Spatial and temporal alterations of developing oligodendrocytes induced by repeated sevoflurane exposure in neonatal mice

Zhang, Zhihao; Liu, Huiqing; Jia, Sansan; Zhao, Jianshuai; Wang, Jiajia; Liu, Yang; Zhou, Fang; Li, Qi; Li, Zhaofei; Dong, Hailong; Zeng, Yi; Zhang, Rui; Zhong, Haixing

doi:10.1016/j.bbrc.2022.11.105

Cited by 6 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rotating test is a popular method to assess motor coordination function in mice [11]. The mice were taught on a spinning rod for ve minutes on the rst day at a steady speed of ve revolutions per minute (rpm).…”

Section: Rotarod Testmentioning

confidence: 99%

“…During spontaneous myelinogenesis, OPCs develop into mature oligodendrocytes (OLs) through activation, migratory recruitment, proliferation, and differentiation, leading to myelination [9,10]. In a recent study, the development of OPCs and the generation of myelin in the hippocampus mbria were impaired when a 7-day-old mouse was exposed to sevo urane [11].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rosiglitazone promotes oligodendrocyte development and myelin formation of repeated neonatal sevoflurane exposure via PPARγ signaling

Cao,

Jiang,

Wang

et al. 2024

Preprint

View full text Add to dashboard Cite

One of the most prevalent general anesthetics for kids and infants is sevoflurane. According to recent research, repeated exposure to sevoflurane in neonates induces cognitive and motor deficits. Peroxisome proliferator-activated receptor-γ (PPARγ) agonists have drawn plenty of attention recently as possible therapies for a variety of neurological conditions. In this research, we evaluated whether pretreatment with rosiglitazone in neonatal mice can repair myelination defects, cognitive impairment, and motor dysfunction via PPARγ. The mice were treated with 3% sevoflurane for two hours on postnatal days 6–8. The behavioral tests were conducted from P29 to P34. Additionally, we evaluated morphological and functional symptoms related to myelin.Our results showed that rosiglitazone pretreatment significantly ameliorated the cognitive and motor impairments of repeated neonatal sevoflurane exposure. Meanwhile, rosiglitazone pretreatment promoted oligodendrocyte precursor cell (OPCs) differentiation and myelination.This suggests that rosiglitazone may be used in clinical settings to increase the security of neonatal sevoflurane exposure. Furthermore, PPARγ and FASN may be mediators of rosiglitazone, which alleviates myelination defects, cognitive impairment, and motor dysfunction.

show abstract

Section: Rotarod Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rosiglitazone promotes oligodendrocyte development and myelin formation of repeated neonatal sevoflurane exposure via PPARγ signaling

Cao,

Jiang,

Wang

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Data revealed that clemastine enhanced myelination and could reverse cognitive dysfunction induced by isoflurane ( Li et al, 2019 ). Similarly, clemastine injected intraperitoneally (10 mg/kg/day) in mice on postnatal days 6 to 8 enhanced OLs maturation and myelination, and thus alleviated sevoflurane-induced cognitive impairment ( Zhang et al, 2022 ), indicating that although doses differed, long-term and short-term treatments with clemastine could rescue developmental cognitive impairment induced by anesthetics.…”

Section: Experimental Studies Of Clemastine In Environmental and Gene...mentioning

confidence: 99%

Insights on therapeutic potential of clemastine in neurological disorders

Jiang,

Wang,

Cao

et al. 2023

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Clemastine, a Food and Drug Administration (FDA)-approved compound, is recognized as a first-generation, widely available antihistamine that reduces histamine-induced symptoms. Evidence has confirmed that clemastine can transport across the blood–brain barrier and act on specific neurons and neuroglia to exert its protective effect. In this review, we summarize the beneficial effects of clemastine in various central nervous system (CNS) disorders, including neurodegenerative disease, neurodevelopmental deficits, brain injury, and psychiatric disorders. Additionally, we highlight key cellular links between clemastine and different CNS cells, in particular in oligodendrocyte progenitor cells (OPCs), oligodendrocytes (OLs), microglia, and neurons.

show abstract

“…Sevoflurane (Sev) is one of the most commonly used general anesthetics in pediatric anesthesia due to its advantages of rapid induction, low respiratory irritation, and rapid recovery ( Patel & Goa, 1996 ). Various animal studies have confirmed that repeated exposure to general anesthetics during development can result in long-term cognitive impairment, fine motor injury, and social impairment in mice ( Tang et al, 2021 ; Wu et al, 2020 ; Xu et al, 2021 ; Zhang et al, 2022b ; Zhang et al, 2023 ) and non-human primates ( Neudecker et al, 2021 ). However, many questions regarding the long-term effects of general anesthesia on brain development remain unanswered.…”

Section: Introductionmentioning

confidence: 99%

Integrated ribosome and proteome analyses reveal insights into sevoflurane-induced long-term social behavior and cognitive dysfunctions through ADNP inhibition in neonatal mice

Liang,

Liu,

Cao

et al. 2024

Zoological Research

View full text Add to dashboard Cite

A growing number of studies have demonstrated that repeated exposure to sevoflurane during development results in persistent social abnormalities and cognitive impairment. Davunetide, an active fragment of the activity-dependent neuroprotective protein (ADNP), has been implicated in social and cognitive protection. However, the potential of davunetide to attenuate social deficits following sevoflurane exposure and the underlying developmental mechanisms remain poorly understood. In this study, ribosome and proteome profiles were analyzed to investigate the molecular basis of sevoflurane-induced social deficits in neonatal mice. The neuropathological basis was also explored using Golgi staining, morphological analysis, western blotting, electrophysiological analysis, and behavioral analysis. Results indicated that ADNP was significantly down-regulated following developmental exposure to sevoflurane. In adulthood, anterior cingulate cortex (ACC) neurons exposed to sevoflurane exhibited a decrease in dendrite number, total dendrite length, and spine density. Furthermore, the expression levels of Homer, PSD95, synaptophysin, and vglut2 were significantly reduced in the sevoflurane group. Patch-clamp recordings indicated reductions in both the frequency and amplitude of miniature excitatory postsynaptic currents (mEPSCs). Notably, davunetide significantly ameliorated the synaptic defects, social behavior deficits, and cognitive impairments induced by sevoflurane. Mechanistic analysis revealed that loss of ADNP led to dysregulation of Ca 2+ activity via the Wnt/β-catenin signaling, resulting in decreased expression of synaptic proteins. Suppression of Wnt signaling was restored in the davunetide-treated group. Thus, ADNP was identified as a promising therapeutic target for the prevention and treatment of neurodevelopmental toxicity caused by general anesthetics. This study provides important insights into the mechanisms underlying social and cognitive disturbances caused by sevoflurane exposure in neonatal mice and elucidates the regulatory pathways involved.

show abstract

Spatial and temporal alterations of developing oligodendrocytes induced by repeated sevoflurane exposure in neonatal mice

Cited by 6 publications

References 22 publications

Rosiglitazone promotes oligodendrocyte development and myelin formation of repeated neonatal sevoflurane exposure via PPARγ signaling

Rosiglitazone promotes oligodendrocyte development and myelin formation of repeated neonatal sevoflurane exposure via PPARγ signaling

Insights on therapeutic potential of clemastine in neurological disorders

Integrated ribosome and proteome analyses reveal insights into sevoflurane-induced long-term social behavior and cognitive dysfunctions through ADNP inhibition in neonatal mice

Contact Info

Product

Resources

About