The valorization of agricultural residues plays a fundamental role in renewable energy production. Particularly, the management of olive orchards in Lazio region generates a considerable amount of biomass that is currently unexploited, but it could represent a valid source of solid biofuel for energy production in the Lazio region (Italy). Using a Geographic Information System (GIS) approach entirely based on open source software, five suitable areas (A, B, C, D and E) have been selected as eligible for hosting and feeding a 1 MWe power plant. Harvesting and transportation costs were also calculated. The harvesting operation costs were EUR 96.79 Mgfm−1 in A, while they ranged from EUR 49.83 Mgfm−1 (E) up to EUR 56.51 Mgfm−1 (D) for the other sub-areas. Sub-area A showed also higher transport costs, EUR 21.55 Mgfm−1 while the same value ranged from EUR 14.75 Mgfm−1 (E) to EUR 16.59 Mgfm−1 (B) in the other sub-areas. However harvesting costs resulted higher than those reported in the literature, mainly due to the low pruning yield per surface unit, an aspect which is directly related to the olive grove’s management in the region where annual pruning is the usual practice. Future developments of the present study should encompass the social and environmental aspects of residual biomass supply chains herein proposed.