Sparing of radiation-induced damage to the physis: fractionation alone compared to amifostine pretreatment

Damron, Timothy A.; Spadaro, Joseph A.; Tamurian, Robert M.; Damron, Leatha A.

doi:10.1016/s0360-3016(00)00511-3

Cited by 30 publications

(34 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In Sprague-Dawley rats, HFRT resulted in further epiphyseal protection beyond conventional fractionation (Eifel 1988, Damron et al 2000. However, growth arrest was not completely eliminated with this approach, and the need for multiple sessions of anesthetic sedation limited its clinical use in young children.…”

Section: Introductionmentioning

confidence: 93%

“…Preclinical and clinical studies have demonstrated that epiphyseal growth arrest was reduced to some degree by use of either fractionated or hyperfractionated RT (HFRT) (Eifel 1988, Hartsell et al 1989, Eifel et al 1990, Alheit et al 1998, Damron et al 2000, Damron et al 2001. In Sprague-Dawley rats, HFRT resulted in further epiphyseal protection beyond conventional fractionation (Eifel 1988, Damron et al 2000.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Comparison of the protective effects of melatonin and amifostine on radiation-induced epiphyseal injury

Topkan¹,

Tufan²,

Yavuz³

et al. 2008

International Journal of Radiation Biology

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 98%

Comparison of the protective effects of melatonin and amifostine on radiation-induced epiphyseal injury

Topkan¹,

Tufan²,

Yavuz³

et al. 2008

International Journal of Radiation Biology

View full text Add to dashboard Cite

show abstract

“…The use of radioprotectant drugs has been suggested to minimize damage to the growth plate during irradiation for childhood extremity tumors Damron et al 2000;Pateder et al 2002;Damron et al 2003). Amifostine, a free radical scavenger, has been shown to be effective in growth plate radioprotection in vivo, providing significant reduction in growth loss from both single and fractionated doses of irradiation without affecting normal growth in an established growing rat model Damron et al 2000;Damron et al 2003).…”

mentioning

confidence: 99%

“…Amifostine, a free radical scavenger, has been shown to be effective in growth plate radioprotection in vivo, providing significant reduction in growth loss from both single and fractionated doses of irradiation without affecting normal growth in an established growing rat model Damron et al 2000;Damron et al 2003). Up to 57% of growth loss from a single irradiation dose has been recovered by amifostine at high doses in that model (Damron et al 2000). However, the effect of amifostine on growth plate growth factors and on antiapoptotic or pro-apoptotic molecules has not been examined.…”

mentioning

confidence: 99%

Temporal Changes in PTHrP, Bcl-2, Bax, Caspase, TGF-β, and FGF-2 Expression Following Growth Plate Irradiation With or Without Radioprotectant

Damron

Mathur

Horton

et al. 2004

J Histochem Cytochem.

Self Cite

View full text Add to dashboard Cite

S U M M A R YThis study examined temporal changes in growth plate apoptosis molecules and growth factors in an animal model of radiation injury with and without a radioprotectant. Thirty weanling 5-week Sprague-Dawley rats underwent right knee irradiation with single-fraction 17.5 Gy while the left served as internal control. Six animals each were sacrificed at 0.5, 1, 2, 3, or 4 weeks after irradiation. Half of the animals received pretreatment with amifostine (WR-2721) radioprotectant. Immunohistochemical staining for PTHrP, Bcl-2, Bax, caspase-3, FGF-2, and TGF-␤ was performed. PTHrP decreased to a nadir at 1 week after irradiation but rebounded to above control levels at 2 weeks in the reserve and transitional zones. The radioprotectant amifostine blunted the decrease in PTHrP but kept PTHrP expression lower than controls during the rebound phase in untreated irradiated animals. Hypertrophic zone Bax expression was decreased by amifostine in both irradiated and non-irradiated limbs at 1 and 2 weeks. FGF, TGF-␤ , Bcl-2, and caspase levels generally decreased at 1 week and returned thereafter toward control levels. These findings underscore the importance of PTHrP in response to growth plate irradiation and show the novel finding of a decrease in Bax expression with amifostine pretreatment.

show abstract

“…Accordingly, the topical administration of amifostine on the rectal mucosa may be a possible strategy to permit safe dose escalation of the drug and prevention of the side effects (such as hypotension, nausea, vomiting, allergic and skin reactions, etc. ), which can occur with systemic administration (11).…”

Section: Introductionmentioning

confidence: 99%

The Protective Effect of Amifostine on Radiation-Induced Proctitis: Systemic Versus Topical Application

Uzal¹,

Alas²,

Usta³

et al. 2012

Balkan Med J

View full text Add to dashboard Cite

Objective: The aim of the study was to evaluate the radioprotective efficacy of intrarectal administration of amifostine in radiation-induced proctitis compared to intraperitoneal administration. Materials and Methods:Thirty-two Sprague-Dawley rats were randomly divided into four groups: Control (CONT), irradiation alone (RT), intraperitoneal amifostine plus irradiation (IPAMI), and intrarectal amifostine plus irradiation (IRAMI). The rats in the RT, IPAMI and IRAMI groups were irradiated individually with a single dose of 17.5 Gy to the pelvis. Amifostine was administered by the intraperitoneal (200 mg/kg) or intrarectal (2000 mg/kg) route before irradiation. Histopathologic analysis of the rectum was performed 14 days after irradiation.Results: Significant radiation damage appeared in all histopathologic parameters and was reduced by amifostine. Pretreatment with IPAMI significantly reduced the inflammatory infiltrate in the lamina propria (p=0.021), cryptitis (p=0.002) and crypt abscess (p=0.015). However, the protective effect of IRAMI was significant for all parameters with equal or higher significance than IPAMI, including the eosinophil leucocytes count (p=0.02), and distortion of the crypts (p=0.008), and was also significant for regenerative/reparative atypia (p=0.013). Conclusion:Intrarectal high dose topical administration of amifostine is more effective in the prevention of radiation-induced proctitis compared to its intraperitoneal systemic administration.

show abstract

Sparing of radiation-induced damage to the physis: fractionation alone compared to amifostine pretreatment

Cited by 30 publications

References 22 publications

Comparison of the protective effects of melatonin and amifostine on radiation-induced epiphyseal injury

Comparison of the protective effects of melatonin and amifostine on radiation-induced epiphyseal injury

Temporal Changes in PTHrP, Bcl-2, Bax, Caspase, TGF-β, and FGF-2 Expression Following Growth Plate Irradiation With or Without Radioprotectant

The Protective Effect of Amifostine on Radiation-Induced Proctitis: Systemic Versus Topical Application

Contact Info

Product

Resources

About