked the HeLa cells in G0/G1 phase.• GCP-1 suppressed the cyclin expression accompanied with the activation of the caspase cascade.

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Section: Gcp-1 Induces Hela Cells Apoptosis Through Mitochondrial Pathwaysmentioning

confidence: 67%

Amino acid sequence identification of goji berry cyclic peptides and anticervical carcinoma activity detection

Guo

et al. 2021

Journal of Peptide Science

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“…Several studies demonstrated that SPARC, GCSF, and IP‐10 were induced higher migration ability in trophoblast cells including various cell line. The SPARC regulates radiation induced HSP expression by inhibiting mitochondrial function, including mitochondrial mass and membrane potential in neuroblastoma cells, and by modulating the extracellular matrix (Furmento et al, 2016; Jiang et al, 2013; Tanpure et al, 2017; Zipin‐Roitman et al, 2007). The MCP‐1/‐3 is related to macrophage migration and infiltration through effects on mitochondrial lipid homeostasis.…”

Section: Discussionmentioning

confidence: 99%

Human placenta‐derived mesenchymal stem cells induce trophoblast invasion via dynamic effects on mitochondrial function

Seok

Jun

Cho

et al. 2021

Journal Cellular Physiology

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The trophoblast is a critical cell for placental development and embryo implantation in the placenta. We previously reported that placenta-derived mesenchymal stem cells (PD-MSCs) increase trophoblast invasion through several signaling pathways. However, the paracrine effects of PD-MSCs on mitochondrial function in trophoblasts are still unclear.Therefore, the objective of the study was to analyze the mitochondrial function of trophoblasts in response to cocultivation with PD-MSCs. The results showed that PD-MSCs regulate the balance between cell survival and death and protect damaged mitochondria in trophoblasts from oxidative stress. Moreover, PD-MSCs upregulate factors involved in mitochondrial autophagy in trophoblast cells. Finally, PD-MSCs improve trophoblast invasion. Taken together, the data indicate that PD-MSCs can regulate trophoblast invasion through dynamic effects on mitochondrial energy metabolism. These results support the fundamental role of mitochondrial energy mechanism in trophoblast invasion and suggest a new therapeutic strategy for infertility. K E Y W O R D S invasion, mitochondria, mitochondrial autophagy, placenta-derived mesenchymal stem cells, trophoblast 1 | INTRODUCTION Normal placentation requires adequate invasion of trophoblast cells into the maternal endometrium of the uterus. During pregnancy, trophoblasts play a role in embryonic and placental development through effects on mononuclear cytotrophoblast cell fusion and differentiation into syncytiotrophoblasts, which form the maternalfetal interface. Several studies have reported that numerous microenvironmental and cellular factors, including hypoxia inducible factor 1-alpha (HIF1-α), matrix metalloproteinase (MMP), and Rho family members, are involved in trophoblast invasion (Carter et al., 2015; Soares et al., 2017). First, transcription factors such as HIF1-α, which responds to hypoxia, are involved to placental development by stimulating trophoblast invasion. The overexpression of HIF1-α enhances trophoblast invasion via the autophagy pathway (Choi et al., 2012;Highet et al., 2015;Ietta et al., 2006). Second, MMPs are enzymes essential for invasion with the primary role of digesting the extracellular matrix. MMP-2 and MMP-9 are widely known as gelatinases that digest type IV collagen, the major collagen constituent of the basement membrane. MMP-2/9 deficiency leads to poorThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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“…Cell culture . Five human PCa cell lines DU145, LNCaP, PC-3, NPC-3 and BPC-3, as well as the control prostate epithelial cell line (RWPE-1 (19,20). The relative mRNA expression levels were normalized to the expression level of GAPDH using the 2 -∆∆Ct method.…”

Section: Methodsmentioning

confidence: 99%

Hypermethylation of the SPARC promoter and its prognostic value for prostate cancer

et al. 2017

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Secreted protein acidic and rich in cysteine (SPARC) is a secreted matricellular glycoprotein and plays a key role in the development of many tissues and organ types. However, the role of SPARC in prostate cancer (PCa) is still controversial. The aim of the present study was to investigate the abnormalities in the expression of SPARC and its promoter hypermethylation in prostate cancers and in correlated clinicopathological profiles. We examined the hypermethylation of the SPARC promoter as a potential mechanism for suppressing SPARC in PCa. The clinicopathological correlation between SPARC and its promoter expression and the prognostic significance of the aberrantly expressed genes were evaluated to identify novel biomarkers of PCa. SPARC expression was decreased in PCa cell lines, which correlated with hypermethylation of the SPARC promoter. Treatment with the demethylating agent 5-Aza-Cdr restored SPARC expression. Seventy percent (145 of 207) of the primary tumors exhibited SPARC hypermethylation, while only 2.6% was found in normal prostate mucosa (n=38). In PCa cases, SPARC hypermethylation was correlated with a poorer prognosis (P=0.005; relative risk 2.659, 95% CI, 1.433‑4.562). Our findings revealed potential diagnostic markers of PCa based on specific hypermethylated CpG sites and also provided new insights of SPARC as a novel biomarker and/or treatment modality for prostate cancer.

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SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial Δψ in neuroblastoma cells

Cited by 3 publications

References 38 publications

Amino acid sequence identification of goji berry cyclic peptides and anticervical carcinoma activity detection

Amino acid sequence identification of goji berry cyclic peptides and anticervical carcinoma activity detection

Human placenta‐derived mesenchymal stem cells induce trophoblast invasion via dynamic effects on mitochondrial function

Hypermethylation of the SPARC promoter and its prognostic value for prostate cancer

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