Spasmolytic effect of Mentha pulegium L. involves ionic flux regulation in rat ileum strips

Estrada‐Soto, Samuel; González-Maldonado, Dalia; Castillo-España, Patricia; Aguirre‐Crespo, Francisco; Sánchez-Salgado, Juan Carlos

doi:10.1540/jsmr.46.107

Cited by 19 publications

(14 citation statements)

References 8 publications

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“…The A. repens extracts and Me fractions were effective in diminishing ileum peristalsis in a similar manner to the results reported elsewhere for Mentha pulegium L. (Lamiaceae) (Estrada-Soto et al, 2010). These results can help to provide a better understanding of the mechanism by which this plant ameliorates diarrhea.…”

Section: Discussionsupporting

confidence: 83%

Spasmolytic effect ofAlternanthera repenson isolated rat ileum

Garín-Aguilar

Benavides-Catalán

Cobos

et al. 2013

Pharmaceutical Biology

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Context: Alternanthera repens (L.) Kuntze (Amaranthaceae) is widely used in Mexican traditional medicine for the treatment of gastrointestinal disorders that are mainly related to diarrhea. Objective: The aim of the present study was to investigate the spasmolytic effect of hexane (Hx), methanol (Me) and aqueous (Aq) extracts as well as chromatographic Me fractions (F1-F6) of A. repens in rat ileum. Materials and methods: Dried and powdered aerial parts were used to obtain the extracts. The rat ileum preparations were incubated in Tyrode's solution gassed (95% O 2 -5% CO 2 ) at 37 C. The effect on the contractile response of isolated ileum was evaluated by obtaining cumulative concentration-response curves to CaCl 2 , KCl, 5-HT and acetylcholine in the absence and presence of different doses of Aq (0.56-2.09 mg/mL), Me (0.24-0.91 mg/mL) and Hx (0.24-0.91 mg/mL) extracts, as well as six Me fractions of 0.66 mg/mL (F1 to F6).

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Section: Discussionsupporting

confidence: 83%

Spasmolytic effect ofAlternanthera repenson isolated rat ileum

Garín-Aguilar

Benavides-Catalán

Cobos

et al. 2013

Pharmaceutical Biology

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“…The methanolic (Og) and hexanic (OgHE) extracts of Og were prepared according to a technique previously described by EstradaSoto and colleagues [16]. Og leaves were obtained at the Laboratório de Tecnologia Farmacêutica (LTF), Federal University of Paraíba, Brazil, and kept in a cool and airy environment for fifteen days for drying.…”

Section: O Gratissimum Linn and Its Polyphenolic Phytochemical Rosmmentioning

confidence: 99%

Ocimum gratissimum Linn. and rosmarinic acid, attenuate eosinophilic airway inflammation in an experimental model of respiratory allergy to Blomia tropicalis

Costa

Carneiro

Cerqueira-Lima

et al. 2012

International Immunopharmacology

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Allergic asthma has emerged as an important public health problem of urban populations in developed countries. Very often herbal medicine is used to treat this widespread disease, due to the lack of efficacy and the important side effects related to the classical drugs in use. Along this line, Ocimum gratissimum (Og) is a plant widely used in Brazilian folk medicine to treat inflammatory disorders, such as asthma. In the present study we evaluated the immunomodulatory effects of Og and rosmarinic acid (RA, a polyphenolic compound) in a murine model of respiratory allergy induced by the Blomia tropicalis (Bt) mite. The respiratory allergy was induced in A/J mice by administration of Bt extract and the treatment was done using 25, 50 or 100mg/kg of an Og methanolic extract or using 2, 20 or 200mg/kg of RA. We then evaluated the changes induced by these drugs on immunological parameters related to the allergic process, which are up-regulated in this allergic model. The treatment of animals with 100mg/Kg Og and 200mg/Kg RA led to a significant reduction in the numbers of leukocytes/eosinophils in bronchoalveolar lavage (BAL); eosinophil peroxidase activity in BAL; presence of mucus in respiratory tract, histopathological changes in the lung, and IL-4 in BAL. These results suggest that the methanolic extract of Og and the polyphenol RA have therapeutic potential in this murine model of respiratory allergy to a clinically relevant human sensitizer allergen.

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“…In the present study, the sustained contraction of the muscle caused by high concentration of The second messenger, nitric oxide, is an important candidate for mediating non-adrenergic and non-cholinergic smooth muscle relaxation in the gastrointestinal tract (Estrada et al, 1999;Takahashi, 2003;Estrada-Soto et al, 2010). Therefore, we explored the possibility that NO was involved in the antispasmodic activity displayed by citral and LE on the spontaneous contractions.…”

Section: Discussionmentioning

confidence: 94%

Spasmolytic effect of citral and extracts of Cymbopogon citratus on isolated rabbit ileum

Devi

Sim

Ismail

2011

J Smooth Muscle Res

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Cymbopogon citratus, commonly known as lemongrass, has been shown to have antioxidant, antimicrobial and chemo-protective properties. Citral, a monoterpenoid, is the major constituent of C. citratus that gives off a lemony scent and is postulated to be responsible for most of its actions. In addition, C. citratus has been traditionally used to treat gastrointestinal discomforts, however, the scientific evidence for this is still lacking. Thus, the aim of the present study was to investigate the effect of the extracts of various parts of C. citratus (leaves, stems and roots) and citral on the visceral smooth muscle activity of rabbit ileum. The effect of the test substances were tested on the spontaneous contraction, acetylcholine (ACh)-and KCl-induced contractions. Citral at doses between 0.061 mM to 15.6 mM and the extract of leaves at doses between 0.001 mg/mL to 1 mg/mL significantly reduced the spontaneous, ACh-and KCl-induced ileal contractions. When the ileum was incubated in K + -rich-Ca 2+ -free Tyrode's solution, it showed only minute contractions. However, the strength of contraction was increased with the addition of increasing concentrations of CaCl2. The presence of citral almost abolished the effect of adding CaCl2, while the leaf extract shifted the calcium concentration-response curve to the right, suggesting a calcium antagonistic effect. These results were similar to that elicited by verapamil, a known calcium channel blocker. In addition, the spasmolytic effect of citral was observed to be reduced by the nitric oxide synthase inhibitor, L-NAME. In conclusion, citral and the leaf extract of C. citratus exhibited spasmolytic activity and it appeared that they may act as calcium antagonists. Furthermore, the relaxant effect of citral, but not that of the leaf extract may be mediated by nitric oxide suggesting the presence of other chemical components in the leaf extract other than citral.

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Spasmolytic effect of Mentha pulegium L. involves ionic flux regulation in rat ileum strips

Cited by 19 publications

References 8 publications

Spasmolytic effect ofAlternanthera repenson isolated rat ileum

Spasmolytic effect ofAlternanthera repenson isolated rat ileum

Ocimum gratissimum Linn. and rosmarinic acid, attenuate eosinophilic airway inflammation in an experimental model of respiratory allergy to Blomia tropicalis

Spasmolytic effect of citral and extracts of Cymbopogon citratus on isolated rabbit ileum

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